Log In Sign up
The largest database of trusted experimental protocols

Kanamycin kan

Manufactured by Merck Group
Visit Supplier
Sourced in United States, France

Kanamycin (Kan) is an antibiotic that functions as a selection marker in molecular biology experiments. It inhibits bacterial protein synthesis, allowing for the identification and selection of cells that have successfully incorporated genetic constructs.

Automatically generated - may contain errors

Lab products found in correlation

Ampicillin amp, by Merck Group (9 mentions) Chloramphenicol cm, by Merck Group (6 mentions) Spectinomycin spc, by Merck Group (5 mentions) Cefoxitin cfx, by Merck Group (2 mentions) T4 dna ligase, by New England Biolabs (2 mentions) Chloramphenicol cm, by Thermo Fisher Scientific (2 mentions) Erythromycin erm, by Merck Group (2 mentions) Tetracycline tet, by Merck Group (2 mentions) Tryptic soy broth (tsb), by Thermo Fisher Scientific (1 mentions) Middlebrook 7h9 medium, by BD (1 mentions) Middlebrook 7h10 medium, by BD (1 mentions) Van91i, by Thermo Fisher Scientific (1 mentions) Q5 dna polymerase, by New England Biolabs (1 mentions) Hydrogen peroxide h2o2, by Merck Group (1 mentions) Ethambutol emb, by Merck Group (1 mentions) Levofloxacin lfx, by Merck Group (1 mentions) Cefepime, by Merck Group (1 mentions) Chloramphenicol, by Merck Group (1 mentions) Tetracycline, by Merck Group (1 mentions) P iodonitrotetrazolium chloride int, by Merck Group (1 mentions)

31 protocols using kanamycin kan

1

Cultivation and Antibiotic Conditions for Bacterial Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols
The bacterial strains and plasmids used in this study are listed in Table 1. R. anatipestifer strains were grown at 37°C in tryptic soybean broth (TSB, Oxoid) or tryptic soy agar (TSA, Oxoid) in an atmosphere of 5% CO2. Escherichia coli (E. coli) strains were grown on Luria-Bertani (LB, Oxoid) broth or agar at 37°C. When required, antibiotics were added at the following final concentrations (μg/ml): Chloramphenicol (Cm, Sigma), 25; cefoxitin (Cfx, Sigma), 1; kanamycin (Kan, Sigma), 100; ampicillin (Amp, Sigma), 100 or spectinomycin (Spc, Sigma), 70. Diaminopimelic acid (DAP, 50 μg/ml) to E. coli X7213λpir cultures (Edwards et al., 1998 (link)).
Huang L., Yuan H., Liu M.F., Zhao X.X., Wang M.S., Jia R.Y., Chen S., Sun K.F., Yang Q., Wu Y., Chen X.Y., Cheng A.C, & Zhu D.K. (2017). Type B Chloramphenicol Acetyltransferases Are Responsible for Chloramphenicol Resistance in Riemerella anatipestifer, China. Frontiers in Microbiology, 8, 297.
+ Open protocol
+ Expand
2

Bacterial Strain Interactions with Tetrahymena

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The bacterial strains and plasmids used in this study are listed in Table S1. A. hydrophila NJ-35 and its derivative non-Tetrahymena-exposed strain B1 (conventionally cultured without T. thermophila) and Tetrahymena-exposed strain SCV1 (a small colony variant (SCV) strain after cocultured with T. thermophila) were maintained in Luria-Bertani (LB) medium at 28°C, as previously described [12 (link)]. Escherichia coli SM10 was conventionally cultured in LB medium at 37°C [12 (link)]. When necessary, chloramphenicol (Cm) (Sigma Louis, MO, USA), kanamycin (Kan) (Sigma), or ampicillin (Amp) (Sigma) were added to the medium. T. thermophila SB210 (accession number GCA_000261185.1) was obtained from Dr. Miao Wei, Institute of Hydrobiology, China Academy of Sciences, and cultured in SPP medium (2% protease peptone, 0.1% yeast extract, 0.2% glucose, 0.003% EDTA-Fe) at 28°C [12 (link)]. The lytic bacteriophage G65, belonging to the family Myoviridae, was isolated from a contaminated river in Nanjing, China, in 2014 [20 (link)].
Dong Y., Liu J., Nie M., Zhao D., Huang H., Geng J., Wan X., Lu C, & Liu Y. (2022). Comparative transcriptome combined with morphophysiological analyses revealed the molecular mechanism underlying Tetrahymena thermophila predation-induced antiphage defense in Aeromonas hydrophila. Virulence, 13(1), 1650-1665.
+ Open protocol
+ Expand
3

