6 mercapto 1 hexanol

Manufactured by Merck Group

Sourced in United States, Germany, United Kingdom

6-mercapto-1-hexanol is a chemical compound used as a laboratory reagent. It has the chemical formula C6H14OS. The product is a clear, colorless liquid at room temperature.

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Lab products found in correlation

62 protocols using 6 mercapto 1 hexanol

Electrochemical Breast Cancer Detection

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The used primers and probes were designed based on the breast cancer cells obtained from the Gen Bank database. 6-Mercapto-1-hexanol (MCH) was purchased from Aldrich. HAuCl 4 and all other chemicals were of analytical grade and obtained from Merck Company. All of the chemicals were used as received without further purification. The sequences of the used probe and complementary were as follows:
Probe sequence (P1): 5'-AAGCGAGCAAGAGAATTCCAG-3' Complementary sequence (P1C): 5'-GTGAAAGTATCTAGCACTGCTGGAATTCT CTTGCTCGCTT-3' Non-complementary sequence (P1nC1): 5'-TGTGAAAGTATCTAGCACTGTGGGAAT-TCTCTTGCTCGCT-3' Non-complementary sequence (P1nC2): 5'-GAGAAACATCTGGGATA-3' Non-complementary sequence (P1nC3): 5'-CACTTTATTTGGGATG-3 For electrochemical measurements an Autolab potentiostat/galvanostat model PGSTAT 302 N (Eco Chemic, Utrecht, Netherlands) and NOVA 1.7 software at laboratory temperature (25 ± 1 °C) were used. The used three-electrode system was composed of a modified glassy carbon electrode as working electrode, an Ag/AgCl (1.0 mol L -1 KCl) and a platinum wire as reference and auxiliary electrodes, respectively. A Metrohm model 691 pH/mV meter was applied for pH measurements. The graphene nanosheets were synthesized according to the procedure given in the literature. 25 (link)

Benvidi A., Abbasi Z., Dehghan Tezerjani M., Banaei M., Zare H.R., Molahosseini H, & Jahanbani S. (2018). A Highly Selective DNA Sensor Based on Graphene Oxide-Silk Fibroin Composite and AuNPs as a Probe Oligonucleotide Immobilization Platform3667. Acta chimica Slovenica, 65(2).

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Lead Ion Aptamer Sensing Protocol

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potassium hexacyanoferrate (III) (K₃Fe(CN)₆), potassium hexacyanoferrate (II) (K₄Fe(CN)₆), methylene blue (MB), Tris-HCl, manganese(II) nitrate hydrate, uranyl acetate, lead nitrate, cadmium nitrate, mercury chloride, sodium selenate, nickel sulfate, potassium antimony(III) tartrate hydrate, arsenic(III) ICP standard, 6-mercapto-1-hexanol (MCH), and potassium dihydrogen phosphate were purchased from Aldrich Chemicals, Darmstadt, Germany. Nitrilotriacetic acid (NTA) was purchased from Alfa Aesar, Haverhill, MA, USA.
DNA aptamer probe (desalted, purified by HPLC) specific for lead ions and reference ssDNA sequences were purchased from Metabion, Planegg, Germany. The aptamer sequence was 5′-OH-C₆-S-S-C₆-GGT TGG TGT GGT TGG-3′ and the reference one was 3′-OH-C₃-S-S-C₃-TTT TTT TTT TTT TTT-5′. Aptamer stock solutions (100 μM) were prepared with nuclease-free water and stored in a −20 °C freezer before use.

Jarczewska M., Sokal M., Olszewski M, & Malinowska E. (2024). Studies on the Aptasensor Miniaturization for Electrochemical Detection of Lead Ions. Biosensors, 14(2), 110.

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DNA Hybridization Optimization Protocol

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Phosphate buffer (Sigma-Aldrich), sodium chloride (Sigma-Aldrich), 6-mercapto-1-hexanol (Sigma-Aldrich), and tris(2-carboxyethyl)phosphine hydrochloride (Molecular Probes, Carlsbad, CA) were all used as received. The probe and complement DNA sequences were synthesized commercially and used as employed (Biosearch Technologies, Novato, CA). The sequences we employed are as follows:

Kang D., Yu J., Xia F., Huang J., Zeng H., Tirrell M., Israelachvili J, & Plaxco K.W. (2021). Nanometer-Scale Force Profiles of Short Single- and Double-Stranded DNA Molecules on a Gold Surface Measured Using a Surface Forces Apparatus. Langmuir : the ACS journal of surfaces and colloids, 37(45), 13346-13352.

