The cells were stained with a PI/RNase mixture from Beyotime Biotechnology (Shanghai, China), particularly in the dark, at 37 °C for 30 min to the cell cycle analysis. Then, the stained cells were tested in a FACSCalibur system (Beckman Coulter, Brea, CA, USA).
Pi rnase mixture
Manufactured by Beyotime
Sourced in China
The PI/RNase mixture is a solution that contains a combination of protease inhibitor (PI) and RNase inhibitor. The PI prevents the degradation of proteins by proteases, while the RNase inhibitor prevents the degradation of RNA by RNase enzymes. This mixture is commonly used in molecular biology and biochemistry applications to preserve the integrity of proteins and RNA during sample preparation and analysis.
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Lab products found in correlation
2 protocols using pi rnase mixture
1
Cell Cycle Analysis by PI/RNase Staining
2
Cell Cycle Analysis of Transfected Cells
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According to the instructions of the Cell Cycle Analysis Kit (Beyotime), cells were cultured in 6‐well plates and were transfected with miR‐NC, miR‐133b, si‐NC, or si‐FOXL2 for 72 h. The cells were then collected, washed with cold PBS, fixed in 70% ethanol at 4°C for 24 h, and washed with cold PBS again. The cells were stained with a PI/RNase mixture from Beyotime Biotechnology, particularly in the dark, at 37°C for 30 min for the cell cycle analysis. Then, the stained cells were tested in a fluorescence‐activated cell sorting Caliber system (Beckman Coulter).
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