Characterization of A. hydrophila NJ-35 Strain

Check if the same lab product or an alternative is used in the 5 most similar protocols
The bacterial strains and plasmids that were used in this study are listed in Table 3. A. hydrophila NJ-35, which belongs to the ST251 clonal group, was isolated from diseased cultured crucian carp in the Jiangsu province of China in 2010 [40 (link)]. The genome sequence of A. hydrophila NJ-35 has been published in GenBank (accession number CP006870). A. hydrophila and E. coli were cultured in Luria Bertani broth (LB) at 28 and 37 °C, respectively. When necessary, chloramphenicol (Cm) (Sigma Louis, MO, USA), kanamycin (Kan) (Sigma), or ampicillin (Amp) (Sigma) were added to the medium.
Dong Y., Wang Y., Liu J., Ma S., Awan F., Lu C, & Liu Y. (2018). Discovery of lahS as a Global Regulator of Environmental Adaptation and Virulence in Aeromonas hydrophila. International Journal of Molecular Sciences, 19(9), 2709.
+ Open protocol
+ Expand
4

Mycobacterium smegmatis Growth Media and Reagents

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
As previously described13 (link), the 7H9 liquid culture medium for M. smegmatis strains consisted of Middlebrook 7H9 medium (Becton Dickinson, Franklin Lakes, New Jersey, U.S.) supplemented with 10% ADS (5% (w/v) bovine serum albumin fraction V, 2% (w/v) dextrose and 8.1% (w/v) NaCl), 0.5% (v/v) glycerol, and 0.05% (v/v) Tween80. 7H10 media containing Middlebrook 7H10 medium (Becton Dickinson, Franklin Lakes, New Jersey, U.S.), 10% ADS, and 0.5% (v/v) glycerol was used for solid culture to examine growth status. Hygromycin (Hyg) was purchased from GenView, erythromycin (EM) from Merck, hydrogen peroxide (H2O2) and kanamycin (Kan) from Sigma. Restriction enzymes such as Van91I, AlwNI, MfeI, and PacI were purchased from Fermentas. T4 DNA ligase and Q5 DNA polymerase were purchased from New England Biolabs.
Li X., Li J., Hu X., Huang L., Xiao J., Chan J, & Mi K. (2015). Differential roles of the hemerythrin-like proteins of Mycobacterium smegmatis in hydrogen peroxide and erythromycin susceptibility. Scientific Reports, 5, 16130.
+ Open protocol
+ Expand
5

Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis

Check if the same lab product or an alternative is used in the 5 most similar protocols
All experiments using live clinical Mtb strains were conducted in the biosafety level 2 plus laboratory of Wuhan Pulmonary Hospital. All strains were tested for drug susceptibility against 6 anti-TB drugs according to the World Health Organization (WHO) and Clinical and Laboratory Quality Institute (CLSI) guidelines. Löwenstein-Jensen commercial media (Wuhan, China) containing antibiotics were used in the proportion methods to test all antibiotics. The critical concentrations for INH (Sigma, Oakville, ON, Canada) were 0.2 mg/L, RIF (Sigma) 40.0 mg/L, ethambutol (EMB, Sigma) 2.0 mg/L, levofloxacin (LFX, Sigma) 2.0 mg/L, moxifloxacin (MOX, Sigma) 2.0 mg/L, and kanamycin (KAN, Sigma) 30.0 mg/L, respectively. After 3 weeks of incubation at 37 °C, their DST results were confirmed (Table S1).
Ullah N., Hao L., Banga Ndzouboukou J.L., Chen S., Wu Y., Li L., Borham Mohamed E., Hu Y, & Fan X. (2021). Label-Free Comparative Proteomics of Differentially Expressed Mycobacterium tuberculosis Protein in Rifampicin-Related Drug-Resistant Strains. Pathogens, 10(5), 607.
+ Open protocol
+ Expand
6

Isolation and Identification of Phytochemicals from Nauclea pobeguinii

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Compounds previously isolated from the bark of Nauclea pobeguinii included 3-acetoxy-11-oxo-urs-12-ene (1), p-coumaric acid (2), citric acid trimethyl ester (3), resveratrol (4), resveratrol β-D-glucopyranoside (5) and strictosamide (6) (Fig. 1). Their isolation and identification were previously reported [25 (link)]. Tetracycline (TET), cefepime (CEP), ciprofloxacin (CIP), chloramphenicol (CHL), ampicillin (AMP), streptomycin (STR), kanamycin (KAN) (Sigma-Aldrich, St Quentin Fallavier, France) were used as reference antibiotics (RA). p-Iodonitrotetrazolium chloride (INT; Sigma-Aldrich) and Phenylalanine-Arginine-ß-Naphthylamide (PAßN; Sigma-Aldrich) were used as microbial growth indicator and efflux pumps inhibitor (EPI) respectively [27 (link), 28 (link)].

