Phospho fak py397

Transforming growth factor-β1 (TGF-β1) and platelet derived growth factor BB (PDGF-BB) were obtained from R&D Systems (Minneapolis, MN). The following antibodies were purchased: phospho-FAK [pY397] (Biosource, Camarillo, CA), procollagen alpha 1 type 1 (1A1), Fyn, Cyclin D1 (Santa Cruz Biotechnology, Santa Cruz, CA), FAK (N-terminal domain) (Santa Cruz, CA), Fyn and phospho-SrcY418 (Cell Signaling Technology, Boston, MA), phosphor-ERK and ERK, phosphor-p38 and p38, Cleaved caspase-3, cleaved Poly-(ADP-ribose) polymerase (PARP), (EMD Millipore, Billerica, MA), and glyceraldehyde 3-phosphate dehydrogenase (G3PDH) (Research Diagnostics, Flanders, NJ). Anti-α-SMA antibody, Cy3-conjugated Anti-α-SMA antibody, carbon tetrachloride (CCl₄), corn oil, and OptiPrep were purchased from Sigma-Aldrich (St. Louis, MO). FAK inhibitor (PF-562271) was purchased from Selleckchem and Fisher Scientific (Waltham, MA). Kits for active Rac and Rho were purchased from EMD Millipore (Billerica, MA). The generation and production of adenoviral vectors containing FAK-related non-kinase (ad-FRNK) or control green fluorescent protein (GFP) were described previously^{26 (link)}. All other chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO) and Fisher Scientific (Waltham, MA).