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Biomolecular Detection Protocol

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Materials and reagents. Magnesium chloride hexahydrate, sodium chloride, sodium carbonate anhydrous, sodium thiosulfate pentahydrate, sulphuric acid (95-98%), hydrochloric acid (37%), ethanol (≧99.7%), and nitric acid (>90%) were purchased from Beijing Chemical Reagent Co.
(Beijing, China). Tris (hydroxymethyl)-aminomethane, Tris (hydroxymethyl-aminomethane hydrochloride (Tris-HCl) and 6-mercapto-1-hexanol (MCH) were ordered from Sigma-Aldrich (Saint Louis, USA). Hexaammineruthenium (III) chloride (98%), Tris-(2-carboxyethyl) phosphinehydrochloride (TCEP), formaldehyde, and silver nitrate were purchased from Acros (Brussels, Belgium). Exo I and Alkaline Phosphatase were purchased from the Thermo Scientific (Lithuania).

Gao X., Geng M., Li Y., Wang X, & Yu H.Z. (2017). Revealing and Resolving the Restrained Enzymatic Cleavage of DNA Self-Assembled Monolayers on Gold: Electrochemical Quantitation and ESI-MS Confirmation. Analytical chemistry, 89(4).

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Electrochemical Immunosensor for PSA Detection

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Mouse monoclonal IgG antibody against PSA (Ab10187) was purchased from Abcam (UK), free-PSA (PSA) purified from human seminal fluid was obtained from Fitzgerald Industries International (USA). 11-mercaptoundecanoic acid (MUA), 6-mercapto-1-hexanol (MCH), ethanolamine, hydrogen peroxide (30% w/w), phosphate buffered saline (PBS) tablets, potassium chloride, potassium hexacyanoferrate(III), potassium hexacyanoferrate(II) trihydrate, sodium hydroxide, sulphuric acid (95.0 – 98.0%), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Sigma Aldrich (USA). Sambucus nigra agglutinin type I (SNA-I recognising α-2,6 linked sialic acid) lectin from elderberry was purchased from EY Laboratories (USA) and Maackia amurensis agglutinin (MAA recognizing α-2,3 linked sialic acid) lectin was obtained from Vector Laboratories (USA). Ethanol for UV/VIS spectroscopy (ultrapure) was purchased from Slavus (Slovakia). PBS solution (10 mM, pH 7.4) was prepared by dissolving 1 tablet in 200 mL of ultra-pure deionized water (DW). All solutions were filtered prior to use (0.2 μm sterile filters) and prepared in ultra-pure DW. Working solution of PSA and anti-PSA were prepared by dilution in PBS (10 mM, pH 7.4).

Pihíková D., Belický Š., Kasák P., Bertok T, & Tkac J. (2015). Sensitive detection and glycoprofiling of a prostate specific antigen (PSA) using impedimetric assays. The Analyst, 141(3), 1044-1051.

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Preparation of Hybridization Buffer

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6-Mercapto-1-hexanol
(MCH) was purchased from
Sigma-Aldrich (St. Louis, MO). All other chemicals were of analytical
grade and used without any further purification. The hybridization
buffer was prepared by dissolving 20 mM Tris HCl, 15 mM NaCl, 4 mM
KCl, 1 mM MgCl₂, and 1 mM CaCl₂ in molecular
biology grade water at pH 7.3; it was stored at 4 °C when it
is not in use. The buffer and working tools were DNase free.

Negri P., Choi J.Y., Jones C., Tompkins S.M., Tripp R.A, & Dluhy R.A. (2014). Identification of Virulence Determinants in Influenza Viruses. Analytical Chemistry, 86(14), 6911-6917.

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Nucleic Acid Purification and Synthesis

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Trizma (tris) base (2-amino-2-hydroxymethyl-1,3-propane-diol), sodium chloride (NaCl), magnesium chloride (Mgcl₂), Tris-2-carboxyethyl-phosphine (TCEP), 6-mercapto-1-hexanol (99%) (Sigma Aldrich), 1x Tris-EDTA, spermidine (99%) (Sigma-Aldrich), 200 proof ethanol, Rnase away (Thermo Scientific), Guanidine hydrochloride solution,8 M, pH 8.5, buffered aqueous solution (Sigma-Aldrich), Water (For RNA Work) (DEPC Treated) (Thermo Fisher Scientific) were all used as received. The buffer solutions and spermidine stock were prepared using ultrapure water (Mili-Q Ultrapure Water Purification, Milipore, Billerica, MA, U.S.A). All buffer and stock solutions to be used with RNA were made in a hood and with water (For RNA Work) (Fisher Bioreagents) (DEPC Treated). DNA and RNA sequences (Integrated DNA Technologies, Coralville, IA, U.S.A) were purified and synthesized using dual HPLC. Peptide Nucleic Acid sequences (PNA Bio, Newbury Park, CA, U.S.A) were purified and synthesized using HPLC.

