Tsq quantiva triple quadrupole ms

Manufactured by Thermo Fisher Scientific

Sourced in United States

The TSQ Quantiva triple quadrupole mass spectrometer is a highly sensitive and precise analytical instrument designed for quantitative analysis. It features a triple quadrupole configuration that allows for the selective detection and quantification of target analytes in complex samples.

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Lab products found in correlation

Luna nh2 column, by Phenomenex (1 mentions) Tracefinder 2, by Thermo Fisher Scientific (1 mentions) Atlantis hilic silica column, by Waters Corporation (1 mentions) Beh amide column, by Waters Corporation (1 mentions)

Close spelling variants of this product

TSQ Quantiva (80 citations) Quantiva (9 citations) TSQ Quantiva Triple Quadrupole (3 citations) TSQ Quantiva triple (3 citations) TSQ Quantiva MS/MS (3 citations) Quantiva triple quadrupole (3 citations)

2 protocols using tsq quantiva triple quadrupole ms

Metabolite Profiling of Cell Culture Media

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10 μL of extracted cultured media were separated using a
150 × 2.0 mm Luna NH2 column (Phenomenex); mobile phase A: 20mM
ammonium acetate, 20mM ammonium hydroxide in water; mobile phase B: l0mM
ammonium hydroxide in 10% water/22.5% methanol/67.5% acetonitrile. The
column was eluted isocratically with a linear gradient from 100% to 0%
mobile phase B over 10 minutes, then 2 minutes at 0% mobile phase B. MS
data were acquired with multiple reaction monitoring in the negative ion
mode on a TSQ Quantiva triple quadrupole MS (Thermo Fisher Scientific).
Raw data were integrated and visually inspected using TraceFinder 2.1
software (Thermo Fisher Scientific). MRM transitions were as follows:
pyruvate (87/43), lactate (89.1/43.1), lactate-d3 (92.1/45.1).
Metabolite identifications were confirmed using synthetic mixtures of
reference compounds.

Kim W., Deik A., Gonzalez C., Gonzalez M.E., Fu F., Ferrari M., Churchhouse C.L., Florez J.C., Jacobs S.B., Clish C.B, & Rhee E.P. (2019). Polyunsaturated Fatty Acid Desaturation Is a Mechanism for Glycolytic NAD+ Recycling. Cell metabolism, 29(4), 856-870.e7.

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Multimodal Metabolite Profiling of Tissues

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Metabolites in the serum, liver, and muscle tissues collected from the studied rats were profiled by performing UPLC-MS analysis. Briefly, the liver and muscle samples were homogenized completely and centrifuged to generate supernatant. The resulting clear supernatant of the serum, liver, and muscle samples was transferred into the UPLC vials. Prior to examination of the experimental samples, quality controls were run to stabilize the HPLC equipment. For screening on untargeted metabolites, in positive mode, Atlantis HILIC Silica column (Waters, USA) was used; in negative mode, a BEH Amide column (Waters, USA) was used for metabolites separation. The MS methods were conducted on Q Extract Orbitrap MS screening according to the manufacturer's manual. To screen the targeted metabolites, Synergi Hydro-RP 100A column (Phenomenex, USA) and TSQ Quantiva Triple Quadrupole MS (Thermo Fisher, CA) with positive/ negative ion switching were used.

Xu Z., Zhang L., Li J., Zhao Y, & Chen X. (2018). Metabonomic Profiling Reveals Difference in Altered Metabolic Pathways Between Chronic Kidney Disease and High-Fat-Induced Insulin Resistance in Rats. Kidney & blood pressure research, 43(4).

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