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Lumen 200 fluorescence illumination system

Manufactured by Prior Scientific
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Sourced in Japan, United Kingdom

The Lumen 200 Fluorescence Illumination System is a compact and versatile light source designed for fluorescence microscopy applications. It provides a stable and uniform illumination of the sample, enabling high-quality fluorescence imaging. The system incorporates a high-power LED light source and is equipped with a range of filter cubes to accommodate various fluorescent dyes and probes.

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Lab products found in correlation

Lsm 710 confocal microscope, by Zeiss (3 mentions) Axiocam mrc camera, by Zeiss (2 mentions) Mvx10 macroview microscope, by Zeiss (2 mentions) Eclipse ts100, by Prior Scientific (2 mentions) Polyjet in vitro dna transfection reagent, by SignaGen (1 mentions) Anti β tubulin cy3, by Merck Group (1 mentions) Anti alix, by Santa Cruz Biotechnology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Bx53 microscope, by Olympus (1 mentions)

Close spelling variants of this product

Lumen 200 (5 citations)

6 protocols using lumen 200 fluorescence illumination system

1

Multicolor Fluorescent Imaging

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Images for figure 3c were acquired using a Olympus MVX10 Macroview microscope equipped with a Zeiss AxioCam MRc camera. Flurophores were excited using a Lumen200 fluorescence illumination system (Prior Scientific) and we used the following Olympus filter sets: CFP (U-M40001XL), YFP (U-M49003XL) and mCherry (U-M49008XL).
Potvin-Trottier L., Lord N.D., Vinnicombe G, & Paulsson J. (2016). Synchronous long-term oscillations in a synthetic gene circuit. Nature, 538(7626), 514-517.
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2

Multicolor Fluorescent Imaging

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Images for figure 3c were acquired using a Olympus MVX10 Macroview microscope equipped with a Zeiss AxioCam MRc camera. Flurophores were excited using a Lumen200 fluorescence illumination system (Prior Scientific) and we used the following Olympus filter sets: CFP (U-M40001XL), YFP (U-M49003XL) and mCherry (U-M49008XL).
Potvin-Trottier L., Lord N.D., Vinnicombe G, & Paulsson J. (2016). Synchronous long-term oscillations in a synthetic gene circuit. Nature, 538(7626), 514-517.
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3

Stable Transfection of AR Variants in Prostate Cancer Cells

Cited 1 time
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LNCaP and C4–2 cells were stably transfected as described previously (20 (link)). Briefly, C4–2 or LNCaP cells at 70–80% confluency were transfected with 1 μg GFP-ARWT or GFP-ARF876L in 6-well plates using PolyJet™ In Vitro DNA Transfection Reagent (SignaGen) according to the manufacturer’s protocol. Twenty-four hours after transfection, half of the cells from each well were reseeded in 10 cm dishes containing 10 ml regular RPMI 1640 medium. After another 24 hours, medium was replaced with RPMI1640 containing 1000 μg/ml G418 (for C4–2) or 500 μg/ml (for LNCaP) for selection of stably transfected cells. The selection medium was changed every week until the colonies formed were about 1 mm in diameter. Single colonies were transferred individually to separate wells in a 96-well plate. As the colonies were expanded, each was reseeded into a 48-well, followed by a 12-well, then a 10 cm dish. The C4–2 GFP-ARWT, C4–2 GFP-ARF876L and LNCaP GFP-ARF876L stable sublines were monitored using fluorescence microscopy (Nikon Eclipse TS100, Prior Scientific Lumen 200 Fluorescence Illumination System, Tokyo, Japan) and confirmed by western blotting. Unfortunately, we were not able to generate LNCaP-GFP-ARWT, because LNCaP-GFP-ARWT cells did not propagate.
Wu Z., Wang K., Yang Z., Pascal L.E., Nelson J.B., Takubo K., Wipf P, & Wang Z. (2019). A novel androgen receptor antagonist JJ-450 inhibits enzalutamide-resistant mutant ARF876L nuclear import and function. The Prostate, 80(4), 319-328.
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4

Establishing Stable GFP-NTD Cell Lines

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C4–2 cells were transfected with 2.0 μg GFP-NTD or pEGFP-C1 in 6-well plates at 70–80% confluency. After 24 hours of transfection, half of the cells from each well were reseeded in 10 cm dishes containing 10 ml regular RPMI1640 medium. After another 24 hours, original medium was replaced by RPMI1640 containing 1000 μg/ml G418 for selection of stably transfected cells. The selection medium was changed every week until the colonies formed were large enough to be picked up. One single colony was transferred to one well of a 96-well plate. As the colony expanded, it was reseeded to a 48-well, followed by a 12-well, then a 10 cm dish. The C4–2 GFP-NTD or GFP stable cell lines were monitored under fluorescence microscopy (Nikon Eclipse TS100, Prior Scientific Lumen 200 Fluorescence Illumination System) and confirmed by Western blotting.
Dong J., Wu Z., Wang D., Pascal L.E., Nelson J.B., Wipf P, & Wang Z. (2018). Hsp70 binds to the androgen receptor N-terminal domain and modulates the receptor function in prostate cancer cells. Molecular cancer therapeutics, 18(1), 39-50.
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5

Immunofluorescence for MBR Analysis

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Cells were seeded onto poly-L-lysine–coated coverslips, fixed in 2% formaldehyde or in ice-cold methanol, permeabilized in 0.25% Triton X-100 in PBS for 10 min, and then blocked in 5% bovine serum albumin in PBS for 1 h before the primary antibody (Ab) was applied. Employed Abs were as follows: anti-ALIX (1:100; #sc-53538; Santa Cruz Biotechnology), anti-CEP55 (1:700; # 00055165-A01; Bionova); anti–β-tubulin-Cy3 (Sigma–Aldrich), secondary 488- or 594-conjugated Abs (1:300; Alexa-Flour, Life Technologies). DAPI (Sigma–Aldrich) was used to stain DNA. Cells were examined under an upright Olympus BX53 microscope equipped with a Lumen 200 Fluorescence Illumination System (Prior Scientific) with a 200-W metal arc lamp, and photographs were taken (× 100 or × 60 objectives) using a cooled camera device (ProgRes MF). Images for each sample were taken selecting the appropriate Olympus filters (DAPI: U-MNU2; FITC: U-MNB2; Texas red: U-MWIY2) at 100% of excitation light intensity with different exposure time for tubulin, CEP55, ALIX, and DAPI, respectively, of 500, 640, 1,264, and 16 ms. For MBR quantification, because CEP55 and ALIX also label centrosome, bonafide MBRs were considered only those clearly showing costaining of CEP55 or ALIX with β-tubulin that is strongly enriched in this subcompartment.
Sardina F., Monteonofrio L., Ferrara M., Magi F., Soddu S, & Rinaldo C. (2020). HIPK2 Is Required for Midbody Remnant Removal Through Autophagy-Mediated Degradation. Frontiers in Cell and Developmental Biology, 8, 572094.
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6

Multimodal Imaging of Electroporated Embryos

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Brightfield and epifluorescence imaging were carried out using an Olympus BX51 compound light microscope connected to a Lumen 200 Fluorescence Illumination System (Prior Scientific, Cambridge, UK). Brightfield images were acquired using differential interference contrast.
High-resolution imaging of transverse sections of electroporated embryos was carried out using a Zeiss LSM710 confocal microscope at 9 20 and 9 40 magnification.
Smith A.C., Fleenor S.J, & Begbie J. (2015). Changes in gene expression and cell shape characterise stages of epibranchial placode-derived neuron maturation in the chick. Journal of anatomy, 227(1).
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