On P73 or 93, animals were again injected i.p. with their assigned training dose, and left undisturbed in their homecage until they were euthanized via decapitation for assessment of BECs 30 min post-injection. Trunk blood and brains were collected and maintained at −80 °C until analysis. Blood ethanol concentrations (BEC) were assessed via headspace gas chromatography using a Hewlett Packard (HP) 5890 series II Gas Chromatograph (GC) (Wilmington, DE) and procedures in standard use in our laboratory (e.g. see Willey et al., 2012 (link)).
Hp 5890 series 2 gas chromatograph
Manufactured by Hewlett-Packard
Sourced in Germany, United States
The HP 5890 series II Gas Chromatograph is a laboratory instrument designed for the analysis and separation of complex chemical mixtures. It functions by utilizing a carrier gas to transport the sample through a heated column, where the individual components are separated based on their physical and chemical properties. The separated components are then detected and quantified by the instrument's detection system.
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Lab products found in correlation
13 protocols using hp 5890 series 2 gas chromatograph
1
Ethanol Concentration Analysis Protocol
2
Ethanol Consumption and Blood Sampling
3
Measuring Maternal Blood Ethanol Levels
4
Chronic Intermittent Ethanol Exposure in Rats
5
GC-FID Analysis of Fatty Acids in Tissues
6
Blood Ethanol Concentration Measurement
7
Digesta pH and SCFA Analysis
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Digesta pH was measured by a WTW pH/340 pH-meter (WTW GmbH, Weilheim, Germany) and SCFA analysis was performed using the HP 5890 Series II gas chromatograph (Hewlett-Packard, Waldbronn, Germany) with isocaproic acid as the internal standard [9 ].
8
Quantitative Analysis of Fruit Odor
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For quantitative analyses, 0.1 μg of octadecane was added as an internal standard to each of the eluted fruit odor samples collected by dynamic headspace adsorption (see above). All samples were analyzed with an HP5890 Series II gas chromatograph (Hewlett-Packard, Palo Alto, CA, USA), equipped with a DB5 capillary column (30 m × 0.25 mm i.d.) that used hydrogen as the carrier gas (2 ml min−1 constant flow). One microliter of each sample was injected splitless at 40°C. After 1 min, the split valve was opened and the temperature increased by 4°C min−1 until reaching a temperature of 300°C. GC/MS analyses were carried out on an HP 6890 Series GC connected to an HP 5973 mass selective detector (Hewlett-Packard) fitted with a BPX5 fused-silica column (25 m, 0.22 mm i.d., 0.25 μm film thick, SGE). Mass spectra (70 eV) were recorded in full scan mode. Retention indices were calculated from a homolog series of n-alkanes. Structural assignments were based on comparison of analytical data obtained with natural products and data reported in the literature [6 (link), 26 (link), 34 ], and those of synthetic reference compounds. Structures of identified candidate compounds were verified by co-injection.
9
GC-MS Analysis of Volatile Compounds
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A HP 5890 Series II gas chromatograph (Hewlett-Packard, Heilbronn, Germany) was connected to a MAT 95 sector field mass spectrometer (Finnigan, Bremen, Germany). The fused silica column used was either a DB-FFAP, 30 m × 0.25 mm i.d., 0.25 µm film or a DB-5, 30 m × 0.25 mm i.d., 0.25 µm film (both Agilent). Helium was used as carrier gas at 1.9 mL/min constant flow. Further GC conditions were as described in the GC-O/FID section. Mass spectra were recorded either in EI mode (70 eV) with a scan range of m/z 35-300 or in CI mode (150 eV) with isobutane as reagent gas and a scan range of m/z 85-350. The evaluation was performed using Xcalibur software (Thermo Scientific, Dreieich, Germany).
10
GC-MS Analysis of Volatile Compounds
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A HP 5890 Series II gas chromatograph (Hewlett-Packard, Heilbronn, Germany) was connected to a MAT 95 sector field mass spectrometer (Finnigan, Bremen, Germany). The fused silica column used was either a DB-FFAP, 30 m × 0.25 mm i.d., 0.25 µm film or a DB-5, 30 m × 0.25 mm i.d., 0.25 µm film (both Agilent). Helium was used as carrier gas at 1.9 mL/min constant flow. Further GC conditions were as described in the GC-O/FID section. Mass spectra were recorded either in EI mode (70 eV) with a scan range of m/z 35-300 or in CI mode (150 eV) with isobutane as reagent gas and a scan range of m/z 85-350. The evaluation was performed using Xcalibur software (Thermo Scientific, Dreieich, Germany).
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