Foretinib (GSK1363089, EXEL2880, XL880; Chemietek), cabozantinib (XL184, BMS-907351; Chemietek), motesanib free base (AMG-706; Selleckchem), PF-04217903 (Selleckchem), barasertib (AZD1152-HQPA; Selleckchem), trametinib (GSK1120212; Chemietek), GSK1838705A (Selleckchem), PF-573228 (Selleckchem), linsitinib (OSI-906; Chemietek) and alisertib (MLN-8237; Selleckchem) were dissolved in DMSO (10 mM). Drug dilutions were made in DMSO as necessary.
Gsk1838705a
Manufactured by Selleck Chemicals
Sourced in United States
GSK1838705A is a laboratory reagent used in research and development applications. It is a small molecule inhibitor targeting the activity of the PI3K/Akt/mTOR signaling pathway. The core function of GSK1838705A is to modulate the activity of this signaling pathway in experimental cell-based and biochemical assays.
Automatically generated - may contain errors
Lab products found in correlation
Regorafenib, by Bayer (4 mentions)
Sorafenib, by Bayer (2 mentions)
Recombinant human igf 1, by Thermo Fisher Scientific (2 mentions)
Hepg2, by American Type Culture Collection (2 mentions)
Hep3b, by American Type Culture Collection (2 mentions)
Trametinib, by Chemietek (1 mentions)
Pf 04217903, by Selleck Chemicals (1 mentions)
Barasertib azd1152 hqpa, by Selleck Chemicals (1 mentions)
Pf 573228, by Selleck Chemicals (1 mentions)
Alisertib mln8237, by Selleck Chemicals (1 mentions)
Ramucirumab, by Eli Lilly (1 mentions)
Nvp adw742, by Selleck Chemicals (1 mentions)
Osi 906, by Selleck Chemicals (1 mentions)
Recombinant human egf, by Thermo Fisher Scientific (1 mentions)
Erlotinib hcl, by Selleck Chemicals (1 mentions)
7 protocols using gsk1838705a
1
High-Throughput Kinase Inhibitor Screening
2
Establishment of Human Hepatocellular Carcinoma Cell Lines
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PLC/PRF/5 and HepG2 human HCC cell lines were purchased from the National Institute of Biomedical Innovation JCRB Cell Bank (Osaka, Japan) and cultured with Dulbecco’s Modified Eagle’s Medium (DMEM). All cell culture supplements were purchased from Sigma-Aldrich (Milan, Italy).
Regorafenib and sorafenib were a gift from Bayer Corp (West Haven, CT, USA); ramucirumab was purchased from Eli Lilly (Utrecht, The Netherlands), GSK1838705A was purchased from Selleck Chemicals (Houston, TX, USA). Recombinant Insulin-like Growth Factor 1 (IGF1) was purchased from Calbiochem (San Diego, CA, USA) and recombinant Vascular Endothelial Growth Factor from ORF Genetics (Kopavogur, Iceland). The two cell lines were grown in monolayer culture with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin addition and incubated at 37 °C in a humidified atmosphere containing 5% CO2 in air.
Regorafenib and sorafenib were a gift from Bayer Corp (West Haven, CT, USA); ramucirumab was purchased from Eli Lilly (Utrecht, The Netherlands), GSK1838705A was purchased from Selleck Chemicals (Houston, TX, USA). Recombinant Insulin-like Growth Factor 1 (IGF1) was purchased from Calbiochem (San Diego, CA, USA) and recombinant Vascular Endothelial Growth Factor from ORF Genetics (Kopavogur, Iceland). The two cell lines were grown in monolayer culture with 10% fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin addition and incubated at 37 °C in a humidified atmosphere containing 5% CO2 in air.
3
Evaluation of IGF1R Inhibitors and Doxorubicin
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The IGF1R inhibitors OSI-906, NVP-ADW742 and GSK1838705A were purchased from Selleck Chemicals LCC (see Additional file 1 : Table S1 for properties) and dissolved in DMSO in a concentration of 10 mM. The IGF1R inhibitors were tested in concentrations up to 1 μM as it was demonstrated previously that higher concentrations lead to an aspecific toxic response [24 (link)]. Recombinant human IGF1 (PeproTech) was used in a concentration of 50 ng/ml [25 (link)]. Doxorubicin was obtained from the in-house hospital pharmacy in a 0.9 % NaCl solution, and used in a concentration range of 1–100 nM.
4
Regorafenib and IGF1 Inhibition in HCC
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Regorafenib was gifts from the Bayer Corp (West Haven, CT, USA), recombinant human IGF1 was purchased from Pepro-Tech (Rocky Hill, NJ, USA), GSK1838705A was purchased from Selleckchem (Houston, TX, USA).
Hep3B, HepG2 and PLC/PRF/5 human HCC cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). The culture medium was Dulbecco’s Modified Eagle’s Medium (DMEM). All cell culture components were purchased from Sigma- Aldrich (Milan, Italy).
Hep3B, HepG2 and PLC/PRF/5 human HCC cells were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA). The culture medium was Dulbecco’s Modified Eagle’s Medium (DMEM). All cell culture components were purchased from Sigma- Aldrich (Milan, Italy).
5
Evaluating HCC Cell Line Responses
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PLC/PRF/5, HepG2 and HLF human HCC cell lines were purchased from the National Institute of Biomedical Innovation JCRB Cell Bank (Osaka, Japan). The culture medium was Dulbecco’s Modified Eagle’s Medium (DMEM). All cell culture components were purchased from Sigma-Aldrich (Milan, Italy). Regorafenib was gifted from Bayer Corp (West Haven, CT USA ); vitamin K1 was purchased from International Medication Systems, Limited (So. El Monte, CA, USA), GSK1838705A and S1091 Linsitinib (OSI-906) from Selleckchem (Houston, TX, USA).
6
Anticancer Compound Screening in Human HCC Cells
7
GSK1838705A Inhibition of U87MG Glioma in Mice
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GSK1838705A was purchased from Selleck Chemicals LLC (Houston, TX, USA). The human U87MG glioma cell line was purchased from American Type Culture Collection (Rockville, MD, USA). The U87MG cells were maintained in Eagle's minimal essential medium (EMEM), supplemented with 10% heat-inactivated fetal bovine serum (FBS) and 100 U/ml penicillin-streptomycin in a humidified incubator at 37°C with 5% CO2. Athymic nude mice were purchased from Slack Company (Shanghai, China) and were handled in compliance with the Experimental Animal Care and Use Committee guidelines of Xinxiang Medical University (Xinxiang, Henan, China). A total of 18 female mice (age, 7–8 weeks; weight, 24–28 g) were housed in a specific-pathogen-free facility maintained between 20 and 25°C with a 12 h light/12 h dark cycle, and were allowed ad libitum access to food and water. The study was approved by the ethics committee of Xinxiang Medical University (Xinxiang, China).
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