FT-IR spectra of the PU elastomer were analyzed to identify the functional group of each PU sample by using an FT-IR spectrophotometer (model Perkin Elmer Spectrum 400 FT-IR, PerkinElmer, Inc., Waltham, MA, USA) with the attenuated total reflectance (ATR) technique at a wave number that ranges from 4000 to 500 cm−1.
Spectrum 400 ft ir
Manufactured by PerkinElmer
Sourced in United States
The Spectrum 400 FT-IR is a Fourier Transform Infrared Spectrometer manufactured by PerkinElmer. It is designed to analyze the molecular composition of samples by detecting the absorption of infrared radiation. The instrument operates by passing infrared light through the sample and measuring the resulting spectrum, which can be used to identify and quantify the chemical components present.
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Lab products found in correlation
Cary 50 uv vis spectrophotometer, by Agilent Technologies (1 mentions)
Sd2000, by OceanOptics (1 mentions)
Jsm 6700f, by JEOL (1 mentions)
Merlin, by Zeiss (1 mentions)
Agilent hp 5ms capillary column, by Agilent Technologies (1 mentions)
Ft nir spectrometer, by PerkinElmer (1 mentions)
Series 200, by PerkinElmer (1 mentions)
10 protocols using spectrum 400 ft ir
1
FT-IR Analysis of PU Elastomer
2
Comprehensive Spectroscopic Analysis of Porous Silica Nanospheres
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1H and 13C Nuclear Magnetic Resonance (NMR) [1-dimensional (1D) and 2-dimensional (2D)] spectra were recorded on a Bruker/AVANCE III 600 MHz Fourier Transform Nuclear Magnetic Resonance 400 MHz cryoprobe spectrometer using tetramethyl silane (TMS) as an internal standard and deuterated dimethyl sulfoxide (DMSO-d6) solvent. Fourier transform-infrared spectroscopy (FTIR) spectra were captured on the Perkin Elmer Spectrum 400 FT-IR in the wavenumber range of 4000–400 cm−1. Mass spectra were acquired from BRUKER MicroTof-Q with DIONEX Ultimate 3000 LC. Differential Scanning Calorimeter (DSC, DSC882e Mettler Teledo) was used to measure the metal salphen complex melting temperature. The binding interaction between metal complex and double-stranded DNA (dsDNA) was carried out by spectrophotometric titration method using Varian Cary® 50 UV-Vis Spectrophotometer. The size distribution and particle morphology of the as-synthesized porous silica nanospheres (PSiNs) were examined by field emission scanning electron microscopy (FESEM, JEOL Co. Model JSM-6700F, Japan). A fiber optic reflectance spectrometer (SD 2000 Ocean Optics) was employed to record the reflectance intensity of the DNA biosensor in the wavelength range of 200–1099 nm.
3
FT-IR Spectroscopy of Sample
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FT-IR spectra were obtained by using a PerkinElmer FT-IR spectrometer (PerkinElmer Spectrum 400 FT-IR, Waltham, MA, USA) in the range of 500–4000 cm−1.
4
FTIR Analysis of Oxidized Carbohydrates
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Fourier transform infrared (FTIR) spectra of lyophilized samples and solid coating films were recorded using a PerkinElmer Spectrum 400 FTIR spectrometer equipped with an ATR unit. The signal intensity is given as absorbance. Calibration was done using 3-keto-α-methyl-d -glucopyranoside (100% oxidation degree) as well as native α-methyl-d -glucopyranoside and 6 kDa dextran (0% oxidation degree).
5
Sebum Fatty Acid Composition
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Forehead sebum was collected on absorbent paper, and attenuated total reflectance–Fourier transform infrared spectroscopy spectra were acquired in the 4,000–670 per cm range on a Perkin Frontier spectrometer (Spectrum 400 FT-IR, Perkin Elmer, Waltham, MA). The ratio of the peak areas of FFA (1,710 cm–1) and TGs (1,740 per cm) was calculated.
6
Characterization of CNC-rGO Nanocomposite
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The characterization of the individual material and nanocomposite was recorded using a field-emission scanning electron microscope (FESEM; Merlin, Zeiss, Germany). Fourier-transform infrared (FTIR) spectra were recorded using a Perkin Elmer Spectrum 400 FT-IR. Electrochemical characterization was carried out using cyclic voltammetry (CV) and differential pulse voltammetry (DPV), while electrochemical impedance spectroscopy (EIS) was performed using Autolab PGSTAT 12 Potentiostat (NOVA 1.10 software, Utrecht, The Netherlands). A conventional three-electrode system was used in which the screen-printed electrode (SPE) (Scrint Technology (M) Sdn. Bhd; Kedah, Malaysia) modified with CNC–rGO was employed as the working electrode, with the glassy carbon electrode as the counter electrode and Ag/AgCl as the reference electrode.
