Log In Sign up
The largest database of trusted experimental protocols

Bx71 microscope

Manufactured by Olympus
Visit Supplier
Sourced in Japan

The BX71 is a high-performance upright microscope designed for a wide range of applications. It features a sturdy frame, stable operation, and versatile illumination options to accommodate various specimen types and observation techniques.

Automatically generated - may contain errors

Lab products found in correlation

Envision dual link system, by Agilent Technologies (1 mentions) Lsm 880, by Zeiss (1 mentions) Efficient navigation zen software, by Zeiss (1 mentions) Airyscan, by Zeiss (1 mentions) Bz x710 microscope, by Keyence (1 mentions) Qproteome mammalian protein prep kit, by Qiagen (1 mentions) Ecl plus kit, by GE Healthcare (1 mentions) Anti hsp70, by Cell Signaling Technology (1 mentions) Anti sarcomeric α actinin antibody, by Merck Group (1 mentions) Anti gata4 antibody, by Santa Cruz Biotechnology (1 mentions) Anti bim, by Cell Signaling Technology (1 mentions) Hrp conjugated goat anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions) Anti cd63, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Anti cd9, by Abcam (1 mentions) Mitomycin c, by R&D Systems (1 mentions) Diff quik, by Sysmex (1 mentions) Axiovert 100 microscope, by Zeiss (1 mentions) Sp8 confocal microscope, by Leica (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)

12 protocols using bx71 microscope

1

Visualizing Prospore Membrane Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Differential interference contrast (DIC) images and fluorescence images were obtained with a BX71 microscope (Olympus, Tokyo, Japan), a Quantix 1400 camera (Photometrics, Tucson, AZ), and IPLab 3.7 software (Scanalytics, Fairfax, VA). Cells were fixed with 3.7% formaldehyde when required.
Nuclear capture counting and prospore membrane perimeter measurement were performed as previously described (11 (link)). Cells expressing both HTB2-mCherry and GFP-SPO2051-91 were sporulated for 9 h, and postmeiotic cells were identified by the pattern of Htb2-mCherry localization. The percentage of prospore membranes capturing nuclei is shown for each strain. Prospore membrane perimeters were measured by ImageJ (http://rsb.info.nih.gov/ij/).
PI4P levels at the prospore membrane were tested as previously described (12 (link)), except that sporulation was induced for 7 to 9 h at 30°C. Cells carrying both pRS426-PPRC1 -GFP-PHOSH2 and pRS424-DTR1-RFP were observed under the microscope, and GFP-PHOSH2/Dtr1-mRFP colocalization was quantified.
Time-lapse imaging was performed as previously described (38 (link)). Images were captured on a Zeiss Axiovert 100 microscope equipped with a CoolSNAP HQ camera (Photometrics) at 2-min intervals with IPLab 3.6.5a software (Scanalytics). The temperature was held at 28°C during image collection. 3D stacks were performed with IPLab 3.6.5a.
Okumura Y., Nakamura T.S., Tanaka T., Inoue I., Suda Y., Takahashi T., Nakanishi H., Nakamura S., Gao X.D, & Tachikawa H. (2015). The Dysferlin Domain-Only Protein, Spo73, Is Required for Prospore Membrane Extension in Saccharomyces cerevisiae. mSphere, 1(1), e00038-15.
+ Open protocol
+ Expand
2

Histological Examination of Tumor Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor tissues recovered from each group were fixed in 10% neutral buffered formalin and embedded in paraffin. Sections were cut 4 μm thick and then were stained either by standard H&E staining or immunohistochemical staining with mAbs specific for human CEA and Ki67, and were visualized with the Envision+ dual link system (Dako) following the manufacturer's instructions. Pictures were taken using an Olympus BH2 microscope (Olympus, Tokyo, Japan) with a camera (Coolsnap3.3; Photometrics, Tucson, AZ, USA). Tissue sections of inoculation sites were examined under an Olympus BX71 microscope (Olympus, Lake Success, NY, USA) equipped with Hoffman objective lenses ( × 40; Diagnostic Instruments, Sterling Heights, MI, USA) connected to a SPOT One Digital Camera (Diagnostic Instruments). Images were then processed using Adobe Photoshop software (Adobe, San Jose, CA, USA).
Song X., Dilly A.K., Kim S.Y., Choudry H.A, & Lee Y.J. (2014). Rapamycin-enhanced mitomycin C-induced apoptotic death is mediated through the S6K1–Bad–Bak pathway in peritoneal carcinomatosis. Cell Death & Disease, 5(6), e1281-.
+ Open protocol
+ Expand
3

