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Topotecan hcl

Manufactured by Selleck Chemicals
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Topotecan HCl is a chemical compound used in laboratory settings. It is a synthetic derivative of the natural product camptothecin, which is extracted from the bark of the Camptotheca acuminata tree. Topotecan HCl functions as a topoisomerase I inhibitor, a class of anticancer agents that target and disrupt the activity of the topoisomerase I enzyme, which is involved in the regulation of DNA structure and function.

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Lab products found in correlation

Idarubicin hcl, by Selleck Chemicals (2 mentions) Pf 477736, by Selleck Chemicals (1 mentions) Hek293t, by American Type Culture Collection (1 mentions) Hs578t, by American Type Culture Collection (1 mentions) Bt549, by American Type Culture Collection (1 mentions) Hcc38, by American Type Culture Collection (1 mentions) Hcc1419, by American Type Culture Collection (1 mentions) Mda mb 231, by American Type Culture Collection (1 mentions) Mcf 7, by American Type Culture Collection (1 mentions) Sw480, by American Type Culture Collection (1 mentions) Sw620, by American Type Culture Collection (1 mentions) Ethidium bromide, by Merck Group (1 mentions) Aphidicolin, by Merck Group (1 mentions) Mv4 11, by American Type Culture Collection (1 mentions) Du4475, by American Type Culture Collection (1 mentions) Hcc2218, by American Type Culture Collection (1 mentions) Hcc1187, by American Type Culture Collection (1 mentions) Neocarzinostatin, by Merck Group (1 mentions) Camptothecin, by Selleck Chemicals (1 mentions) Ym155, by Selleck Chemicals (1 mentions)

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Topotecan (17 citations)

4 protocols using topotecan hcl

1

Preparation of Topotecan and PF477736 Stock Solutions

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Stock solutions of Topotecan HCl (Selleck, S1231) and PF477736 (Selleck, S2904) were prepared in DMSO and aliquots were stored at −80°C.
Kim M.K., James J, & Annunziata C.M. (2015). Topotecan synergizes with CHEK1 (CHK1) inhibitor to induce apoptosis in ovarian cancer cells. BMC Cancer, 15, 196.
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2

Cell Lines and Reagents for Cytotoxicity Assays

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A549, HUH7, SW480, SW620, HCC38, HCC1187, HS578T, MDA-MB-231, DU4475, MV4-11, HCC2218, BT-549, MCF-7, HCC1419, PC-3 and HEK 293T cell lines were obtained from the American Type Culture Collection and were cultured in the suggested media. YM155, idarubicin-HCl, topotecan-HCl and camptothecin were purchased from Selleck Chemicals (Houston, TX, USA) and nilotinib was purchased from LC Laboratories (Woburn, MA, USA). Aphidicolin, Neocarzinostatin and Ethidium Bromide were purchased at Sigma Aldrich. Antibodies used in these study: a-Flag (Sigma), a-Actin (Cytoskeleton), a-Kap1, a-phospho Kap1 (both Bethyl Laboratories), a-gH2AX, (Millipore).
Winter G.E., Radic B., Mayor-Ruiz C., Blomen V.A., Trefzer C., Kandasamy R.K., Huber K.V., Gridling M., Chen D., Klampfl T., Kralovics R., Kubicek S., Fernandez-Capetillo O., Brummelkamp T.R, & Superti-Furga G. (2014). The solute carrier SLC35F2 enables YM155-mediated DNA damage toxicity. Nature chemical biology, 10(9), 768-773.
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3

Antibody Procurement and Reagent Catalog

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Antibodies against Flag (catalog no. F1804) and hemagglutinin (HA) (catalog no. H6908) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Antibodies against IFNAR1 (catalog no. ab45172) and NSE1 (catalog no. ab139689) were purchased from Abcam (Cambridge, MA, USA). Antibody against IRAK1 (catalog no. 4504S) was purchased from Cell Signaling Technology (CST) (Beverly, MA, USA). Antibodies against GAPDH (catalog no. 60004-1-Ig), GFP (catalog no. 66002-1-Ig), GST (catalog no. 66001-1-Ig), SMC5 (catalog no. 14178-1-AP), SMC6 (catalog no. 14465-1-AP), and PJA1 (catalog no. 17687-1-AP) were purchased from Proteintech Group (Wuhan, Hubei, China). Antibody against NSMCE4A (catalog no. AP9909a) was purchased from Abgent (San Diego, CA, USA).
Hepatitis B virus antigen detection ELISA kits were purchased from Ke Hua Bio Engineering (Shanghai, China). The inhibitors Braco19 and MG132 were purchased from Sigma-Aldrich. 3-Deazaneplanocin A (DZNeP), 5-aza-2ʹ-deoxycytidine (5′-Aza), GSK343, idarubicin HCl, and topotecan HCl were purchased from Selleck Chemicals (Houston, TX, USA).
Xu W., Ma C., Zhang Q., Zhao R., Hu D., Zhang X., Chen J., Liu F., Wu K., Liu Y, & Wu J. (2018). PJA1 Coordinates with the SMC5/6 Complex To Restrict DNA Viruses and Episomal Genes in an Interferon-Independent Manner. Journal of Virology, 92(22), e00825-18.
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4

Estrone Sulfate Membrane Protein Assay

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[3H]-labeled estrone sulfate (ES) was purchased from PerkinElmer (Waltham, MA, USA). Membrane-impermeable biotinylation reagent Sulfo-NHS-SS-biotin, streptavidin agarose resin, protein G agarose resin, an anti-mouse HRP-linked antibody, and an anti-rabbit HRP-linked antibody were purchased from Thermofisher Scientific (Waltham, MA, USA). A mouse anti-myc antibody (clone 9E10) was bought from Roche (Indianapolis, IN, USA). Rabbit anti-SUMO2/3, anti-Ubc9, and anti-SENP2 antibodies were purchased from Abcam (Cambridge, MA, USA). Topotecan HCL, ginkgolic acid (GA), and 2-D08 were obtained from Selleck Chemicals (Houston, TX, USA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Yu Z, & You G. (2024). Topotecan and Ginkgolic Acid Inhibit the Expression and Transport Activity of Human Organic Anion Transporter 3 by Suppressing SUMOylation of the Transporter. Pharmaceutics, 16(5), 638.
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