Chemical structures of the compounds isolated from Nauclea pobeguinii.1: 3-acetoxy-11-oxo-urs-12-ene; 2:p-coumaric acid; 3: citric acid trimethyl ester; 4: resveratrol; 5: resveratrol β-D-glucopyranoside; 6: strictosamide

Seukep J.A., Sandjo L.P., Ngadjui B.T, & Kuete V. (2016). Antibacterial and antibiotic-resistance modifying activity of the extracts and compounds from Nauclea pobeguinii against Gram-negative multi-drug resistant phenotypes. BMC Complementary and Alternative Medicine, 16, 193.
+ Open protocol
+ Expand
7

Antibiotic Potentiation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nine commonly used antibiotics including tetracycline (TET), cefepime (CEP), streptomycin (STR), ciprofloxacin (CIP), norfloxacin (NOR), chloramphenicol (CHL), ampicillin (AMP), erythromycin (ERY), kanamycin (KAN) (Sigma-Aldrich, St Quentin Fallavier, France) were used for potentiation assay. p-Iodonitrotetrazolium chloride 0.2% (INT) and phenylalanine arginine β-naphthylamide (PAβN) (Sigma-Aldrich) were used as bacterial growth indicator and efflux pumps inhibitor respectively. Dimethylsulfoxide 10% (DMSO) was used as solvent for all extracts.
Touani F.K., Seukep A.J., Djeussi D.E., Fankam A.G., Noumedem J.A, & Kuete V. (2014). Antibiotic-potentiation activities of four Cameroonian dietary plants against multidrug-resistant Gram-negative bacteria expressing efflux pumps. BMC Complementary and Alternative Medicine, 14, 258.
+ Open protocol
+ Expand
8

Quantifying Bacterial Internalization Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The internalization assay was performed as previously described [26 (link)]. In brief, after 2 h of bacterial adhesion, the culture medium containing extracellular bacteria was removed. The infected cells were then washed 3 times with PBS, pH 7.5. Medium containing 250 μg/ml kanamycin (KAN) (Sigma-Aldrich) was added and further incubated for 2 h to inhibit the growth of residual extracellular bacteria. After 4 h of incubation, the cells were washed and lysed as described above. The numbers of internalized bacteria in the lysate were quantified using the drop plate technique on LB agar. The percentage of bacteria that had internalized was calculated from three independent experiments, with triplicate determinations each time.
Mattrasongkram P., Wongkaewkhiaw S., Taweechaisupapong S., Chareonsudjai S., Techawiwattanaboon T., Ngamsiri T, & Kanthawong S. (2023). Vitamin D (1α,25(OH)2D3) supplementation minimized multinucleated giant cells formation and inflammatory response during Burkholderia pseudomallei infection in human lung epithelial cells. PLOS ONE, 18(2), e0280944.
+ Open protocol
+ Expand
9

Anaerobic Growth Conditions for Bacterial Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Strains and plasmids used in this study are listed in Table 1. All strains were grown in overnight cultures from single-use aliquots of cryopreserved cells, diluted 1000-fold in BHI media (Beckinson Dickinson). Mutant strains were incubated with the appropriate antibiotics: kanamycin (Kan; Sigma-Aldrich) 500 μg mL−1; erythromycin (Erm; Sigma-Aldrich); 10 μg mL−1; spectinomycin (Spc; Sigma-Aldrich) 200 μg mL−1; chloramphenicol (Cm; Fisher) 5 μg mL−1. The pre-cultures of the SK36 ΔssaACB ΔtmpA and VMC66 ΔssaACB ΔmntH and ΔssaACB ΔmntH ΔtmpA required exogenous Mn2+ for growth (15 μM for the triple mutant; 10 μM for the others). The cultures were then incubated at 37°C for 16–20 h (or up to 24 h for the triple mutant) with the atmospheric condition set to 1% O2 (9.5% H2, 9.5% CO2, and 80% N2) or 6% O2 (7% H2, 7% CO2, and 80% N2) using a programmable Anoxomat™ Mark II jar-filling system (AIG, Inc).
Puccio T., An S., Schultz A.C., Lizarraga C.A., Bryant A.S., Culp D.J., Burne R.A, & Kitten T. (2021). Manganese transport by Streptococcus sanguinis in acidic conditions and its impact on growth in vitro and in vivo. Molecular Microbiology, 117(2), 375-393.
+ Open protocol
+ Expand
10

Cultivation and Genetic Manipulation of Mycobacterium tuberculosis and Escherichia coli

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mtb H37Ra (purchased from ATCC) was cultivated in Middlebrook 7H9 medium containing 0.05% glycerol and 10% oleic acid–albumin–dextrose–catalase (OADC) enrichment. Escherichia coli BL21 (DE3) was incubated in Luria-Bertani (LB) medium and used for protein expression. The restriction enzymes, T4 DNA Ligase, Taq DNA polymerase, and DNase I were purchased from New England Biolabs (Beverly, MA, USA) and Thermo Fisher Scientific (Waltham, MA, USA). All antibiotics, including kanamycin (Kan) and ampicillin (Amp), were purchased from Sigma-Aldrich (Darmstadt, Germany). Antiserum was obtained from Dia-An Biotechnology Co., Ltd., (Wuhan, China). All constructed plasmids and strains are listed in Table S1.
Wei W., Jiang X., Zhang L., Yan Y., Yan J., Xu L., Gao C.H, & Yang M. (2023). Regulation of CRISPR-Associated Genes by Rv1776c (CasR) in Mycobacterium tuberculosis. Biomolecules, 13(2), 400.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!