Dithiothreitol (DTT), isopropyl 1-thio-β-D-galactopyranoside (IPTG), Triton X-100, α-ketoglutaric acid sodium salt (αKG), DL-isocitric acid trisodium salt hydrate, and magnesium chloride (MgCl2) were obtained from Fisher Scientific (Hampton, NH). BME was obtained from MP Biomedicals (Santa Ana, CA). β-Nicotinamide adenine dinucleotide phosphate reduced trisodium salt (NADPH), β-Nicotinamide adenine dinucleotide phosphate disodium salt (NADP+) and tris(2-carboxyethyl)phosphine) (TCEP) was purchased from Millipore Sigma (Burlington, MA). Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA). Stain free gels (4–12%) were obtained from Bio-Rad Laboratories (Hercules, CA). Protease inhibitor tablets were obtained from Roche Applied Science (Penzberg, Germany). Phenylmethylsulfonyl fluoride (PMSF) salt was purchased from Thermo Scientific (Waltham, MA). The Escherichia coli BL21 Gold DE3 strain was used for all protein expression.
Phenylmethylsulfonyl fluoride pmsf salt
Manufactured by Thermo Fisher Scientific
Phenylmethylsulfonyl fluoride (PMSF) is a chemical compound commonly used as a protease inhibitor in biological research and laboratory applications. It functions by irreversibly inhibiting the enzymatic activity of serine proteases, which are a class of enzymes involved in various cellular processes. PMSF is widely used to preserve the integrity of protein samples during extraction, purification, and analysis procedures.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Thermo Fisher Scientific (3 mentions)
Protease inhibitor tablet, by Roche (3 mentions)
Dithiothreitol (dtt), by Thermo Fisher Scientific (3 mentions)
Isopropyl 1 thio β d galactopyranoside, by Thermo Fisher Scientific (3 mentions)
Nickel nitrilotriacetic acid ni nta resin, by Qiagen (3 mentions)
α ketoglutaric acid sodium salt, by Thermo Fisher Scientific (3 mentions)
Dl isocitric acid trisodium salt hydrate, by Thermo Fisher Scientific (3 mentions)
β nicotinamide adenine dinucleotide phosphate reduced trisodium salt, by Merck Group (3 mentions)
β nicotinamide adenine dinucleotide phosphate disodium salt nadp and tris 2 carboxyethyl phosphine tcep, by Merck Group (3 mentions)
Stain free gel, by Bio-Rad (2 mentions)
3 protocols using phenylmethylsulfonyl fluoride pmsf salt
1
Bacterial Expression and Purification
2
Recombinant Protein Expression and Purification
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Dithiothreitol (DTT), isopropyl 1-thio-β-d -galactopyranoside (IPTG), Triton X-100, α-ketoglutaric acid sodium salt (αKG), dl -isocitric acid trisodium salt hydrate, and magnesium chloride (MgCl2) were obtained from Fisher Scientific (Hampton, NH). BME was obtained from MP Biomedicals (Santa Ana, CA). β-Nicotinamide adenine dinucleotide phosphate reduced trisodium salt (NADPH), β-Nicotinamide adenine dinucleotide phosphate disodium salt (NADP+) and tris(2-carboxyethyl)phosphine) (TCEP) was purchased from Millipore Sigma (Burlington, MA). Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA). Stain free gels (4–12%) were obtained from Bio-Rad Laboratories (Hercules, CA). Protease inhibitor tablets were obtained from Roche Applied Science (Penzberg, Germany). Phenylmethylsulfonyl fluoride (PMSF) salt was purchased from Thermo Scientific (Waltham, MA). The Escherichia coli BL21 Gold DE3 strain was used for all protein expression.
3
Purification of Recombinant Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dithiothreitol (DTT), isopropyl 1-thio-β-D-galactopyranoside (IPTG), Triton X-100, α-ketoglutaric acid sodium salt (αKG), DL-isocitric acid trisodium salt hydrate, and magnesium chloride (MgCl2) were obtained from Fisher Scientific (Hampton, NH). BME was obtained from MP Biomedicals (Santa Ana, CA). β-Nicotinamide adenine dinucleotide phosphate reduced trisodium salt (NADPH), β-nicotinamide adenine dinucleotide phosphate disodium salt (NADP+) and tris(2-carboxyethyl)phosphine) (TCEP) was purchased from Millipore Sigma (Burlington, MA). Nickel-nitrilotriacetic acid (Ni-NTA) resin was obtained from Qiagen (Valencia, CA). Stain free gels (4–12%) were obtained from Bio-Rad Laboratories (Hercules, CA). Protease inhibitor tablets were obtained from Roche Applied Science (Penzberg, Germany). Phenylmethylsulfonyl fluoride (PMSF) salt was purchased from Thermo Scientific (Waltham, MA).
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