Paraffin Sectioning and Histological Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Paraffin serial sections (5 μm) were prepared from cortex and myocardial tissues. Sections were de-waxed and rehydrated. Endogenous peroxidase was quenched for 20 min using 3% (v/v) hydrogen peroxide in phosphate-buffered saline (PBS). Staining was performed using H&E and hematoxylin basic fuchsin picric (HBFP). Histological images were captured with a BX71 microscope (Olympus, Tokyo, Japan).
Liu X., Tao Y., Wang F., Yao T., Fu C., Zheng H., Yan Y., Liang X., Jiang X, & Zhang Y. (2017). Kudiezi injection mitigates myocardial injury induced by acute cerebral ischemia in rats. BMC Complementary and Alternative Medicine, 17, 8.
+ Open protocol
+ Expand
4

Histological Examination of Liver Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
The liver tissue was excised, washed with ice-cold phosphate-buffered saline (PBS) and placed in 10% formalin. Several sections of tissue (thickness of 4–5 μm) were prepared and stained with hematoxylin and eosin (H and E) for histopathology and were visualized by an Olympus BX71 microscope (Hachioji, Japan).
Xu J., Wang X., Cao K., Dong Z., Feng Z, & Liu J. (2017). Combination of β-glucan and Morus alba L. Leaf Extract Promotes Metabolic Benefits in Mice Fed a High-Fat Diet. Nutrients, 9(10), 1110.
+ Open protocol
+ Expand
5

Microscopy Imaging Protocols for Osh2-P4M and PSM

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Differential interference contrast (DIC) images and fluorescence images were obtained with a BX71 microscope (Olympus, Tokyo, Japan), a Quantix 1400 camera (Photometrics), and IPLab 3.7 software (Scanalytics). In observation of Osh2-P4M, images were obtained with a BZ-X710 microscope (Keyence, Osaka, Japan), and processed with Fiji (ImageJ; National Institutes of Health, http://rsb.info.nih.gov/ij/). PSM perimeter measurements were performed as previously described [34 (link)]. PSM perimeters were measured by Fiji. Confocal images were obtained with a ZEISS LSM 880 with Airyscan (Carl Zeiss, Oberkochen, Germany) with ZEISS Efficient Navigation (ZEN) software (Carl Zeiss).
Nakamura T.S., Suda Y., Muneshige K., Fujieda Y., Okumura Y., Inoue I., Tanaka T., Takahashi T., Nakanishi H., Gao X.D., Okada Y., Neiman A.M, & Tachikawa H. (2021). Suppression of Vps13 adaptor protein mutants reveals a central role for PI4P in regulating prospore membrane extension. PLoS Genetics, 17(8), e1009727.
+ Open protocol
+ Expand
6

Histological Analysis of Lung Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
The lung tissues were fixed in 4% paraformaldehyde for 12 h, dehydrated using a graded ethanol series, and then placed in xylene for 2 h, followed by paraffin embedding overnight. Sections (4 µm) were prepared and mounted on slides, and the samples were stained with H&E for 10 min at room temperature and examined under an Olympus BX71 microscope (Olympus, Tokyo, Japan).
Mean linear intercept (MLI) and mean alveolar number (MAN) were examined as previously described (10 (link)). The MLI was used to estimate the average diameter of a single alveolus by using the formula MLI = total length/alveolar septal number. The intercepts of the alveolar septal number were counted at the intersection point of the two lines, and the total length of all of the lines combined divided by the number of intercepts provided the mean linear intercept for the region studied. The MAN was an indicator for the density of the alveoli, which was determined as the number of alveolar per square area in the field.
Zhou X., Yi D., Wu Y., Pei X., Yu H., Chen Y., Jiang Y, & Li W. (2018). Expression of diaphragmatic myostatin and correlation with apoptosis in rats with chronic obstructive pulmonary disease. Experimental and Therapeutic Medicine, 15(3), 2295-2300.
+ Open protocol
+ Expand
7

