Ferric chloride

Manufactured by HiMedia

Sourced in India

Ferric chloride is a chemical compound with the formula FeCl3. It is a crystalline solid that is hygroscopic and readily dissolves in water. Ferric chloride is commonly used in water treatment, as a coagulant, and in various industrial processes.

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Lab products found in correlation

Sodium hydroxide (naoh), by HiMedia (7 mentions) Hydrogen peroxide, by HiMedia (6 mentions) Potassium ferricyanide, by HiMedia (6 mentions) Ascorbic acid, by HiMedia (6 mentions) Trichloroacetic acid, by HiMedia (6 mentions) Sodium nitrite, by HiMedia (5 mentions) Potassium persulfate, by HiMedia (5 mentions) Sodium carbonate, by HiMedia (5 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by HiMedia (5 mentions) Gallic acid, by HiMedia (5 mentions) Gallic acid, by Merck Group (4 mentions) Nitro blue tetrazolium, by HiMedia (4 mentions) Methanol, by HiMedia (4 mentions) Hydrochloric acid (hcl), by HiMedia (3 mentions) Sulfuric acid, by HiMedia (3 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Eosin yellow, by HiMedia (3 mentions) Mercury 2 oxide red, by Merck Group (3 mentions) Ethanol, by HiMedia (3 mentions) Phenazine methosulfate, by HiMedia (3 mentions)

Spelling variants of this product

Ferric chloride hexahydrate (3 citations) Ferric chloride (FeCl3), (2 citations) Ferric chloride hexahydrate A.R (2 citations)

28 protocols using ferric chloride

Extraction and Characterization of Melanin

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Standard melanin (synthetic) and 5,5′-dithio-bis (2-nitrobenzoic acid) were purchased from Sigma-Aldrich, USA. Keratin powder, bovine serum albumin, sodium hydroxide, hydrogen peroxide, ferric chloride, sodium hypochlorite, and potassium hydroxide were obtained from Himedia, India. Solvents, such as ethanol, methanol, chloroform, acetone, benzene, and phenol were procured from Sisco Research Laboratory (SRL), India. All the chemicals and reagents used were of the highest purity and analytical grade. Black feathers of slaughtered domestic fowl (Gallusgallus domesticus) collected from central chicken market, Kolhapur, India were washed, dried, and stored for further studies. Whereas, goat (Capra hircus-Osmanabadi breed) skin was obtained from the local meat market in Kolhapur, India.

Gurav R.G., Tang J, & Jadhav J.P. (2016). Sulfitolytic and keratinolytic potential of Chryseobacterium sp. RBT revealed hydrolysis of melanin containing feathers. 3 Biotech, 6(2), 145.

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Bioactive Compound Screening from Microbes

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All reagents used for the experiment were analytical grade purchased from Himedia (HiMedia Laboratories Pvt. Ltd., Mumbai, India) including silver nitrate (Mw: 169.87 g/mol; and purity: ≥99.9%), hydrogen peroxide, iodine, potassium iodide (Mw 166.00 g/mol and purity: 99%), sodium hydroxide (M: 39.997 g/mol and purity: 97%), sulfuric acid, chloroform, benzene, ammonia, naphthol, ethanol, copper acetate, ferric chloride (Mw: 162.2 g/mol and purity: ≥99%), Butylated hydroxytoluene (BHT), hydrochloric acid, potato dextrose agar, nutrient agar, potassium ferricyanide, TCA, α-amylase, etc. Bacterial culture including Bacillus subtilis (MTCC 121T), Shigella flexneri (MTCC 1457), Bacillus megaterium (NCTC 6094), Trichoderma viride, Aspergillus niger (MTCC 12975), and Penicillium crysogenum (NCPF 2802) were collected from Dayanand Science College, Latur, India.

Nagime P.V., Singh S., Shaikh N.M., Gomare K.S., Chitme H., Abdel-Wahab B.A., Alqahtany Y.S., Khateeb M.M., Habeeb M.S, & Bakir M.B. (2023). Biogenic Fabrication of Silver Nanoparticles Using Calotropis procera Flower Extract with Enhanced Biomimetics Attributes. Materials, 16(11), 4058.

