L isoleucine 13c6 15n

BBR, γ-oryzanol, pyridoxine (vitamin B₆), urease, pyridine, methoxylamine hydrochloride, L-phenylalanine-¹³C₉-¹⁵N, dulcitol, L-leucine-¹³C₆, L-isoleucine-¹³C₆-¹⁵N, L-valine-¹³C₅-¹⁵N, L-alanine-¹³C₃-¹⁵N and N,O-bis(trimethylsilyl)-trifluoroacetamide (BSTFA) with 1 % trimethylchlorosflane (TMCS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pure water was obtained from Millipore Alpha-Q water system (Bedford, MA, USA). Methanol and ethanol for HPLC grade were purchased from Merck Chemicals (Darmstadt, Germany). Chloroform for analytical grade from Sinopharm Chemical Reagent Company (Shanghai, China). KAPA HiFi polymerase (KAPA Biosystems, USA), Agencourt AMPure XP beads (Beckman Coulter, USA), Qubit dsDNA HS assay kit (Invitrogen, CA), Agilent high sensitivity DNA Kit (Agilent, USA), Ion PGM template OT2 200 kit, Ion PGM sequencing 200 kit v2 and Ion 316 chip kit v2 (Life Technology, CA) and QIAamp^® Fast DNA stool mini kit (Qiagen, Germany) were obtain for fecal microbiota analysis.

BBR hydrochloride (purity ≥99%) was purchased from Shanghai Ronghe Pharmaceutical Technology Development Company (Shanghai, China). L-leucine-¹³C₆-¹⁵N, L-isoleucine-¹³C₆-¹⁵N, L-valine-¹³C₅-¹⁵N, L-alanine-¹³C₃-¹⁵N, methoxylamine hydrochloride, dulcitol and N,O-bis(trimethylsilyl)-trifluoroacetamide (BSTFA) with 1% trimethylchlorosilane (TMCS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Methanol, ethanol and isopropanol for HPLC grade were purchased from Merck Chemicals (Darmstadt, Germany). Chloroform of analytical grade was purchased from Sinopharm Chemical Reagent Company (Shanghai, China). Trizol reagent was purchased from Invitrogen (Carlsbad, CA, USA).