F254 plate

Manufactured by Merck Group

Sourced in Germany, United States, India

F254 plates are thin-layer chromatography (TLC) plates designed for qualitative analysis and separation of compounds. They are coated with a fluorescent indicator that allows visualization of separated compounds under ultraviolet (UV) light. The plates provide a stable and reproducible platform for conducting TLC experiments.

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Lab products found in correlation

Nmr spectrometer, by Bruker (8 mentions) Ftir 8400s spectrophotometer, by Shimadzu (6 mentions) Amx 500 spectrometer, by Bruker (3 mentions) Jms 700 high resolution mass spectrophotometer, by JEOL (3 mentions) Linomat 5, by CAMAG (2 mentions) Av 300 spectrometer, by Bruker (2 mentions) Pe2400 seriesii chns o elemental analyzer, by PerkinElmer (2 mentions) P anisaldehyde h2so4 reagent, by Merck Group (2 mentions) 6 methoxy 3h 1 3 benzoxazol 2 one 6 mboa mboa, by Fujifilm (2 mentions) α glucosidase from rice, by Toyobo (2 mentions) β amylase, by Toyobo (2 mentions) β glucosidase, by Toyobo (2 mentions) Milli q system, by Merck Group (2 mentions) Twin trough glass chamber, by CAMAG (2 mentions) 1 3 dichloro 1 1 3 3 tetraisopropyldisiloxane, by Merck Group (2 mentions) 1 m tetra butylammonium fluoride tbaf solution in thf, by Merck Group (2 mentions) Lichroprep rp 18, by Merck Group (2 mentions) Mci gel chp 20p, by Mitsubishi (2 mentions) Linomat 5 applicator, by CAMAG (1 mentions) Micromass q tof, by Waters Corporation (1 mentions)

Spelling variants of this product

RP-18 F254S plates (53 citations) Precoated RP-18 F254s plates (5 citations) RP-18 F254s TLC plates (4 citations) Pre-coated silica gel 60 F254 TLC plates (3 citations) Silica gel 60 F254 precoated glass plates (3 citations) Silica gel 60 RP-18 F254S pre-coated plates (2 citations) 60 F254S plates (2 citations) Silica gel 60 RP-18 F254S plate (2 citations) TLC Silica gel F254 plates (2 citations) RP-18 F254 TLC plates (2 citations)

71 protocols using f254 plate

Photochemical Reactions and Characterization

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Photochemical reactions were carried out
in a quartz-lined photochemical reactor with a medium-pressure mercury
vapor lamp (Philips, 450 W) installed in a water-cooled immersion
well with a constant supply of water. The incident photon flux of
the irradiation system was 8.72107 einstein/min, according to ferrioxalate
actinometry.⁴⁴ On a PerkinElmer model spectrum
RXI, IR spectra were captured as KBr discs. TMS as an internal standard
and CDCl₃ as a solvent were used to record ¹H NMR and ¹³C NMR spectra on a Bruker Avance DRX-300 spectrometer.
A VG-ZAB-BEQ9 spectrometer was used to obtain high-resolution mass
spectra at a 70 eV ionization voltage. For analytical TLC, Merck silica
gel 60 F254 plates (0.2 mm thick) were used, whereas Merck silica
gel 60 F254 plates (0.2 mm thick) were used for column chromatography
(60–120 mesh). A PerkinElmer PE 2400 series II CHNS/O elemental
analyzer was used for the elemental analysis.

Gupta A., Zaheer M.R., Iqbal S., R.o.o.h.i., Ahmad A, & Alshammari M.B. (2022). Photodegradation and In Silico Molecular Docking Study of a Diuretic Drug: Clopamide. ACS Omega, 7(16), 13870-13877.

