Plasmocin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Plasmocin is a broad-spectrum antimicrobial agent used in cell culture to eliminate mycoplasma contamination. It targets and inhibits the growth of mycoplasma organisms in cell cultures.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Plasmocin prophylactic (5 citations)

36 protocols using plasmocin

Colon Cancer Cell-Myofibroblast Interaction Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colon cancer epithelial cells DLD-1 (Male, Dukes’s stage C), HCT116 (Male, Dukes’s stage D), and colonic myofibroblast CCD-18co cells (Female) were obtained from the Korean Cell Line Bank, and SW48 (Female, Dukes’s stage C) was purchased from ATCC. DLD-1 and HCT116 cells were cultured in RPMI-1640 (Gibco, Carlsbad, CA, USA) and CCD-18co and SW48 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, Carlsbad, CA, USA) with L-glutamine (300 mg/L) supplemented with 10% fetal bovine serum (Gibco), penicillin (100 U/mL), and streptomycin (100 μM). DLD-1 cells were treated with TGFβ (R&D systems, Minneapolis, MN, USA), PDGF-D (R&D systems), imatinib (Tocris Bioscience, Bristol, UK), 2-APB (Tocris), and ML-9 (Sigma Aldrich, St. Louis, MO, USA) for 8 or 16 h. The conditioned medium was obtained from the supernatant of cells collected 8 h after PDGF-D stimulation, and treated with fresh medium in a 1:1 ratio to CCD-18co cells. To prevent mycoplasma contamination, Plasmocin™ (Invitrogen, San Diego, CA, USA, ant-mpp) was added to the medium.

Kim M.S., Choi H.S., Wu M., Myung J., Kim E.J., Kim Y.S., Ro S., Ha S.E., Bartlett A., Wei L., Ryu H.S., Choi S.C., Park W.C., Kim K.Y, & Lee M.Y. (2020). Potential Role of PDGFRβ-Associated THBS4 in Colorectal Cancer Development. Cancers, 12(9), 2533.

+ Open protocol

+ Expand

Maintenance of Colorectal Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human colorectal adenocarcinoma HT-29, WE480, HCT 116, LoVo and SW620 cell lines and mouse colorectal adenocarcinoma MC38 cells were purchased from the National Collection of Authenticated Cell Cultures (https://cellbank.org.cn/, accessed on 20 August 2022), and authenticated by STR before the sale. Cells were maintained in a 37 °C incubator with 5% CO₂. The cell culture media were as follows: McCoy’s 5A (GIBCO, 16600082, Waltham, USA) with 10% FBS (Gibco, 10099-141, Waltham, USA) for HT-29 and HCT 116, L-15 (Hyclone, SH30525.01, Logan, USA) with 10% FBS (Gibco, 10099-141, Waltham, USA) for SW480 and SW620, Ham’s F-12K (Hyclone, SH30526.01) with 10% FBS (Gibco, 10099-141, Waltham, USA) for LoVo and DMEM (Hyclone, SH30243.01B) with 10% FBS (Gibco, 10099-141, Waltham, USA) for MC38, respectively. Penicillin-Streptomycin (Gibco, C14-15070-063, Waltham, USA) and plasmocin (Invitrogen, ant-mpp, Carlsbad, USA) were added to the cell culture media. All cells were checked for mycoplasma DNA every 3 months.

Kang B., Zhang X., Wang W., She S., Chen W., Chen C., Wang Y., Pan X., Xu O, & Wang Y. (2022). The Novel IGF-1R Inhibitor PB-020 Acts Synergistically with Anti-PD-1 and Mebendazole against Colorectal Cancer. Cancers, 14(23), 5747.

