MPO inhibition effects of the peptides were measured by using an MPO inhibitor screening assay kit (Abcam PLC, Cambridge, UK) following the instruction in the enclosed user manuals. Briefly, 10 µL of each peptide, 10 µL of 1.25 µL/mL MPO and 40 µl of assay buffer were mixed in a 96-well black plate. 50 µL of the peroxidation initiator solution was quickly added to all of the wells and then incubated for 5 min at RT. After incubation, the fluorescence intensity of the each well was read using an excitation wavelength of 530 nm and an emission wavelength of 590 nm.
Mpo inhibitor screening assay kit
Manufactured by Abcam
Sourced in United Kingdom, United States
The MPO inhibitor screening assay kit is a laboratory tool designed to measure the activity of myeloperoxidase (MPO) and screen for potential MPO inhibitors. The kit provides a standardized protocol and necessary reagents to perform the assay.
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Lab products found in correlation
2 protocols using mpo inhibitor screening assay kit
1
Peptide MPO Inhibition Assay
2
MPO Inhibitor Screening Assay Protocol
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The definition of the batch conditions was based on an MPO inhibitor screening assay kit from Abcam® (Waltham, MA, USA) [39 ].
For each assay, we used a mixture of 25 μL of 0.40 U/mL of MPO, 2.5 μL to 3% of H2O2, 125 μL of 200 μM of ADHP and 97.5 μL of PBS buffer pH of 7.4, creating a final volume of 250 μL. The reaction was measured after 5 min at 37 °C. The excitation and emission wavelengths were 530 and 590 nm, respectively. Each assay was carried out in duplicate. The formula used to calculate inhibition by this method was the same as used for SIA methodology.
For each assay, we used a mixture of 25 μL of 0.40 U/mL of MPO, 2.5 μL to 3% of H2O2, 125 μL of 200 μM of ADHP and 97.5 μL of PBS buffer pH of 7.4, creating a final volume of 250 μL. The reaction was measured after 5 min at 37 °C. The excitation and emission wavelengths were 530 and 590 nm, respectively. Each assay was carried out in duplicate. The formula used to calculate inhibition by this method was the same as used for SIA methodology.
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