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Characterization of Ginger Extract by Advanced Chromatographic Techniques

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GE was analyzed by High Performance Thin Layer Chromatography (HPTLC). Samples (20 μL) were applied as bands on aluminum coated TLC silica gel 60 F-254 plates (Merck, Germany) using Camag Linomat 5 applicator. The plates were developed using a mobile phase consisting of toluene: ethyl acetate: chloroform: methanol (8:20:12:60% v/v). The plates were then scanned at 254 nm using Camag TLC scanner 3.
GE was further chromatographed using Buchi C615 flash chromatography system (Buchi Sepacore Record 1.0 software). Fractions were eluted using a gradient of chloroform: methanol (100:0–90:10). The fractions that were obtained were further purified by preparative TLC using silica gel GF₂₅₄ as the stationary phase and toluene: ethyl acetate: chloroform: methanol (8:20:12:60% v/v) as the mobile phase. The compounds which were isolated were characterized by Liquid Chromatography-Mass Spectrometry (LC-MS) by electro spray positive ionization technique using WATERS, Micromass Q-TOF with Waters Alliance 2795 micro separation module (Waters X-Terra C-18 column); Fourier Transform-Infrared spectroscopy (FT-IR) (JASCO FT/IR-4100 type A instrument) and ¹H-Nuclear Magnetic Resonance (¹H-NMR) (Bruker Advance II 400 NMR spectrometer with 1.3 Topsin software using deuterated chloroform as solvent).

Nair S.S., Gaikwad S.S., Kulkarni S.P, & Mukne A.P. (2017). Allium sativum Constituents Exhibit Anti-tubercular Activity In vitro and in RAW 264.7 Mouse Macrophage Cells Infected with Mycobacterium tuberculosis H37Rv. Pharmacognosy Magazine, 13(Suppl 2), S209-S215.

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TLC Profiling of Plant Extracts

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The comparative TLC chromatograms and fingerprint profiles were developed using pre-coated silica gel F₂₅₄ plates [E. Merck (India) Ltd., alumina base, and 0.2 mm thickness]. A large number of various combinations of solvent systems were used during the present studies and the best resolution was obtained in a solvent system of toluene:ethyl acetate:acetic acid (7.4:2.4:0.2). Extracts were applied as bands using Camag Linomat 5 available in the laboratory. The running distance was kept at 8 cm and anisaldehyde sulphuric acid reagent was used as derivatizing agent followed by heating at 110 °C for 5 min or till the bands developed colour. TLC fingerprint profiles were recorded as images under UV at 254 and 366 nm before spray and under white light after derivatization on Camag Reprostar fitted with D × A 252 16 mm camera.

Jena A.K., Karan M, & Vasisht K. (2017). Plant parts substitution based approach as a viable conservation strategy for medicinal plants: A case study of Premna latifolia Roxb.. Journal of Ayurveda and Integrative Medicine, 8(2), 68-72.

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Synthesis and Characterization of Novel Organic Compounds

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Reagent-grade chemicals and solvents were purchased from commercial supplier and used after purification. Thin-layer chromatography (TLC) was performed on silica-gel F254 plates (Merck). Merck silica gel (60–120 mesh) was used for column chromatographic purification. All reactions were carried out in a nitrogen atmosphere. Melting points are uncorrected and were measured in open capillary tubes, using a Rolex melting-point apparatus. IR spectra were recorded as KBr pellets on a Perkin-Elmer RX 1 spectrometer and the wave numbers are reported in cm⁻¹. ¹H NMR and ¹³C NMR spectral data were recorded on Advance Bruker 400 spectrometer (¹H 400 MHz/¹³C 100 MHz) with DMSO-d₆ as solvent and tetramethylsilane (TMS) as internal standard and reported in δ (ppm). J values are in hertz (Hz). Mass spectra were determined by High Resolution Mass Specter (HRMS).

Ugwuja D.I., Okoro U.C., Soman S.S., Soni R., Okafor S.N, & Ugwu D.I. (2019). New peptide derived antimalaria and antimicrobial agents bearing sulphonamide moiety. Journal of Enzyme Inhibition and Medicinal Chemistry, 34(1), 1388-1399.