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Ashwagandha and Autoimmune Arthritis

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For the study, Ashwashila (Batch no: AH18/038, manufacturing date: April 2018) was sourced from the Divya Yoga Pharmacy, Haridwar, India, 5-Clone Cocktail antibodies (Cat No-53040) and LPS (Escherichia coli strain 0111: B4; Cat No-9028) were purchased from Chondrex, Inc. WA. Methotrexate (Cat No-M9929) was procured from Sigma Aldrich, St. Louis, MO. Haematoxylin, Potassium Aluminium Sulphate Dodecahydrate, Mercury (II) Oxide red were purchased from Merck India Pvt Ltd, Mumbai, India. Safranin and Fast green were procured from Loba Chemie Pvt. Ltd, Mumbai, India. Eosin Yellow and Ferric Chloride were purchased from Hi-Media Laboratories, Mumbai, India. For tissue culture work, RPMI-1640 cell culture media, Fetal bovine serum, antibiotics, and other reagents were purchased from Life Technologies, Delhi, India.

Balkrishna A., Sakat S.S., Joshi K., Paudel S., Joshi D., Joshi K., Ranjan R., Gupta A., Bhattacharya K, & Varshney A. (2019). Herbo-mineral formulation ‘Ashwashila’ attenuates rheumatoid arthritis symptoms in collagen-antibody-induced arthritis (CAIA) mice model. Scientific Reports, 9, 8025.

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Antibacterial Zinc-Citrate Nanoparticles

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Chemicals used in this study include ciprofloxacin hydrochloride (MP Biomedicals, Santa Ana, CA, USA), zinc nitrate hexahydrate (98%, Sigma Aldrich, USA), trisodium citrate dihydrate (Sigma Aldrich, St. Louis, MO, USA), ferric chloride (Himedia, Mumbai, India), Luria agar and Luria broth (Himedia, Mumbai, India), sodium hydroxide (Merck, Kenilworth, NJ, USA), and hydrochloric acid (35.5%, Merck). All the chemicals were of molecular grade.

Das S., Ghosh S., Misra A.J., Tamhankar A.J., Mishra A., Lundborg C.S, & Tripathy S.K. (2018). Sunlight Assisted Photocatalytic Degradation of Ciprofloxacin in Water Using Fe Doped ZnO Nanoparticles for Potential Public Health Applications. International Journal of Environmental Research and Public Health, 15(11), 2440.

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Comprehensive Phytochemical Analysis Procedure

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Ethanol, mEthanol, chloroform, petroleum ether, ethyl acetate, diethyl ether, acetone, n-hexane, gallic acid standard, rutin standard, ascorbic acid, Folin–Ciocalteu’s phenol reagent, sodium carbonate, sodium nitrite, sodium hydroxide, aluminium chloride, ferric chloride, and potassium persulfate were obtained from Himedia Laboratories, Mumbai, India. 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tripyridyl-s-triazine (TPTZ), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma Aldrich, Bengaluru, India. All other analytical grade chemicals and reagents were obtained from Himedia Laboratories, Mumbai, India. Distilled water was used for all experiments.

Kunjiappan S., Ramasamy L.K., Kannan S., Pavadai P., Theivendren P, & Palanisamy P. (2024). Optimization of ultrasound-aided extraction of bioactive ingredients from Vitis vinifera seeds using RSM and ANFIS modeling with machine learning algorithm. Scientific Reports, 14, 1219.

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Phytochemical Analysis of Medicinal Plants

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Acetic anhydride, ammonium hydroxide, AA, butylated hydroxytoluene, 2-deoxy-2-ribose, DPPH, ferric chloride, gallic acid, magnesium ribbon, quercetin, sodium nitrite, and trichloroacetic acid were obtained from HiMedia Laboratories, Pvt., Ltd., Mumbai, India. Thiobarbituric acid was obtained from Loba Chemical, Mumbai, India. Ammonium persulphate, benzene, chloroform, di-potassium hydrogen phosphate, ethanol, ethylenediaminetetraacetic acid, glacial acetic acid, hydrogen peroxide, methanol, 5% o-phosphoric acid, 0.01% naphthylethylene diamine, potassium di-hydrogen phosphate, potassium ferricyanide, potassium hydroxide, sodium hydroxide, and sulfanilamide were obtained from Rankem, Mumbai, India. ABTS and gallic acid (3,4,5-trihydroxy benzoic acid) were obtained from Sigma, USA. Conc. HCl, conc. H₂ SO₄, Fehling's solution, Mayer's reagents, and sodium carbonate were procured from Merck, Mumbai, India. Folin-Ciocalteu reagent was from Sisco Research Laboratory, Mumbai, India. Aluminium chloride was obtained from SD Fine Chemicals Limited, Mumbai, India. All chemicals and solvents were of analytical grade.

