Epz 6438

DMEM/F12K, trypsin, penicillin and streptomycin were purchased from Life technology. Sorafenib was purchased from Sigma (St. Louis, MO, USA). The EZH2 inhibitor EPZ‐6438 was obtained from Adooq Bioscience (Shanghai, China). Antibodies against RAF and VEGFP were brought from Abcam (Cambridge, MA, USA). The anti‐GAPDH and anti‐H3 antibodies were obtained from Sigma. Antibodies used to detect the protein levels of EZH2, H3K27me3 and H3K27Ac were purchased from Abcam. The secondary antibodies for Western blot were purchased from Jackson ImmunoReseach (West Grove, PA).

Pfeiffer were obtained from ATCC. HEK293 cells were obtained from Thermo Scientific. Pfeiffer cells, were cultured in RPMI 1460 (Life Technologies, 22400105). HEK293 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Life Technologies, 11965118). Culture media were supplemented with 10% of FBS (Life Technologies, 26140129).
Antibodies for immuno-blotting: EZH2 (CST, #5246), Histone H3 (CST, #4499), Histone H3 Lysine 27 Methyl 2(CST, #9728), Histone H3 Lysine 27 Methyl 3(CST, #9733). Antibody Histone H3 Lysine 27 Methyl 1 (EMD Millipore, #07–448). Anti-rabbit secondary antibody stored in 50% glycerol (Jackson Immunoresearch Laboratories, Inc., 205–035-108). GSK 126 and EPZ-6438 were purchased from AdooQ and Xcess Biosciences, respectively. Compounds were dissolved to 10 mM in dimethyl sulfoxide (DMSO).