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Characterization of Synthesized Compounds

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Melting points were determined on a capillary point apparatus (Stuart SMP3) equipped with a digital thermometer. IR spectra (KBr) were recorded on a Shimadzu FT-IR 8400S spectrophotometer. Reactions were monitored using thin layer chromatography (TLC) on 0.2 mm silica gel F254 plates (Merck) utilizing various solvents for elution. The chemical structures of the synthesized compounds were characterized by nuclear magnetic resonance spectra (¹H-NMR, ¹³C-NMR) and determined on a Bruker NMR spectrometer (500 MHz, 125 MHz for ¹H and ¹³C, respectively). ¹³C-NMR spectra are fully decoupled. Chemical shifts were reported in parts per million (ppm) using the deuterated solvent peak or tetramethylsilane as an internal standard. Colorimetric enzyme inhibitory assays were performed in 96-well plates and the absorbance was recorded utilizing a microplate reader (Infinite F50, Tecan, Switzerland).

Youssef M.A., Panda S.S., El-Shiekh R.A., Shalaby E.M., Aboshouk D.R., Fayad W., Fawzy N.G, & Girgis A.S. (2020). Synthesis and molecular modeling studies of cholinesterase inhibitor dispiro[indoline-3,2′-pyrrolidine-3′,3′′-pyrrolidines]. RSC Advances, 10(37), 21830-21838.

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Synthesis and Characterization of FA Derivatives

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All reagents were purchased from commercial suppliers of Adamas Reagent Ltd. (Shanghai, China) in analytical reagent grade and were used directly without further purification. Flash chromatography was carried out with silica gel (200–300 mesh) which was supplied by Innochem Co., Ltd. (Beijing, China). Analytical TLC was performed on pre-coated silica gel F254 plates (0.25 mm; E. Merck), and the products were visualized by UV detection or treated with an ethanolic solution of p-anisaldehyde spray followed by heating. The derivatives of FA were characterized by ¹H NMR, ¹³C NMR, HRMS, and elemental analysis. The antibacterial activity was assayed by using a multi-model plate reader (Infinite 200).

Zheng W., Tu B., Zhang Z., Li J., Yan Z., Su K., Deng D., Sun Y., Wang X., Zhang B., Zhang K., Wong W.L., Wu P., Hong W.D, & Ang S. (2023). Ligand and structure-based approaches for the exploration of structure–activity relationships of fusidic acid derivatives as antibacterial agents. Frontiers in Chemistry, 10, 1094841.

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Phytochemical Analysis of Extracts

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The TLC profile was established on silica gel F254 plates (Merck, Darmstadt, Germany) using butanol/acetic acid/water (65:25:10) as the mobile phase. UV light at 254 nm and 366 nm, visible light anisaldehyde and heat were used to detect spots. The ultraviolet spectra of the extracts were recorded from 200 nm to 400 nm on a SPECORD-200 plus UV-Vis spectrophotometer (Analytik Jena, Jena, Germany). For IR spectroscopy, a KBr tablet containing the dried extract was prepared and the IR spectrum was recorded from 4000 to 700 cm⁻¹ on a FT/IR-460 spectroscope (Jasco plus, Oklahoma, OK, USA).

Gutiérrez Gaitén Y.I., Felipe González A., Scull Lizama R., Núñez Cairo C.R., Álvarez Hernández G.L., Díaz Masó V., Noa Rodríguez A.C., Herrera Isidrón J.A., Pieters L., Foubert K, & Herrera Isidrón L. (2023). Pharmacognostic Study, Diuretic Activity and Acute Oral Toxicity of the Leaves of Xiphidium caeruleum Aubl. Collected in Two Different Phenological Stages. Plants, 12(6), 1268.

