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Leibovitz s l 15 cell culture media

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Leibovitz's L-15 cell culture media is a formulation designed to support the growth and maintenance of various cell types in vitro. It is a complete, serum-supplemented medium that provides the necessary nutrients and growth factors for cell proliferation and survival. The core function of this media is to create an optimal environment for culturing cells outside of their natural biological context.

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Lab products found in correlation

Xcite 120q fluorescence lamp, by Excelitas (1 mentions) Ix81 inverted epifluorescence microscope, by Olympus (1 mentions)

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Cell culture media (342 citations) Leibovitz’s L-15 (97 citations) Culture media (67 citations) L-15 media (65 citations) Leibovitz’s L-15 media (38 citations) Leibovitz L-15 media (33 citations) Leibovitz L-15 (24 citations) Leibovitz-15 media (4 citations) Leibovitz's 15 media (2 citations) Leibovitz’s media (2 citations)

3 protocols using leibovitz s l 15 cell culture media

1

High-Resolution Live-Cell Imaging of Actin Cytoskeleton

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Fluorescent live-cell and fixed-cell images were obtained using an Olympus IX81 inverted epifluorescence microscope equipped with an Olympus UPLSAPO 60xW/1.2 NA objective, and a box & temperature & CO2 controller (Life Imaging Sciences). Live-cell imaging was performed 3-4 hrs after seeding the cells on the micropillar substrates. An imaging setup was developed to enable use of the high NA objective with micropillar substrates for high quality live-cell imaging of actin cytoskeleton. Briefly, the micropillar substrate with live cells was mounted on a glass coverslip, which was then inverted over another coverslip with spacers of thin (≈ 300 μm) silicone membranes (Specialty Manufacturing Inc., USA), preventing the cells from getting squeezed. The coverslips were mounted in a holder (Bioptechs Inc., USA) for imaging. Live-cell images were taken every 10 s or 2 min for 30 min or 2 hrs respectively. Leibovitz’s L-15 cell culture media (Life Technologies), supplemented with 10% FBS was used to minimize background.
Gupta M., Sarangi B.R., Deschamps J., Nematbakhsh Y., Callan-Jones A., Margadant F., Mège R.M., Lim C.T., Voituriez R, & Ladoux B. (2015). Adaptive rheology and ordering of cell cytoskeleton govern matrix rigidity sensing. Nature communications, 6, 7525.
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2

High-Resolution Live-Cell Imaging of Actin Cytoskeleton

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fluorescent live-cell and fixed-cell images were obtained using an Olympus IX81 inverted epifluorescence microscope equipped with an Olympus UPLSAPO 60xW/1.2 NA objective, and a box & temperature & CO2 controller (Life Imaging Sciences). Live-cell imaging was performed 3-4 hrs after seeding the cells on the micropillar substrates. An imaging setup was developed to enable use of the high NA objective with micropillar substrates for high quality live-cell imaging of actin cytoskeleton. Briefly, the micropillar substrate with live cells was mounted on a glass coverslip, which was then inverted over another coverslip with spacers of thin (≈ 300 μm) silicone membranes (Specialty Manufacturing Inc., USA), preventing the cells from getting squeezed. The coverslips were mounted in a holder (Bioptechs Inc., USA) for imaging. Live-cell images were taken every 10 s or 2 min for 30 min or 2 hrs respectively. Leibovitz’s L-15 cell culture media (Life Technologies), supplemented with 10% FBS was used to minimize background.
Gupta M., Sarangi B.R., Deschamps J., Nematbakhsh Y., Callan-Jones A., Margadant F., Mège R.M., Lim C.T., Voituriez R, & Ladoux B. (2015). Adaptive rheology and ordering of cell cytoskeleton govern matrix rigidity sensing. Nature communications, 6, 7525.
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3

Live-cell Imaging Microscopy Protocol

Cited 1 time
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Fluorescent live-cell and fixed-cell images were obtained using an Olympus IX81 inverted epifluorescence microscope equipped with an Olympus UPLSAPO 60x W/1.2 NA objective, a CoolSNAP EZ CCD camera (Photometrics), an X-Cite ® 120Q fluorescence lamp (Excelitas Technologies), and temperature and CO2 controller (Life Imaging Sciences). Leibovitz’s L-15 cell culture media (Life Technologies) supplemented with 10% fetal bovine serum and 1% penicilin/streptomycin was used for live-cell imaging to minimize background. Live-cell imaging was done in an upside-down configuration as described previously [13 (link), 19 (link)]. Live cells were imaged 3-7 hours after seeding.
Gupta M., Doss B.L., Kocgozlu L., Pan M., Mège R.M., Callan-Jones A., Voituriez R, & Ladoux B. (2019). Cell shape and substrate stiffness drive actin-based cell polarity. Physical review. E, 99(1-1), 012412.
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