The mother’s socio-demographic and clinical data were collected using a structured questionnaire based on interviews that was created in English and translated to the local languages. Maternal Mid upper arm circumference (MUAC) was measured using the standard method during the interview. The weight and sex of the newborn baby were determined by midwives at birth. Maternal Hgb was determined with a portable hemoglobinometer, the HemoCue Hgb analyzer (HemoCueHb 201+, Sweden) according to standard guidelines prior to the birth of the newborn. Two milliliters (2mL) of umbilical cord blood sample was collected. During the sampling, the umbilical cord was clamped 1–3 minutes after birth as recommended by the WHO.20 The collected sample was immediately poured into a test tube with ethylenediaminetetraacetic acid (EDTA) and gently mixed to prevent blood clotting. The collected sample was then taken to the hospital’s medical laboratory unit for Complete Blood Count (CBC) analysis using automated whole blood hematological analyzer (Mindray BC-5150). Two experienced laboratory technicians carried out the CBC in strict compliance with the standard operating procedures. In this study, newborn anemia is defined as Hgb <13.5g/dL (Adopted from study conducted in Ethiopia) and maternal anemia as Hgb <11g/dL.2 (link),21
Bc 5150
Manufactured by Mindray
Sourced in China
The BC-5150 is a compact and reliable hematology analyzer designed for small to medium-sized clinical laboratories. It provides accurate and efficient analysis of a wide range of complete blood count (CBC) and related parameters.
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9 protocols using bc 5150
1
Newborn and Maternal Anemia Evaluation
2
Antioxidant and Hematological Evaluation of Medicinal Plant Extracts
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Gallic acid, quercetin, 2,2-diphenyl-2-picrylhydrazyl, dichloromethane, and polyvinylpyrrolidone (PVP) were purchased from Sigma Chemical Co, USA, and were used in in vitro and in vivo experiments. All other chemicals and solvents were of analytical grade and were used without any purification.
A rotatory evaporator (Buchi, B-480, UK) was used for the efficient removal of solvents from plant extracts. A microplate reader (SpectraMax Plus, USA) was used in the determination of the total antioxidant activity of the selected medicinal plant extracts. A UV-visible, double-beam spectrophotometer (UV-1800 Shimadzu, USA) was used in the spectrophotometric bioassays. Hematological parameters were analyzed using an automated hematology analyzer (Mindray BC 5150, China). Tissue processor (Shandon, UK), microtome (Thermo Fisher, Germany), and microscope (Olympus CX 21, Japan) were used in the preparation and evaluation of hematoxylin and eosin- (H and E-) stained tissue sections.
A rotatory evaporator (Buchi, B-480, UK) was used for the efficient removal of solvents from plant extracts. A microplate reader (SpectraMax Plus, USA) was used in the determination of the total antioxidant activity of the selected medicinal plant extracts. A UV-visible, double-beam spectrophotometer (UV-1800 Shimadzu, USA) was used in the spectrophotometric bioassays. Hematological parameters were analyzed using an automated hematology analyzer (Mindray BC 5150, China). Tissue processor (Shandon, UK), microtome (Thermo Fisher, Germany), and microscope (Olympus CX 21, Japan) were used in the preparation and evaluation of hematoxylin and eosin- (H and E-) stained tissue sections.
3
Differential Blood Count Analysis
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A complete differential blood counting was performed using a hematology analyzer (Mindray BC-5150). The ratio of monocytes to lymphocytes (MLR) and neutrophils to lymphocytes (NLR) was determined and compared among the four different study groups.
4
Hematological Parameters Analysis Protocol
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On the 28th day, blood samples (4 mL) were collected through direct cardiac puncture under light ether vapor anesthesia to analyze the hematological parameters. A hematology analyzer, Mindray BC-5150, was used to calculate the hemoglobin (Hb) and total leucocyte count (TLC). The erythrocyte sedimentation rate (ESR) was measured by the Wintrobe method [41 (link)]. Rheumatoid factor (RhF) levels were detected and semi-quantified through the agglutination method by a commercially available agglutination kit (Antec Diagnostics, UK).
