Ultra rtx 5ms

The chemical constituents of oleoresins were analyzed by GC-MS (Shimadzu QP 2010 plus instrument) with GCMS-QP 2010 Ultra DB-5 and GCMS-QP 2010 Ultra Rtx-5MS columns (30 m × 0.25 mm i.d., 0.25 µm). The experimental conditions are as follows: Carrier gas: helium with flow rate = 1.21 mL/min, and split ratio = 10.0. Oven temperature: 50–280°C with a temperature gradient of 3°C/min upto 210°C (isotherm for 2 min), and then 6°C/min upto 280°C. Identification of oleoresins components was performed by comparing their relative retention index (RI) values with mass spectra NIST (NIST version 2.1) and WILEY (7th edition) libraries, and also by matching the fragmentation pattern of the mass spectral data with those reported in the literature [21 ].

To check the chemical diversity in tested Vitex species, the essential oils were analyzed by GC-MS (Shimadzu QP 2010 plus) with GCMS-QP 2010 Ultra DB-5 and GCMS-QP 2010 Ultra Rtx-5MS column (30 m × 0.25 mm i.d., 0.25 µm). The following experimental conditions used helium as the carrier gas (flow rate = 1.21 mL/min, split ratio = 10.0). Oven temperature was programmed at 50–280 °C with a temperature gradient of 3 °C/min up to 210 °C (isotherm for 2 min), then 6 °C/min up to 280 °C. Identification of essential oil components was done by comparing their relative retention index (RI) values with mass spectra NIST (NIST version 2.1) and WILEY (7th edition) libraries, and by matching the fragmentation pattern of the mass spectral data with those reported in the literature [25 ,26 (link)].