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Gentra blood kit

Manufactured by Qiagen
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The Gentra Blood Kit is a laboratory equipment product designed for the extraction and purification of genomic DNA from whole blood samples. It provides a simple and efficient method for obtaining high-quality DNA suitable for downstream applications such as PCR, sequencing, and genotyping.

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Lab products found in correlation

Novaseq 6000, by Illumina (1 mentions) Qubit fluorometer, by Qiagen (1 mentions) Hiseq 4000, by Illumina (1 mentions) Biomek fx robot, by Beckman Coulter (1 mentions) Accel ngs 2s dna library kit, by Integrated DNA Technologies (1 mentions)

Close spelling variants of this product

Gentra Puregene Blood Kit (569 citations) Gentra Purgene Blood Kit (9 citations) Gentra Puregene Blood and Tissue Extraction Kit (4 citations) Blood kits (3 citations) Gentra Puregene Blood Core Kit B (3 citations) Gentra Puragene Blood Kit (3 citations) Gentra Puregene blood extraction kit (3 citations) Gentra Puregene Blood Core Kit (3 citations) Gentra Puregene Blood Kit Plus (2 citations) Gentra Puregene Blood Core Kit C (2 citations)

6 protocols using gentra blood kit

1

Genomic DNA Extraction from Blood

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Genomic DNA from peripheral blood leukocytes was isolated using Gentra Blood Kit (Qiagen Corp, CA).
Acar S., BinEssa H.A., Demir K., Al-Rijjal R.A., Zou M., Çatli G., Anık A., Al-Enezi A.F., Özışık S., Al-Faham M.S., Abacı A., Dündar B., Kattan W.E., Alsagob M., Kavukçu S., Tamimi H.E., Meyer B.F., Böber E, & Shi Y. (2018). Clinical and genetic characteristics of 15 families with hereditary hypophosphatemia: Novel Mutations in PHEX and SLC34A3. PLoS ONE, 13(3), e0193388.
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2

Genomic DNA Extraction from Blood

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Genomic DNA was isolated from peripheral blood lymphocytes using the Gentra Blood Kit (Qiagen Corp., CA).
Zou M., Guven A., BinEssa H.A., Al-Rijjal R.A., Meyer B.F., Alzahrani A.S, & Shi Y. (2020). Molecular Analysis of CYP27B1 Mutations in Vitamin D-Dependent Rickets Type 1A: c.590G > A (p.G197D) Missense Mutation Causes a RNA Splicing Error. Frontiers in Genetics, 11, 607517.
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3

Genomic DNA Extraction and Sequencing

Cited 1 time
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The GENTRA Blood kit (Qiagen N.V.) was used for the isolation of genomic DNA from EDTA blood samples. The quantification and quality of the obtained DNA were assessed using the Qubit fluorometer (Qiagen N.V.). DNA was paired-end sequenced (read length of 150 base pair) as single-indexed genomic libraries using the Illumina Novaseq6000 (Illumina Inc., USA). Finally, raw reads were preprocessed by trimming the adapter sequences and removing the reads with 50% of low-quality nucleotides and fewer than 36 base pairs in length with fastp v0.23.1 [26 (link)].
Gonzalez-Prendes R., Ginja C., Kantanen J., Ghanem N., Kugonza D.R., Makgahlela M.L., Groenen M.A, & Crooijmans R.P. (2022). Integrative QTL mapping and selection signatures in Groningen White Headed cattle inferred from whole-genome sequences. PLOS ONE, 17(10), e0276309.
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4

Genomic DNA Isolation from Leukocytes

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Genomic DNA from peripheral blood leukocytes of patients was isolated using the Gentra Blood Kit (Qiagen Corp., CA) after informed consent was obtained from their parents. The parents and three unaffected siblings (two brothers and one sister) of the patients refuse to give blood samples for genetic test, which prevented us from performing a familial genetic analysis. The study was approved by our institutional review board.
Alqahtani M.A., Shati A.A., Zou M., Alsuheel A.M., Alhayani A.A., Al-Qahtani S.M., Gilban H.M., Meyer B.F, & Shi Y. (2015). A Novel Mutation in the CYP11B1 Gene Causes Steroid 11β-Hydroxylase Deficient Congenital Adrenal Hyperplasia with Reversible Cardiomyopathy. International Journal of Endocrinology, 2015, 595164.
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5

Exome Sequencing of the ClinSeq A2 Cohort

Cited 1 time
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Exome data generated from the ClinSeq® A2 cohort was used as the control dataset for this study [19 (link)]. All 502 individuals in this cohort were free from apparent somatic overgrowth disorders at the time of enrollment. Genomic DNA was isolated from peripheral blood using standard protocols (Gentra Blood Kit; Qiagen, Gaithersburg, MD). Sequencing was performed at the NIH Intramural Sequencing Center. Briefly, sequencing libraries were generated from 100 ng of genomic DNA using the Accel-NGS 2S DNA Library Kit (Swift Biosciences, Ann Arbor, MI) on a Biomek FX robot (Beckman Coulter, Eldersburg, MD) with median insert size of 250 bp. Libraries were then dual-indexed, pooled in groups of eight, and enriched with the xGen Exome Research Panel (IDT, Rockville, MD). Sequencing was performed on the HiSeq 4000 (Illumina, San Diego, CA). After demultiplexing, reads were processed according to the GATK best practices workflow using GATK v4.0.2.0 [20 (link)]. Reads were filtered for mapping quality > 20. Average sequencing coverage was 64 ×.
Dudley J.N., Hong C.S., Hawari M.A., Shwetar J., Sapp J.C., Lack J., Shiferaw H., Johnston J.J, & Biesecker L.G. (2021). Low-level variant calling for non-matched samples using a position-based and nucleotide-specific approach. BMC Bioinformatics, 22, 181.
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6

Genomic DNA Isolation from Blood Leukocytes

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Genomic DNA from peripheral blood leukocytes was isolated using the Gentra Blood Kit (Qiagen Corp, CA).
Demir K., Kattan W.E., Zou M., Durmaz E., BinEssa H., Nalbantoğlu Ö., Al-Rijjal R.A., Meyer B., Özkan B, & Shi Y. (2015). Novel CYP27B1 Gene Mutations in Patients with Vitamin D-Dependent Rickets Type 1A. PLoS ONE, 10(7), e0131376.
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