The analytical standard of saccharin (>99.5%) was obtained from Aladdin (Shanghai, China). Methanol (≥99.8%), ethyl acetate (≥99.5%), acetic acid (≥99.5%), l -ascorbic acid (≥99.8%), sodium hydroxide (≥99.5%), sodium borohydride (≥96%), silver nitrate (≥99.8%), glass fiber membrane, aluminum foil and beakers were obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). Class backed silica gel F254 plates (20 × 10 cm, analytical grade, serial No. 4022536067940) were supplied by Merck (Darmstadt, Germany). Prior to using, each plate was pre-washed with Methanol, followed by drying at 120 °C for 20 min on a TLC Plate Heater (CAMAG, Muttenz, Switzerland). Four beverage samples (1-iced tea, 2-cola, 3-salt soda water, 4-alcoholic drink) were purchased at local supermarkets.
Class backed silica gel f254 plates
Manufactured by Merck Group
Sourced in Germany
Class backed silica gel F254 plates are thin-layer chromatography (TLC) plates used for the separation and identification of chemical compounds. The plates are coated with a layer of silica gel, which serves as the stationary phase. The F254 designation indicates the presence of a fluorescent indicator that allows for the visualization of separated compounds under ultraviolet (UV) light.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Tlc plate heater, by CAMAG (1 mentions)
Ethyl acetate, by Sinopharm (1 mentions)
Acetic acid, by Sinopharm (1 mentions)
Methanol, by Sinopharm (1 mentions)
Sodium borohydride, by Sinopharm (1 mentions)
Silver nitrate, by Sinopharm (1 mentions)
L ascorbic acid, by Sinopharm (1 mentions)
Synergy system, by Merck Group (1 mentions)
2 protocols using class backed silica gel f254 plates
1
Determination of Saccharin in Beverages
2
Quantifying Tyramine in Cheese Samples
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Tyramine hydrochloride (>98% purity) and fluorescamine (>99% purity) as well as methanol, ethyl acetate were purchased from Aladdin (Shanghai, China). Ultra-pure water was prepared by a Synergy system (Millipore, Schwalbach, Deutschland). Other chemicals and solvents of analytical grade were from Sigma Aldrich (Shanghai, China). Class backed silica gel F254 plates (20 × 10 cm, analytical grade, serial No. 4022536067940) was supplied by Merck (Darmstadt, Deutschland). Prior using, each plate was pre-washed with methanol. All cheese samples were purchased at local supermarkets.
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