Lightcyler faststart dna master plus sybr green 1 kit

RNA was isolated using the Direct-zol™ RNA Mini Prep Kit (Zymo Research, Freiburg) and complementary DNA was synthesized using SuperScript Reverse Transcriptase (Life Technologies). qRT-PCR was performed with a Light Cycler 2.0 using a LightCyler FastStart DNA Master PLUS SYBR Green I Kit (Roche Diagnostics). The qRT-PCR results were normalized using mouse aryl hydrocarbon receptor-interacting protein (AIP) as house keeping gene. The following primers (Thermo Fisher Scientific) were used: AIP (forward: 5′-GCTCCGTTATAGATGACAGC-3′, reverse: 5′-ATCTCGATGTGGAAGATGAG-3′), arginase 1 (forward: 5′-TCCTTTCAAATTGTGAAGAACCCACGGTC-3′, reverse: 5′-AGAATCCTGGTACATCTGGGAACTTTCCT-3′), iNOS (forward: 5′-AGCAATGGGCAGACTCTGAAGAAATCTC-3′, reverse: 5′-ATGTTTGCTTCGGACATCAAAGGTCTCAC-3′).

RNA was isolated and complementary DNA was synthesized as previously described^{19 (link)} and qRT-PCR was performed with a CFX Connect™ Real-Time PCR Detection System (Bio-Rad) using a LightCyler FastStart DNA Master PLUS SYBR Green I Kit (Roche Diagnostics). The qRT-PCR results were normalized using mouse aryl hydrocarbon receptor-interacting protein (AIP) as house keeping gene. Primer sets (Thermo Fisher Scientific) used are listed in Supplementary Table 2.