Spss statistic version 24

The statistical analysis used IBM SPSS STATISTIC (Version 24). An analysis of variance (ANOVA) and Tukey's HSD multiple comparison tests (p < .05) was carried out to establish the difference between pairs of groups, according to the physicochemical and sensory parameters.

We performed an analysis by dividing all children into three categories according to age: <1 year, 1–2 years, and >2 years. The duration of symptoms was analyzed by dividing it into early and late groups, <6 h and ≥6 h, respectively. Based on initial assessment by the initial attending physician, the children were assigned to either the diagnosed or undiagnosed group. The undiagnosed group included patients who were discharged from the ER, admitted on the suspicion of other diseases, and patients who were diagnosed with intussusception based on the US or CT scanning after suspecting other diseases (for example, appendicitis) in the ER.
Continuous variables are presented as medians with ranges or means ± standard deviations. An independent Student's t-test or one-way analysis of variance was applied for continuous variables. Categorical variables were reported using frequencies and proportions and were compared using the Pearson χ² or Fisher's exact test, as appropriate, or by linear association. The data were analyzed by using the Statistical Program for the Social Sciences (IBM SPSS Statistic Version 24, IBM Inc., Chicago, IL). A P-value of <0.05 was considered to be statistically significant.

The statistical analysis was done using probit analysis from IBM SPSS Statistic Version 24 to find the knockdown time of 50% and 95% of the tested population (KdT50 and KdT95). Based on the KdT50 and KdT95 values, the resistance ratio (RR) was calculated as in the formula below: $$RR=\frac{KdT50/95ofthefieldstrain}{KdT50/95ofthesusceptiblestrain}$$
Based on the RR value, the field population is considered as susceptible when the RR < 5, mosquitoes are considered to have a moderate resistance when RR is between 5 and 10, and the mosquitoes are highly resistant when RR > 10 ²⁶ .
In contrast, the percentage of mortality of the tested population was recorded after 24 h by using the formula as follows: $$Observedmortality=\frac{Totalnumberofdeadmosquitoes}{Totalsamplesize}\times 100$$
Abbott`s formula is used as the corrective formula if the mortality rate of control replicates ranged between 10 and 20%. However, the test was discarded and repeated if the percentage in control mortality was more than ≥ 20%. Abbott’s formula was not applied in this study. $$Correctedmortality=\frac{\%Observedmortality-\%Controlmortality}{100-\%Controlmortality}\times 100$$
The sequences obtained from the sequencing company (My TAGC DNA Sequencing Services) were aligned using ClustalW and translated into a protein sequence by using protein translation tool from Mega v7.0 software^{29 (link)}.

The variables were collected just before and after starting mechanical ventilation. Each lamb served as his or her own control. All statistical analyses were conducted using SPSS Statistic version 24 (IBM corporation, Armonk, NY, USA). Continuous variables were mean ± standard deviation after checking for normal distribution of the data using the Shapiro–Wilk test. The non-parametric Friedman and Wilcoxon distribution-free test were used to assess the significance of differences in respiratory and hemodynamic measures between measurements.