G6410b triple quadrupole mass spectrometer

Manufactured by Agilent Technologies

Sourced in United States

The G6410B triple quadrupole mass spectrometer is a laboratory instrument designed for quantitative and qualitative analysis of chemical compounds. It utilizes triple quadrupole technology to perform precise mass analysis and detection of target analytes in complex samples.

Automatically generated - may contain errors

Lab products found in correlation

Calf intestinal phosphatase, by New England Biolabs (2 mentions) Nuclease p1, by Merck Group (2 mentions) Agilent 1290 uhplc system, by Agilent Technologies (2 mentions) C18 column, by Agilent Technologies (1 mentions) 1290 uplc, by Agilent Technologies (1 mentions) Zorbax sb c18, by Agilent Technologies (1 mentions) Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions)

Spelling variants of this product

Spectrometers (10 citations)

5 protocols using g6410b triple quadrupole mass spectrometer

Quantification of m6A RNA Modifications

Check if the same lab product or an alternative is used in the 5 most similar protocols

In brief, 100–200 ng of mRNA was digested by 0.1 U Nuclease P1 (Sigma) and 1.0 U Calf Intestinal Phosphatase (New England Biolabs) and incubated at 37°C overnight. The sample was then filtered (MW cutoff: 3 kDa, Pall, Port Washington), and subjected to LC-MS/MS. The nucleosides were separated by reversed-phase ultra-performance liquid chromatography on an Agilent C18 column with online mass spectrometry detection using a G6410B triple quadrupole mass spectrometer (Agilent Technologies) in the positive ion mode. The m⁶A levels were calculated as the ratio of m⁶A to A based on the calibrated concentrations according to the standard curve obtained from pure nucleoside standards running with the same batch of samples.

Shi Y., Dou Y., Zhang J., Qi J., Xin Z., Zhang M., Xiao Y, & Ci W. (2021). The RNA N6-Methyladenosine Methyltransferase METTL3 Promotes the Progression of Kidney Cancer via N6-Methyladenosine-Dependent Translational Enhancement of ABCD1. Frontiers in Cell and Developmental Biology, 9, 737498.

+ Open protocol

+ Expand

UHPLC-MS/MS Analysis of Modified Mononucleosides

Check if the same lab product or an alternative is used in the 5 most similar protocols

UHPLC-MS/MS analysis of modified mononucleosides was carried out according to Yin et al. (34 (link)). It was performed on an Agilent 1290 UHPLC system coupled with a G6410B triple quadrupole mass spectrometer (Agilent Technologies, Palo Alto, CA, USA). An isocratic elution with 5.0% methanol, 95% water and 0.1% formic acid running at 0.25 ml/min was used for UHPLC separation of mononucleosides. The eluate from the column was injected into electrospray ionization-triple quadrupole mass spectrometry. Positive multiple reaction monitoring modes were used: m/z 242→126 for 5mC (collision energy, 5eV); m/z 258→142 for 5hmC (5eV); m/z 256→140 for 5fC (5eV); m/z 272→156 for 5caC (5eV). 2′-deoxyguanosine (dG) in all samples was also measured to normalize the amount of DNA on column. Each sample was analyzed at least three times. The frequency of modified mononucleosides was calculated by corresponding standard curves.

Li Z., Gu T.P., Weber A.R., Shen J.Z., Li B.Z., Xie Z.G., Yin R., Guo F., Liu X., Tang F., Wang H., Schär P, & Xu G.L. (2015). Gadd45a promotes DNA demethylation through TDG. Nucleic Acids Research, 43(8), 3986-3997.