, & Sternberg S. (1992). On the London equations.. Proceedings of the National Academy of Sciences of the United States of America, 89(22), 10673-10675.

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MAX Phase Ti3AlC2 Characterization

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Ti₃AlC₂ MAX was acquired from Aladdin (Shanghai, China). Tris (hydroxymethyl)aminomethane (Tris), and 6-Mercapto-1-hexanol (MCH) were acquired from Sigma. Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) and chloroauric acid (HAuCl₄·4H₂O, ≥99.9%) were ordered from J&K scientific. Hydrofluoric acid was acquired from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). 0.1 M pH 7.4 Tris-HCl buffer (100 mM NaCl and 20 mM MgCl₂) was used for electrochemical measurement, and 10 mM pH 7.4 Tris-HCl buffer was used as washing buffer. All DNA strands were ordered from Sangon Biotech Co., Ltd. (Shanghai, China), and the DNA sequences were provided in Table 1. 18.2 MΩ cm ultrapure water was used in whole experiments.

Yu X., Bai S, & Wang L. (2023). In situ reduction of gold nanoparticles-decorated MXenes-based electrochemical sensing platform for KRAS gene detection. Frontiers in Bioengineering and Biotechnology, 11, 1176046.

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Plasmonic Biosensor Platform for HMGB1 Detection

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All chemical reagents are analytical-graded reagents. Hydrogen tetrachloroaurate trihydrate (HAuCl₄), Phosphate buffered saline (PBS buffer), trisodium citrate solution (C₆H₅Na₃O₇, ≥99%), (3-Mercaptopropyl)methyldimethoxysilane (MPDMS, >95%), cystamine dihydrochloride (cystamine), dextran 70 (MW ≈ 70,000), sodium periodate (NaIO₄), 11-mercaptoundecanoic acid (MUA; ≥95%), 6-mercapto-1-hexanol (MCH; ≥97%), 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (NHS), ethanolamine (EA), mouse IgG, streptavidin, monoclonal anti-HMGB1 antibody, and high mobility group box 1 (HMGB1) protein were from Sigma-Aldrich (St. Louis, MO, USA). Sulfuric acid (H₂SO₄; ≥98%), hydrogen peroxide (H₂O₂), ethanol, and acetate buffer were purchased from Fluka (Buchs, Switzerland). Ultrapure deionized water (18.2 MΩ·cm⁻¹, Milli-Q pure water purification system, Millipore Ltd., Burlington, MA, USA) was used for preparing solutions. The optical fiber probe was multimode plastic-clad silica optical fiber (model F-MBC, Newport), with core and cladding diameters of 400 and 430 μm, respectively, bought from Instant NanoBiosensors Co., Ltd. (Taipei, Taiwan). Sensing chips (poly (methyl methacrylate) (PMMA) plates) were prepared using a CO₂ laser engraving machine (New Taipei, Taiwan).

Chiang C.Y., Chen C.H, & Wu C.W. (2023). Fiber Optic Localized Surface Plasmon Resonance Sensor Based on Carboxymethylated Dextran Modified Gold Nanoparticles Surface for High Mobility Group Box 1 (HMGB1) Analysis. Biosensors, 13(5), 522.

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Oligonucleotide Synthesis and Preparation

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All HPLC-purified oligonucleotides were synthesized by Sangon Inc. (Shanghai, China), and the base sequences were listed in Table S1. All oligonucleotides were dissolved in hybridization buffer (pH 7.4) containing 30 mM sodium phosphate, 450 mM NaCl, 3 mM EDTA, and 0.25% Triton 100, and stored at −20 °C for further measurement. 6-Mercapto-1-hexanol (MCH) and salmon sperm DNA were purchased from Sigma-Aldrich (St Louis, MO, USA). RNA extraction Kit was purchased from Amoy Diagnostics (Xiamen, China). RT-PCR Kit was purchased from Yqbiomed (Shanghai, China). All other reagents were of analytical grade and without further purification. Ultrapure water from a Millipore water purification system (≥18 MΩ⋅cm, Milli-Q, Millipore) was used in all experiments.

Guo B., Cheng W., Xu Y., Zhou X., Li X., Ding X, & Ding S. (2017). A simple surface plasmon resonance biosensor for detection of PML/RARα based on heterogeneous fusion gene-triggered nonlinear hybridization chain reaction. Scientific Reports, 7, 14037.

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