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Colorimetric tunel staining kit

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The Colorimetric TUNEL staining kit is a laboratory product that detects DNA fragmentation, a hallmark of apoptosis or programmed cell death. The kit utilizes a terminal deoxynucleotidyl transferase (TdT) enzyme to incorporate labeled nucleotides into the free 3'-hydroxyl ends of DNA fragments, which can then be visualized using a colorimetric detection system.

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TUNEL kit (56 citations) TUNEL staining (24 citations) TUNEL staining kit (16 citations)

2 protocols using colorimetric tunel staining kit

1

Zebrafish Embryo Proliferation and Apoptosis

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Zebrafish embryos were chemically treated between 48–72 hpf and fixed at 72 hpf. For proliferation analysis, embryos were permeablized and stained with primary antibody against phospho-Histone H3, and FITC-conjugated secondary antibody. Embryos were imaged and phospho-H3 positive cells in the CHT were manually counted. Secondary antibody-only control showed no non-specific staining. For apoptosis analysis, embryos were stained using the colorimetric TUNEL staining kit (Promega).
Li P., Lahvic J.L., Binder V., Pugach E.K., Riley E.B., Tamplin O.J., Panigrahy D., Bowman T.V., Barrett F.G., Heffner G.C., McKinney-Freeman S., Schlaeger T.M., Daley G.Q., Zeldin D.C, & Zon L.I. (2015). Epoxyeicosatrienoic Acids Enhance Embryonic Haematopoiesis and Adult Marrow Engraftment. Nature, 523(7561), 468-471.
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2

Zebrafish Embryo Proliferation and Apoptosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Zebrafish embryos were chemically treated between 48–72 hpf and fixed at 72 hpf. For proliferation analysis, embryos were permeablized and stained with primary antibody against phospho-Histone H3, and FITC-conjugated secondary antibody. Embryos were imaged and phospho-H3 positive cells in the CHT were manually counted. Secondary antibody-only control showed no non-specific staining. For apoptosis analysis, embryos were stained using the colorimetric TUNEL staining kit (Promega).
Li P., Lahvic J.L., Binder V., Pugach E.K., Riley E.B., Tamplin O.J., Panigrahy D., Bowman T.V., Barrett F.G., Heffner G.C., McKinney-Freeman S., Schlaeger T.M., Daley G.Q., Zeldin D.C, & Zon L.I. (2015). Epoxyeicosatrienoic Acids Enhance Embryonic Haematopoiesis and Adult Marrow Engraftment. Nature, 523(7561), 468-471.
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