Anti yap1 primary antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The Anti-YAP1 primary antibody is a laboratory reagent used in research applications. It specifically detects the YAP1 protein, which is a transcriptional regulator involved in the Hippo signaling pathway. This antibody can be used to identify and study the expression and localization of YAP1 in various cell and tissue samples.

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Lab products found in correlation

Normal rabbit igg antibody, by Cell Signaling Technology (1 mentions) Chip assay kit, by Beyotime (1 mentions) Vcx 750, by Sonics (1 mentions)

Close spelling variants of this product

Anti-YAP1 (47 citations) YAP1 antibody (7 citations) Anti-YAP1 antibody (6 citations)

2 protocols using anti yap1 primary antibody

ChIP Assay of YAP1-TEAD Binding

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ChIP assays were performed according to the manufacturer's instructions using a ChIP Assay Kit (Beyotime, cat. no. 2078). Using Flag-YAP1 and TEADs plasmids transfection, ChIP was performed in BGC-823 cells. The cells were lysed by ultrasonication (SONICS VCX750, USA) and the supernatant was treated with magnetic beads coupled with either a normal rabbit IgG antibody or an anti-YAP1 primary antibody (Cell Signaling Technology, cat. no. D8H1X) in an immunoprecipitation buffer overnight at 4 °C. Beads were then cleaned using a washing buffer, and the immunoprecipitated RNA was subjected to RT-qPCR test for analysis. The experiments were conducted according to a previous study [23 (link)]. The primer sequences used for the ChIP assay are listed in Supplementary Table S5.

Li X., Zhong H., Shi Q., Ruan R., Huang C., Wen Q., Zeng S., Xia Y., Zeng Q., Xiong J., Wang S., Chen J., Lei W, & Deng J. (2024). YAP1-CPNE3 positive feedback pathway promotes gastric cancer cell progression. Cellular and Molecular Life Sciences: CMLS, 81(1), 143.

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Immunohistochemical Evaluation of YAP1 in MTSCC

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In the current study, IHC were performed on representative whole tumor sections from the MTSCC cases with anti-YAP1 primary antibody (Cell Signaling Technology: / Danvers, MA / 01923 YAP1 Rabbit polyclonal cat# 4912s; 1 in 300 dilution). Formalin fixed, paraffin sections were cut at 5 microns and rehydrated to water. Heat induced epitope retrieval was performed with FLEX TRS Low pH Retrieval buffer (6.1) for 20 minutes (Dako, North America: / Carpentaria, CA / 93013; FLEX TRS Low pH Retrieval Buffer, FLEX HRP EnVision Detection System). After peroxidase blocking, the antibody YAP1 rabbit polyclonal was applied at a dilution of 1:300 at room temperature for 60 minutes. The FLEX HRP EnVision System was used for detection. DAB chromagen was then applied for 10 minutes. Slides were counterstained with Harris Hematoxylin for 5 seconds, dehydrated and coverslipped. IHC was assessed for nuclear and cytoplasmic expression on tumor cells and background benign renal parenchyma by two study pathologists (A.U. and R.M.).

Mehra R., Vats P., Cieslik M., Cao X., Su F., Shukla S., Udager A.M., Wang R., Pan J., Kasaian K., Lonigro R., Siddiqui J., Premkumar K., Palapattu G., Weizer A., Hafez K.S., Wolf JS J.r., Sangoi A., Trpkov K., Osunkoya A.O., Zhou M., Giannico G., McKenney J.K., Dhanasekaran S.M, & Chinnaiyan A.M. (2016). Bi-allelic Alteration and Dysregulation of the Hippo Pathway in Mucinous Tubular and Spindle Cell Carcinoma of the Kidney. Cancer discovery, 6(11), 1258-1266.

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