Agilent 7000 triple quad

GC-MS/MS (Agilent 7890A GC system,
Agilent 7000 Triple Quad, Agilent 7693 autosampler) in multiple reaction
monitoring mode (MRM) was used for identification and quantification
of 209 PCBs as 171 chromatographic peaks. The GC was equipped with
a Supelco SPB-Octyl capillary column (50% n-octyl,
50% methyl siloxane, 30 m × 0.25 mm ID, 0.25 μm film thickness)
with helium as carrier gas flowing at 0.75 mL/min and nitrogen as
collision gas. The GC operated in solvent vent injection mode at the
following injection conditions: initial temperature 45 °C, initial
time 0.06 min, ramp 600 °C/min to inlet temperature 325 °C
at 4.4 psi. The GC oven temperature program was 45 °C for 2 min,
45–75 °C at 100 °C/min and hold for 5 min, 75–150
°C at 15 °C/min and hold for 1 min, and 150–280 °C
at 2.5 °C/min and finally hold for 5 min (total run time 70.86
min). The triple quadrupole MS electron ionization source was set
to 260 °C. Additional details can be found in the accompanying
dataset deposited in the Iowa Research Online data repository.^{66 (link)}

Its microgreens (RM) grown in an open field and harvested at the fully expended green cotyledons stage which was 14 days from seed soaking, washing, and hulling (Abdallah 2008 (link)). Harvested RM was air-dried for 3 days according to previous study (Dzowela et al. 1995 ) and ground into powder. The phytochemical compounds present in RM powder were determined according to a previous method using gas chromatography-mass spectrometry GC/MS technique (Santana et al. 2013 (link)). The analysis was conducted using a GC (Agilent Technology 7890A) coupled with a mass selective detector (MSD, Agilent 7000 Triple Quad) equipped with Agilent HP-5 ms capillary column. The identification of components was based on a comparison of their mass spectra with the authentic compounds and by computer matching with the NIST library as well as by comparison of the fragmentation pattern of the mass spectral data with those registered in the literature. All local, national, or international guidelines and legislation were adhered to use of plants in this study.

PCB52 and its hydroxylated metabolites were extracted from tissues,
serum, and cecum and intestinal content using a published protocol;^{40 (link),41 (link)} see the Supporting Information. Briefly,
PCB52 and its metabolites were extracted from homogenates by liquid–liquid
extraction and cleaned by solid-phase extraction with acidified silica
gel. PCB52 and metabolites were quantified in the multiple reaction
monitoring setting (MRM) on an Agilent 7890 A GC system equipped with
an Agilent 7000 Triple Quad and Agilent 7693 autosampler (Agilent
Technologies, Santa Clara, CA); see the Supporting Information.