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Synthesis and Characterization of Novel Compounds

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All solvents and chemicals were purchased from Merck and Aldrich. All reactions and the purity of the products were monitored by thin-layer chromatography (TLC) using aluminum plates coated with silica gel F254 plates (Merck) using ethyl acetate and n-hexane as eluents. UV light with a wavelength of 254 nm was used for the detection of products. By using an Electro thermal 9100, melting points were determined in open capillaries. IR spectra were run on a 400s Shimadzu FTIR Spectrophotometer (as KBr pellets). ¹H and ¹³C NMR spectra were recorded on a 500 MHz Bruker Avance DRX Spectrometer instrument using TMS as an internal standard and CDCl₃, DMSO-d6 as a solvent. The XRD patterns were obtained on an X-ray diffractometer (Holland, Philips Xpert, Co K, radiation, λ = 0.178897 nm). A Field Emission Scanning Electron Microscope (FE-SEM) with 15 KV, Mira3, Tescan), Thermal Gravimetric Analysis (TGA D-32609 from Hullhorst), and Transmission electron microscope (TEM, Philips –CM120, 100 KV) were used. An ultrasonic probe watt ultrasonic homogenizer 400 from Topsonics Co was used in room temperature for optimization of the reaction.

Banazadeh M., Amirnejat S, & Javanshir S. (2020). Synthesis, Characterization, and Catalytic Properties of Magnetic Fe3O4@FU: A Heterogeneous Nanostructured Mesoporous Bio-Based Catalyst for the Synthesis of Imidazole Derivatives. Frontiers in Chemistry, 8, 596029.

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Organic Synthesis and Characterization

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All of the reagents and solvents were purchased from Aladdin (Shanghai, China) or Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China), and were used as received. Melting points were determined in open capillary tubes and are uncorrected. Reaction courses were monitored by thin-layer chromatography on silica gel-precoated F₂₅₄ plates (Merck, Darmstadt, Germany). Developed plates were examined with UV lamps (254 nm). Nuclear magnetic resonance spectroscopy was performed on an AV-300 spectrometer (Bruker, Zurich, Switzerland) operating at 300 MHz for ¹H and 75 MHz for ¹³C and using DMSO-d₆ as solvent and tetramethylsilane as the internal standard. An MALDI-TOF/TOF mass spectrometer (Bruker Daltonik, Bremen, Germany) was used to measure the high resolution mass spectroscopy.

Song M.X., Wang Z.Y., He S.H., Yu S.W., Chen S.L., Guo D.F., Zhao W.H, & Deng X.Q. (2018). Synthesis and Evaluation of the Anticonvulsant Activities of 4-(2-(Alkylthio)benzo[d]oxazol-5-yl)-2,4-dihydro-3H-1,2,4-triazol-3-ones. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(4), 756.

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Thin Layer Chromatography of E. cicutarium Extracts

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E. cicutarium extracts (Podvinje-M and Podvinje-W) were dissolved (c = 5 µg/µL) and applied on TLC silica gel F₂₅₄ plates (20 × 20 cm; thickness 0.25 mm; Merck, Germany) in 8 mm wide bands using an automatic TLC sampler (ATS4, CAMAG, Muttenz, Switzerland). An acetonitrile/water/formic acid = 30:8:2 (V/V/V) mixture was used as a mobile phase. Plates developed for visualization were dried using a hair dryer, in a stream of cold air for 15 to 20 min. Visualization was performed using NSR (1% in methanol, m/V; Naturstoff reagent A; diphenylboric acid 2-aminoethyl ester; Sigma-Aldrich, St. Louis, MO 63118, USA) at 366 nm and 254 nm (Figure 5). Plates developed in parallel for bioautography under the same conditions were left to dry additionally overnight at room temperature in the dark.

Ljoljić Bilić V., Gašić U.M., Milojković-Opsenica D., Rimac H., Vuković Rodriguez J., Vlainić J., Brlek-Gorski D, & Kosalec I. (2022). Antibacterial Fractions from Erodium cicutarium Exposed—Clinical Strains of Staphylococcus aureus in Focus. Antibiotics, 11(4), 492.

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