+ Open protocol

+ Expand

Culturing Murine and PDX Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adherent primary murine RT2-cancer cells (described above), B16-F10 melanoma cells (catalogue number CRL-6475, ATCC) and B16-OVA melanoma cells (gift from R. Dutton, Trudeau Institute, New York, USA) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% foetal calf serum (FCS), nonessential amino acids, sodium pyruvate, antibiotics, and 50 µM 2-mercaptoethanol at 37 °C and 7.5% CO₂. The murine melanoma cell lines B16 and B16-OVA were cultured in DMEM medium, containing 10% FCS and penicillin/streptomycin (100 U ml⁻¹; all from Biochrom AG), at 37 °C and 5% CO₂. The human patient-derived xenograft (PDX) cell lines were cultured in RPMI-1640 medium supplemented with 10% FCS, nonessential amino acids, sodium pyruvate, antibiotics, and 5 µg ml⁻¹ Plasmocin (Invitrogen) to treat infections of clinical samples, at 37 °C and 5.0% CO₂. Cell line supernatants were tested with a mycoplasma detection PCR Venor GeM (Minerva Biolabs GmbH) regularly every 4 weeks.

Brenner E., Schörg B.F., Ahmetlić F., Wieder T., Hilke F.J., Simon N., Schroeder C., Demidov G., Riedel T., Fehrenbacher B., Schaller M., Forschner A., Eigentler T., Niessner H., Sinnberg T., Böhm K.S., Hömberg N., Braumüller H., Dauch D., Zwirner S., Zender L., Sonanini D., Geishauser A., Bauer J., Eichner M., Jarick K.J., Beilhack A., Biskup S., Döcker D., Schadendorf D., Quintanilla-Martinez L., Pichler B.J., Kneilling M., Mocikat R, & Röcken M. (2020). Cancer immune control needs senescence induction by interferon-dependent cell cycle regulator pathways in tumours. Nature Communications, 11, 1335.

+ Open protocol

+ Expand

Cell Culture Protocols for Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

MV4-11, MOLM13, KOPN-8, RS4;11, CCRF-CEM, Jurkat, SET2, Jeko-1, Ramos, 293T, U251, SW620, and A673 cells were obtained from ATCC. Hut78 cells were received from Steven Rosen (City of Hope Cancer Center). MV4-11, MOLM13, KOPN-8, RS4;11, CCRF-CEM, Jurkat, SET2, Jeko-1, and Ramos cells were cultured in RPMI 1640 (Thermo Fisher Scientific) medium supplemented with 10% fetal bovine serum (FBS) (Omega Scientific). Hut78 was cultured in RPMI 1640 medium with 15% FBS. 293T, U251, SW620, and A673 cells were cultured in DMEM (Gibco) with 10% FBS. Penicillin/streptomycin (Gibco), GlutaMax (Gibco) and plasmocin (0.5 μg/ml; Invitrogen) were added to all media. All cells were cultured in 37°C incubator with 5% CO₂. Cells stably expressing the Cas9 endonuclease were established via transduction of LentiCas9-blast (Addgene) lentivirus and select by blasticidin (20 μg/ml; Gibco).

Liu Q., Chan A.K., Chang W.H., Yang L., Pokharel S.P., Miyashita K., Mattson N., Xu X., Li M., Lu W., Lin R.J., Wang S.Y, & Chen C.W. (2021). 3-Ketodihydrosphingosine reductase maintains ER homeostasis and unfolded protein response in leukemia. Leukemia, 36(1), 100-110.