Protein extraction and western blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
Proteins were extracted from different cells or exosomes using the Qproteome Mammalian Protein Prep Kit (QIAGEN). Denatured proteins (30 μg) were separated using 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto a polyvinylidene difluoride membrane. The membrane was incubated with the following primary antibodies: polyclonal rabbit anti-PTEN, anti-BIM, anti-HSP70, anti-β-actin (Cell Signaling), anti-CD63 (Santa Cruz), and anti-CD9 (Abcam) at 4°C overnight. This was followed by incubation with HRP conjugated goat anti-rabbit secondary antibody (Cell Signaling). The membrane was washed and developed using the ECL plus kit (GE Healthcare). Densitometric analysis of the blots was performed using FluoChem SP software (Alpha Innotech).
For immunostaining, cells cultured on cover slides and sections of the myocardium were first incubated with mouse monoclonal anti-sarcomeric α-actinin antibody (Sigma) or rabbit polyclonal anti-GATA-4 antibody (Santa Cruz). Secondary antibody of goat anti-mouse IgG (FITC-conjugated) or goat anti-rabbit IgG (Alexa-568 conjugated) was then applied. Nuclei were stained with 4′,6-diamino-2-phenylindole (DAPI). Fluorescent images were obtained using an Olympus BX 71 microscope equipped with a digital camera (Olympus).
Yu B., Kim H.W., Gong M., Wang J., Millard R.W., Wang Y., Ashraf M, & Xu M. (2014). Exosomes Secreted from GATA-4 Overexpressing Mesenchymal Stem Cells Serve as a Reservoir of Anti-Apoptotic microRNAs for Cardioprotection. International journal of cardiology, 182, 349-360.
+ Open protocol
+ Expand
8

Histological Examination of Liver Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver tissues were removed, rinsed with chilled phosphate-buffered saline (PBS), then fixed in 10% paraformaldehyde solution. The treated-tissues have been embedded in paraffin, sectioned (thickness 4–5 μm), stained with hematoxylin and eosin (H&E), and visualized by Olympus BX71 microscope.
Chen C., Kang M., Wang Q., Liu W., Yang M., Liang S., Xiang Q., Han X, & Tao J. (2021). Combination of Anoectochilus roxburghii Polysaccharide and Exercise Ameliorates Diet-Induced Metabolic Disorders in Obese Mice. Frontiers in Nutrition, 8, 735501.
+ Open protocol
+ Expand
9

Wound Healing Assay with miR-29b and HSP47

Check if the same lab product or an alternative is used in the 5 most similar protocols
The A549 cells were seeded into 6-well cell culture plates. The cell culture was allowed to reach approximately 90% confluence, and then placed for 24 h in a serum-free RPMI-1640 medium for serum starvation. Next, the cells were treated with mitomycin C (5 µg/mL; R&D Systems, Minneapolis, MN, USA) for 15 min to prevent cell proliferation. A sterile 200-µL yellow pipette tip (Labcon, Petaluma, CA, USA) was used to make a straight scratch. Cell debris was washed out with the serum-free media and was further transfected with miR-29b mimic or siHSP47 before treatment with TGF-β1 for 72 h. The cells were stained with Diff-Quik (Sysmex, Kobe, Japan), and images of the migrated cells were acquired using an Olympus BX71 microscope (Olympus, Tokyo, Japan). In the micrograph, the area in between the lines refers to the area of the initial wound scratch, and cells identified between the scratch lines were counted with ImageJ analyzer (National Institutes of Health [NIH], Bethesda, MD, USA).
Shin J.M., Park J.H., Yang H.W., Moon J.W., Lee H.M, & Park I.H. (2021). miR-29b Regulates TGF-β1-Induced Epithelial–Mesenchymal Transition by Inhibiting Heat Shock Protein 47 Expression in Airway Epithelial Cells. International Journal of Molecular Sciences, 22(21), 11535.
+ Open protocol
+ Expand
10

Quantitative Analysis of Prospore Membrane Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Differential interference contrast (DIC) images and fluorescence images were obtained with a BX71 microscope (Olympus, Tokyo, Japan), a Quantix 1400 camera (Photometrics), and IPLab 3.7 software (Scanalytics).
Nuclear capture counting and prospore membrane perimeter measurement were performed as previously described (Okumura et al., 2016 ). Cells expressing both Htb2-mCherry and GFP-Spo2051–91 were sporulated for 9 h, and postmeiotic cells were identified by the pattern of Htb2-mCherry localization. The percentage of prospore membranes capturing nuclei is shown for each strain. Prospore membrane perimeters were measured by ImageJ (http://rsb.info.nih.gov/ij/).
Time-lapse imaging was performed as previously described (Ishihara et al., 2009 (link)). Images were captured on a Zeiss Axiovert 100 microscope (Carl Zeiss, Oberkochen, Germany) equipped with a CoolSNAP HQ camera (Photometrics) at 2-min intervals with IPLab 3.6.5a software (Scanalytics). The temperature was held at 28°C during image collection. Three-dimensional stacks were performed with IPLab 3.6.5a.
Nakamura T.S., Numajiri Y., Okumura Y., Hidaka J., Tanaka T., Inoue I., Suda Y., Takahashi T., Nakanishi H., Gao X.D., Neiman A.M, & Tachikawa H. (2017). Dynamic localization of a yeast development–specific PP1 complex during prospore membrane formation is dependent on multiple localization signals and complex formation. Molecular Biology of the Cell, 28(26), 3881-3895.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!