Middha S.K., Goyal A.K., Lokesh P., Yardi V., Mojamdar L., Keni D.S., Babu D, & Usha T. (2015). Toxicological Evaluation of Emblica officinalis Fruit Extract and its Anti-inflammatory and Free Radical Scavenging Properties. Pharmacognosy Magazine, 11(Suppl 3), S427-S433.

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Antioxidant and Reducing Assays Protocol

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Mueller-Hinton broth, gallic acid, 2, 2-diphenyl-1- picrylhydrazyl (DPPH) and nitroblue tetrazolium chloride (NBT) were purchased from Sigma. The Folin Ciocalteu's reagent (FCR), phenazine methosulfate (PMS), nicotinamide adenine dinucleotide, reduced (NADH), potassium ferrocyanide, trichloroacetic acid (TCA), ferric chloride and hydrogen peroxide were procured from Himedia. All the solvents and chemical not mentioned were of analytical grade.

Upadhyay A., Chattopadhyay P., Goyary D., Mazumder P.M, & Veer V. (2014). Euphorbia hirta accelerates fibroblast proliferation and Smad-mediated collagen production in rat excision wound. Pharmacognosy Magazine, 10(Suppl 3), S534-S542.

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Antioxidant and Cytotoxicity Assay Protocol

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2.4.6-Tripyridyl-s-Triazine (TPTZ), 1,1-diphenyl, 2-picrylhydrazyl radical (DPPH), 2,2-Azinobis (3-ethyl benzothiazoline-6-sulfonic acid) (ABTS), Eagle’s minimum essential medium, trypsin (0.1 %), MTT [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide], fetal calf serum, DCFH-DA (2′,7′-dichlorofluorescein-diacetate), HS-RT- PCR kit, and RNA- isolation kit were purchased from Sigma, USA. Cyber green master mix (2X) was purchased from Qiagen (Gamb, Germany). Ascorbic acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), ferric chloride, hydrogen peroxide, and potassium persulfate were purchased from Himedia, Mumbai, India. Lactate dehydrogenase kit was purchased from Agappe diagnostics, Kerela, India.

Ghaffari H., Ghassam B.J., Chandra Nayaka S., Ramachandra Kini K, & Prakash H.S. (2014). Antioxidant and Neuroprotective Activities of Hyptis suaveolens (L.) Poit. Against Oxidative Stress-Induced Neurotoxicity. Cellular and Molecular Neurobiology, 34(3), 323-331.

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Antioxidant Capacity Evaluation Protocol

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1,1-Diphenyl,2-picryl hydrazyl (DPPH), nitroblue tetrazolium (NBT), riboflavin, L-methionine, thiobarbituric acid (TBA), ethylenediaminetetraacetic acid (EDTA), ascorbic acid, gallic acid, rutin and trichloroacetic acid (TCA), potassium ferricyanide (“K₃[Fe(CN)₆]”), and ferric chloride (“FeCl₃”) were purchased from Hi-Media Ltd. All reagents were of analytical grade.

Upadhyay R., Chaurasia J.K., Tiwari K.N, & Singh K. (2014). Antioxidant Property of Aerial Parts and Root of Phyllanthus fraternus Webster, an Important Medicinal Plant. The Scientific World Journal, 2014, 692392.

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Phytochemical and Antioxidant Analysis

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Ascorbic acid, 2,2-diphenyl-1-picryl-hydrazyl (DPPH), ferric chloride, gallic acid, naphthyl ethylenediamine dihydrochloride, quercetin, sodium nitrite (NaNO₂), sulphanilamide, and trichloroacetic acid were obtained from HiMedia Laboratories Pvt. Ltd., Mumbai, India. Di-potassium hydrogen phosphate, hydrogen peroxide, methanol, potassium di-hydrogen phosphate, potassium ferricyanide, potassium hydroxide, sodium hydroxide (NaOH), sodium carbonate (Na₂CO₃), sodium acetate, and sodium nitroprusside were procured from Merck, Mumbai, India, and Folin–Ciocalteu (FC) reagent was purchased from Sisco Research Laboratories Pvt. Ltd., Mumbai, India. Aluminum chloride (AlCl₃) and ortho-phosphoric acid were obtained from SD Fine Chemicals Limited, Mumbai, India. All chemicals and solvents were of analytical grade.

Usha T., Pradhan S., Goyal A.K., Dhivya S., Kumar H.P., Singh M.K., Joshi N., Basistha B.C., Murthy K.R., Selvaraj S, & Middha S.K. (2017). Molecular Simulation-based Combinatorial Modeling and Antioxidant Activities of Zingiberaceae Family Rhizomes. Pharmacognosy Magazine, 13(Suppl 3), S715-S722.

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