+ Open protocol

+ Expand

Breast Cancer Cell Line Culture Conditions

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

MMTV-PyMT;Apc^+/+ and MMTV-PyMT;Apc^Min/+ cells were isolated as previously described [7 (link)] and grown in RPMI 1640 media with 10% fetal bovine serum (FBS), 1% penicillin/streptomycin (P/S), and 1:5000 plasmocin (Invitrogen, Waltham, MA, USA). HCC1954, HCC1937, HCC38, DU4475, HCC1187, HCC1569, HCC1395, HS578T, and ZR-75-1 cells were also maintained in RPMI 1640 media with 10% FBS, 1% P/S, and 1:5000 plasmocin. MCF-7, MDA-MB-231, and MDA-MB-468 cells were maintained in DMEM media supplemented with 10% FBS, 1% P/S, and 1:5000 plasmocin. BT20 and BT474 cells were maintained in DMEM/F-12 media with 10% FBS, 1% P/S, and 1:5000 plasmocin. SKBR3 cells were maintained in RPMI 1640 media supplemented with 10% FBS, 1% P/S, 1 mM sodium pyruvate, and 2 mM L-glutamine. All cells listed above were obtained from the American Type Culture Collection (ATCC) (Manassas, VA, USA). KTB21-hTERT, KTB37-hTERT, and KTB34-hTERT cells (acquired from the Komen Tissue Bank) were maintained in DMEM/F-12 media supplemented with 5% FBS, 1% P/S, 0.4 μg/mL hydrocortisone, 5.0 μg/mL insulin, 20 ng/mL EGF, and 24 mg/L adenine [32 (link)]. KTB cells were passaged only 10 times. All cells were routinely passaged and maintained at 37 °C with 5% CO₂.

Stefanski C.D., Arnason A., Maloney S., Kotsen J., Powers E., Zhang J.T, & Prosperi J.R. (2023). APC Loss Prevents Doxorubicin-Induced Cell Death by Increasing Drug Efflux and a Chemoresistant Cell Population in Breast Cancer. International Journal of Molecular Sciences, 24(8), 7621.

+ Open protocol

+ Expand

Culturing IRF3- and MAVS-deficient Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

IRF3-deficient and MAVS-deficient HEK293T cells were a kind gift from Veit Hornung and were cultured in DMEM (Sigma-Aldrich) supplemented with 10% FBS (Sigma-Aldrich), 100 U/ml penicillin (Sigma-Aldrich), 100 mg/ml streptomycin (Sigma-Aldrich) and 5 μg/ml plasmocin (Invitrogen). IRF3-deficient THP-1 cells were also a gift from Veit Hornung and were cultured in RPMI 1640 supplemented with 10% FBS (Sigma-Aldrich), 200 mM L-glutamine, 100 U/ml penicillin (Sigma-Aldrich), and 100 mg/ml streptomycin (Sigma-Aldrich). All cell lines were and maintained at 37°C with 5% CO₂. Knockout cells were generated as described in (19 (link)).

Dalskov L., Narita R., Andersen L.L., Jensen N., Assil S., Kristensen K.H., Mikkelsen J.G., Fujita T., Mogensen T.H., Paludan S.R, & Hartmann R. (2020). Characterization of distinct molecular interactions responsible for IRF3 and IRF7 phosphorylation and subsequent dimerization. Nucleic Acids Research, 48(20), 11421-11433.

+ Open protocol

+ Expand

Murine Breast Cancer Cell Transfection

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The murine breast cancer cell line 4 T1 (American Type Culture Collection, ATCC CRL-2539; Rockville, MD, USA) was transfected with a dsRed-expressing lentiviral vector (Discosoma sp. red fluorescent protein) [30 (link)]. Cells were cultured in RPMI-1640 medium (Bio-Whit-taker; Verviers, Belgium) containing fetal bovine serum (FBS;10%), Penicillin/Streptomycin (100 units/mL), non-essential amino acids (3.2%), plasmocin (0.005 mg/mL; InvitroGen; San Diego, CA, USA), and L-glutamine (400 mol/L; Lonza; Cologne, Germany), and were maintained in a humidified environment at 5% CO₂ and 37°C.

Yang N., Huang B., Tsinkalovsky O., Brekkå N., Zhu H., Leiss L., Enger P.Ø., Li X, & Wang J. (2014). A novel GFP nude rat model to investigate tumor-stroma interactions. Cancer Cell International, 14, 541.

+ Open protocol

+ Expand

Isolation and Culture of Primary Ear Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The primary fibroblasts of ear skin were obtained according to previously established methods (Zhang et al., 2018 (link)). Briefly, the skin samples were sterilized with 75% ethyl alcohol and washed with PBS, then cut into pieces and adhered to the culture dish after removing the hair and fat tissues. The fibroblasts can be outgrown in about one week. All cells were cultured in DMEM high glucose (Hyclone) medium containing 10% FBS (Gibco), 1% glutamax (Gibco), 1% penicillin/streptomycin (Gibco), 2.5 μg/mL plasmocin (Invitrogen) and 1 mol/L tenofovir under 37 °C, 5% CO₂ conditions.