+ Open protocol

+ Expand

Quantification of Coenzyme Q10 and Q9

Check if the same lab product or an alternative is used in the 5 most similar protocols

An Agilent G6410B triple-quadrupole mass spectrometer (equipped with `Hotbox' upgrade) was coupled to an Agilent 1290 UPLC with binary pump, autosampler and column compartment. The LC column was an Agilent Zorbax SB-C18, 30 mm × 2.1 mm × 3.5 μm maintained at 40°C. The mobile phase was methanol containing 5 mM ammonium formate pumped at a rate of 0.8 mL/min. The run time was 4.2 min per injection.
The mass spectrometer source was operated in the positive ion electrospray mode. Drying gas was 10 L/min of nitrogen at 350°C. Nebulizer gas was nitrogen at 35 psig. Capillary voltage was 4000 V. The mass spectrometer was operated in the MRM mode with the following transitions. Coenzyme Q10 (oxidized): m/z 863.7→197.1, fragmentor = 200 V, CE = 41 V, dwell = 200 ms. Coenzyme Q10 (reduced): m/z 882.7→197.1, fragmentor = 160 V, CE = 25 V, dwell = 200 ms. Coenzyme Q9 (oxidized form): 795.6→197.1, fragmentor = 190 V, CE = 33 V, dwell = 200 ms. Coenzyme Q9 (reduced form): 814.7→197.1, fragmentor = 150 V, CE = 25 V, dwell = 200 ms.
Calibration curves were generated with peak heights and analysed with a second order polynomial fit by the MassHunter WorkStation.

Claessens A.J., Yeung C.K., Risler L.J., Phillips B.R., Himmelfarb J, & Shen D.D. (2015). Rapid and sensitive analysis of reduced and oxidized coenzyme Q10 in human plasma by ultra performance liquid chromatography-tandem mass spectrometry and application to studies in healthy human subjects. Annals of clinical biochemistry, 53(Pt 2), 265-273.

+ Open protocol

+ Expand

LC-MS/MS Analysis of m6A Methylation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC–MS/MS analysis was conducted as previously reported (49–51 (link)). In brief, 100–200 ng of mRNA was digested by 0.1 U Nuclease P1 (Sigma) and 1.0 U calf intestinal phosphatase (New England Biolabs) in a final reaction volume of 50 μl, adjusted with water and incubated at 37°C overnight. The sample was then filtered (MW cutoff: 3 kDa, Pall, Port Washington) and subjected to LC–MS/MS analysis for detection of m⁶A. The nucleosides were separated by reverse phase ultra-performance liquid chromatography on a C18 column with online mass spectrometry detection using a G6410B triple quadrupole mass spectrometer (Agilent Technologies) in the positive ion mode. The nucleosides were quantified by using the nucleoside-to-base ion mass transitions of m/z 282 to 150 (m⁶A) and m/z 268 to 136 (A). Quantification was carried out by comparison with a standard curve obtained from pure nucleoside standards run with the same batch of samples. The ratio of m⁶A to A was calculated based on the calibrated concentrations.

Wu Z., Shi Y., Lu M., Song M., Yu Z., Wang J., Wang S., Ren J., Yang Y.G., Liu G.H., Zhang W., Ci W, & Qu J. (2020). METTL3 counteracts premature aging via m6A-dependent stabilization of MIS12 mRNA. Nucleic Acids Research, 48(19), 11083-11096.

+ Open protocol

+ Expand

UHPLC-MS/MS Analysis of Mononucleosides

Check if the same lab product or an alternative is used in the 5 most similar protocols

The DNA products were digested into mononucleosides and subjected to ultra-performance liquid chromatograph-tandem mass spectrometry (UHPLC-MS/MS) analysis as described previously (14 (link),39 ). Briefly, the UHPLC-MS/MS analysis was performed on Agilent 1290 UHPLC system coupled with a G6410B triple quadrupole mass spectrometer with an electrospray ionization source (Agilent Technologies, Santa Clara, CA, USA). A reversed-phase Zorbax Eclipse Plus C18 column (100 mm × 2.1 mm i.d., 1.8-μm particle size, Agilent Technologies) was used for separation. A MassHunter workstation software version B.01.03 was used for data acquisition. The mobile phase consisted of 5.0% methanol and 95% water (plus 0.1% formic Acid) and was used for UHPLC separation of the mononucleosides at a flow rate of 0.3 ml/min.

Zhao C., Wang H., Zhao B., Li C., Yin R., Song M., Liu B., Liu Z, & Jiang G. (2014). Boronic acid-mediated polymerase chain reaction for gene- and fragment-specific detection of 5-hydroxymethylcytosine. Nucleic Acids Research, 42(9), e81.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!