Mops morpholinepropanesulfonic acid ph 7
Manufactured by Merck Group
MOPS (morpholinepropanesulfonic acid [pH 7]) is a laboratory buffer solution used to maintain a stable pH environment in various biological and chemical applications. It is a zwitterionic organic compound that acts as a good buffer in the physiological pH range. This product is commonly used in cell culture, enzyme assays, and other biochemical experiments where a consistent pH is crucial for optimal performance.
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Lab products found in correlation
Mgcl2, by Thermo Fisher Scientific (2 mentions)
Glycerol, by Thermo Fisher Scientific (2 mentions)
Cacl2, by Thermo Fisher Scientific (2 mentions)
Fecl3, by Merck Group (2 mentions)
Zncl2, by Merck Group (2 mentions)
Potassium phosphate, by Thermo Fisher Scientific (2 mentions)
Glutamate, by Merck Group (2 mentions)
Mncl2, by Thermo Fisher Scientific (2 mentions)
Thiamine hydrochloride, by Thermo Fisher Scientific (2 mentions)
B subtilis biofilm promoting medium, by Thermo Fisher Scientific (2 mentions)
2 protocols using mops morpholinepropanesulfonic acid ph 7
1
Bacillus subtilis Biofilm Cultivation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
Bacillus subtilis NCIB 3610 amyE::PtapA-Ypet strain (ES2107) was used for all experiments. The strain was cultured on lysogeny broth (LB)-agar plates prepared from Miller LB (Acros Organics, Fair Lawn, NJ) and 1.5% agar powder (Molecular Genetics, Fisher Scientific, Waltham, MA). LB agar plates were streaked with B. subtilis ES2107 from frozen stocks and incubated at 30°C for 15 h.
B. subtilis biofilms were grown on MSgg, a B. subtilis biofilm-promoting medium (5 mM potassium phosphate [pH 7]) (Fisher Scientific, Waltham, MA), 100 mM MOPS (morpholinepropanesulfonic acid [pH 7]) (Sigma-Aldrich, St. Louis, MO), 2 mM MgCl2 (Fisher Scientific, Waltham, MA), 700 μM CaCl2 (Alfa Aesar, Haverhill, MA), 50 μM MnCl2 (Fisher Scientific, Waltham, MA), 50 μM FeCl3 (Sigma-Aldrich, St. Louis, MO), 1 μM ZnCl2 (Sigma-Aldrich, St. Louis, MO), 2 μM thiamine hydrochloride (Fisher Scientific, Waltham, MA), 0.5% glycerol (Fisher Scientific, Waltham, MA), and 0.5% glutamate (Sigma-Aldrich, St. Louis, MO). For MSgg agar plates, 1.5% agar (BD) was added to the medium, and approximately 5-ml plates were poured.
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2
Bacillus subtilis Biofilm Cultivation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
Bacillus subtilis NCIB 3610 amyE::PtapA-Ypet strain (ES2107) was used for all experiments. The strain was cultured on lysogeny broth (LB)-agar plates prepared from Miller LB (Acros Organics, Fair Lawn, NJ) and 1.5% agar powder (Molecular Genetics, Fisher Scientific, Waltham, MA). LB agar plates were streaked with B. subtilis ES2107 from frozen stocks and incubated at 30°C for 15 h.
B. subtilis biofilms were grown on MSgg, a B. subtilis biofilm-promoting medium (5 mM potassium phosphate [pH 7]) (Fisher Scientific, Waltham, MA), 100 mM MOPS (morpholinepropanesulfonic acid [pH 7]) (Sigma-Aldrich, St. Louis, MO), 2 mM MgCl2 (Fisher Scientific, Waltham, MA), 700 μM CaCl2 (Alfa Aesar, Haverhill, MA), 50 μM MnCl2 (Fisher Scientific, Waltham, MA), 50 μM FeCl3 (Sigma-Aldrich, St. Louis, MO), 1 μM ZnCl2 (Sigma-Aldrich, St. Louis, MO), 2 μM thiamine hydrochloride (Fisher Scientific, Waltham, MA), 0.5% glycerol (Fisher Scientific, Waltham, MA), and 0.5% glutamate (Sigma-Aldrich, St. Louis, MO). For MSgg agar plates, 1.5% agar (BD) was added to the medium, and approximately 5-ml plates were poured.
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