5
Hematological Profiling of Tribal Populations
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The socio-demographic details of the selected tribes were recorded using a standardized questionnaire. About 5 mL of intravenous blood samples were collected in an EDTA containing vacutainer from each of the eligible participants. The RBC indices were undertaken using an automated 5-part hematology analyzer (Mindray, BC-5150). The hemoglobin fractions were identified using the HPLC system (Bio-Rad, D-10) following standard protocols. HbA2 value is a main factor in determining the categorized Hb abnormalities along with RBC indices. An HbA2 level of >3.9% was used as a cut-off for diagnosis of β-thalassemia trait (βTT), < 2.0% was used for α-thalassemia trait (αTT), and borderline values were fixed within the range of 3.6-3.9% [12] (link).
6
Automated Blood Count Analysis
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The complete blood count was analyzed from a 2 ml probe with EDTA using the Mindray BC-5150 automatic laboratory analyzer (Shenzhen Mindray Bio-Medical Electronics Co., PRC). Then, the PRP sample was analyzed in the same fashion from the Eppendorf tube.
7
Evaluating Tumor Progression in Mice
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One day before the experiment, 20 µL of peripheral blood drawn from the tail vein were collected to evaluate hematological parameters. The next day, five groups of mice (n = 5) were inoculated intravenously (i.v.) by lateral tail-vein injection with 5 × 102, 5 × 103, 5 × 104, 5 × 105 or 1 × 106 of DA-3/ER-GM cells. All mice were monitored three times a week for body weight and signs of illness (hunched posture, ruffled fur, lethargy, and pale mucous membranes). Additionally, hematological parameters were analyzed with a blood count using the Mindray BC-5150 (Mindray Bio-Medical Electronics Co., Ltd., Shenzhen, China) and a peripheral blood smear Wright’s stained using an automated stainer (Aerospray Stainer, Wescor, Logan, UT, USA). Mice were euthanized by CO2 inhalation and necropsied immediately. Spleen, liver, lung and femur were immersion-fixed in 10% neutral buffered formalin, and stained with hematoxylin and eosin using standard protocols. Slides were reviewed by a board-certified pathologist, who confirmed the appearance of metastases and tissue changes.
8
Whole Blood PRP Preparation and Analysis
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First, the whole blood samples collected for EDTA were analyzed. The time between blood draw, PRP processing, extraction and activation did not exceed 1 h. All preparations were conducted in daylight and at room temperature. The whole blood count and blood cell composition of PRP samples were analyzed using an automatic laboratory analyzer Mindray BC-5150 (Shenzhen Mindray Bio-Medical Electronics Co., PRC) which needs 20 µL for each analysis. Immediately after PRP preparation, each sample was transferred into Eppendorf polypropylene tubes and then shaken gently for 30 s directly before analysis. Platelet capture efficiency (PCE) was calculated with the formula below, described previously by J. Magalon[23 (link)].
PCE (%) = [Volume of PRP obtained (mL) × platelet concentration in PRP (G/L)]/[Net volume of whole blood collected (mL) × platelet concentration in whole blood (G/L)].
PCE (%) = [Volume of PRP obtained (mL) × platelet concentration in PRP (G/L)]/[Net volume of whole blood collected (mL) × platelet concentration in whole blood (G/L)].
9
Mean Platelet Volume in Acute Pancreatitis
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MPV is a standard parameter included in the complete blood count panel for all patients at our hospital. It is measured using automatic hematological analyzers MINDRAY BC-5150 or BC-3000 plus, manufactured by Shenzhen Mindray Bio-Medical Electronics Co. The MPV measurement was routinely performed for all patients in the ICU and the Surgical Unit within the first 24 h of hospitalization. If more than one measurement of MPV was available during this time, the analysis included the highest deviating value Given the lack of a significant correlation between the MPV and the number of platelets among patients with AP within the first 24 h of hospitalization, the number of platelets was not included in the current study. A threshold value for MPV was established for the groups based on the severity of AP. Based on this threshold, two groups were defined, categorized as low and high MPV, with corresponding evaluations of key clinical characteristics and outcomes.
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