7
Characterization of Synthesized Flavor Esters
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Synthesized flavor esters were characterized using nondestructive FTIR-ATR performed on Perkin-Elmer Spectrum 400 FT-IR and FT-NIR Spectrometer (Perkin–Elmer Inc., Wellesley, MA, USA), equipped with PIKE GladiATR™ hovering monolithic diamond ATR accessory (Pike technologies Inc. USA). Briefly, aliquot sample (100 µL) of the crude extract was placed on the monolithic diamond ATR probe against the diamond crystal head. Thereafter, the samples were scanned at room temperature (25 °C) over a range of 4000–400 cm−1 as reported in the literature (Gumel et al. 2014 (link)).
Proton (1H) NMR analyses were recorded on JEOL JNM-GSX 270 FT-NMR (JOEL Ltd., Tokyo, Japan) machine at 250 MHz. About 1 µL of the crude ester was dissolved in deuterated chloroform (2 mL) containing tetramethylsilane (TMS) as the internal standard reference. The dissolved mixture was then filtered into an NMR tube using a glass syringe equipped with a 0.22 µm PTFE disposable filter (11807-25, Sartorius Stedim, Germany).
GC-MSMS analysis was carried out using Agilent triple quadrupole 7000B GCMSMS (Agilent, USA) equipped with GCMS triple axis detector carrying Agilent HP-5 ms capillary column (30 m long, 0.25 mm internal diameter, and 0.25 µm film thickness). The GC-MSMS injection and ramping method were conducted according to the previously reported literature (Gumel et al. 2013a (link), b (link), c (link)).
Proton (1H) NMR analyses were recorded on JEOL JNM-GSX 270 FT-NMR (JOEL Ltd., Tokyo, Japan) machine at 250 MHz. About 1 µL of the crude ester was dissolved in deuterated chloroform (2 mL) containing tetramethylsilane (TMS) as the internal standard reference. The dissolved mixture was then filtered into an NMR tube using a glass syringe equipped with a 0.22 µm PTFE disposable filter (11807-25, Sartorius Stedim, Germany).
GC-MSMS analysis was carried out using Agilent triple quadrupole 7000B GCMSMS (Agilent, USA) equipped with GCMS triple axis detector carrying Agilent HP-5 ms capillary column (30 m long, 0.25 mm internal diameter, and 0.25 µm film thickness). The GC-MSMS injection and ramping method were conducted according to the previously reported literature (Gumel et al. 2013a (link), b (link), c (link)).
8
Phytochemical Characterization of Chromolaena sp.
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The extraction process of pre prepared Chromolaena sp. was performed by using ultrasound (Q Soniqa) working at 60 amplitude and input power of 40-120 W. HPLC analysis was performed using Perkin Elmer series 200 by using HPLC-DAD (diode array detector). The separation achieved by a reverse phase C-18 column. Gradient elution of mobile phase that comprised a mixture of two solvents: 1% Acetic acid in water and methanol. The gradient program was started with 30, 60 and 90% of B at 2, 4, and 6 minute respectively. The wavelengths detection was read at 250 nm and 360 nm using a flow rate of 1.2 mL/min and sample injection volume of 50 μl. The stock solution of Gallic Acid was prepared and further diluted with the mobile phase solvent for standard curve. FTIR analysis was studied by analysing the prepared standard of gallic acid then followed by the sample. FTIR used in this study was the ATR model (Spectrum 400 FT-IR, Perkin Elmer). The wavenumber range was set between 4000-650 cm-1. Two drops of samples was placed on the surface of the sample holder to be analysed. The analysing procedure for sample was same as standard.
9
Gelatin-Glycerol-CMC Film Characterization
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Infrared spectra of the films were measured using FTIR spectrometer (Perkin Elmer Spectrum 400 FT-IR/FT-NIR & Spotlight 400 Imaging System). The sample scanning frequencies were in range of 4000 to 650 with spectra resolution of 4 cm -1 . The interactions among gelatin, glycerol, and CMC were determined through spectra obtained. Measurements were performed at room temperature and data were collected in triplicate. The functional group were identified by software and assigned according to the literature values.
10
Biofilm Characterization on RO Membranes
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Qualitative analysis of biofilm formed on RO membranes was done through FTIR spectroscopy (Perkin Elmer 400 FTIR instrument). RO membranes were transferred to sterile Petri dishes and subjected to oven drying for 2 h at 35 C. The membranes were then subsequently subjected to FTIR analysis [Spectrum 400 FTIR from PerkinElmer using Universal Attenuated Total Reflectance (UATR)] and spectra of 4,000-400 cm À1 were obtained.
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