Wang F., Zhang W., Yang Q., Kang Y., Fan Y., Wei J., Liu Z., Dai S., Li H., Li Z., Xu L., Chu C., Qu J., Si C., Ji W., Liu G.H., Long C, & Niu Y. (2020). Generation of a Hutchinson–Gilford progeria syndrome monkey model by base editing. Protein & Cell, 11(11), 809-824.

+ Open protocol

+ Expand

Fluorescent Protein-Labeled MCF-7 Cell Lines

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

MCF-7 from ATCC and grown in DMEM, supplemented with 10% fetal bovine serum (FBS), 1% l-glutamine, and 1% penicillin/streptomycin. MCF-7 Src-Bio-tk cell line was obtained by G418 selection (500 µg/ml) of MCF-7 transiently transfected with Src-Bio-tk-pcDNA3 and FACS sorted for CFP and YFP expression. MCF-7 MRLC-GFP was obtained by lentiviral infection of MCF-7 cells using lentiviral particles prepared with MRLC-GFP-pLL5.0 plasmid and protocols described in Priya et al. (2015) (link). MCF-7 E-cad-GFP was obtained through CRISPR genome editing as described in Liang et al. (2017) (link). All cell lines were maintained in low doses of Plasmocin (Invitrogen) and routinely tested for the presence of mycoplasma. For experiments, cells were seeded at 30–40% confluence 48 h before transfection with siRNA using RNAimax (Invitrogen, Cat #13778030) according to the manufacturer’s recommendations. For live-cell experiments, cells were cultured on 29-mm glass-bottom dishes (Shengyou Biotechnology) and imaged in clear Hank’s balanced salt solution supplemented with 5% FBS, 10 mM HEPES (pH 7.4), 5 mM CaCl₂, glucose 4,5 g/l, and ProLong Live Antifade Reagent for live-cell imaging (Thermo Fisher Scientific, Cat #P36975).

Teo J.L., Tomatis V.M., Coburn L., Lagendijk A.K., Schouwenaar I.M., Budnar S., Hall T.E., Verma S., McLachlan R.W., Hogan B.M., Parton R.G., Yap A.S, & Gomez G.A. (2020). Src kinases relax adherens junctions between the neighbors of apoptotic cells to permit apical extrusion. Molecular Biology of the Cell, 31(23), 2557-2569.

+ Open protocol

+ Expand

Skin Explant Culture to Assess CCG-1423 Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols

C57BL/6J mice were sacrificed at p26, backs shaved, skin removed, and then 6-mm punch (Integra; 33-36) was used to generate circular punches, which were subsequently attached to the bottom of 6-well TC plates (Corning; 3516) with 6uL of Matrigel (Corning; 356237). Explants were cultured in 154CF media (Gibco; M15CF500) supplemented with 15% chelated FBS (GE HyClone; SH30396.03), 1% Penicillin Streptomycin (Gibco; 15140-122), 0.05 mM CaCl2 (Gibco; 50-9702), 0.4% Amphotericin B (Gibco; 15290026), 0.01% Plasmocin (Invitrogen; NC9886956), and 1% HKGS (Gibco; S-0001-5). Paired explants from same mouse were either treated with 150 μm CCG-1423 (Tocris; 5277) or DMSO control. Explants were cultured for 72 h, then fixed in 4% PFA for 24 h and imbedded in paraffin blocks for H&E and immunofluorescence staining.

Yao C.D., Haensel D., Gaddam S., Patel T., Atwood S.X., Sarin K.Y., Whitson R.J., McKellar S., Shankar G., Aasi S., Rieger K, & Oro A.E. (2020). AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma. Nature Communications, 11, 5079.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!