Rabbit anti fak

Western blotting was performed as previously described [54 (link)]. The samples were lysed in lysis buffer (1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1 mM EGTA, 150 mM NaCl, 10% glycerol, 1% Triton X-100, 100 mM NaF, 1 mM vanadate, and protease inhibitors) to lyse cells and fresh brain tissue. The primary antibodies used were: mouse anti-DCC (1:500, #554223, BD, San Jose, CA, USA ), mouse anti-His (1:1000, TA-02, ZSGB-BIO, Beijing, China), mouse anti-myc (1:1000, 22E8, Sungene Biotech, Tianjin, China), mouse anti-Netrin-1 (1:1000, ALX804838, Enzo Life Sciences, Farmingdale, New York, USA ), mouse anti-flag (1:1000, F3165, Sigma Aldrich, St Louis, MO, USA), rabbit anti-phospho-FAK(pTyr861) (1:1000, F9176, Sigma Aldrich, St Louis, MO, USA), rabbit anti-FAK (1:200, sc-557, Santa Cruz, Santa Cruz, CA, USA), rabbit anti-phospho-SRC (Tyr418)(1:1000, Invitrogen, Carlsbad, CA, USA), rabbit anti-SRC Family (1:1000, #2123, CST, Danvers, MA, USA), mouse anti-GAPDH (1:5000, TransGene Biotech, Beijing, China), mouse anti-beta actin (1:3000, A5441, Sigma Aldrich, St Louis, MO, USA), rabbit anti-phospho-ERK(Thr202/Tyr204) (1:1000, #9101, CST, Danvers, MA, USA), rabbit anti-ERK (1:1000, #4695, CST, Danvers, MA, USA), HRP-conjugated secondary antibody against mouse, goat or rabbit were purchased from Jackson ImmunoResearch (West Grove, PA, USA).

The following antibodies were used: mouse anti-paxillin (BD Biosciences, San Jose, CA), rabbit anti-Cas, rabbit anti-FAK, rabbit anti-GAPDH and mouse anti-tubulin (Santa Cruz Biotechnology, Inc., Santa Cruz, CA), mouse anti-vinculin (Sigma-Aldrich), rabbit anti-phospho-p130 Cas (pTyr165) (Cell Signaling Technology, Beverly, MA); mouse anti-T7 (EMD Millipore), rat anti-tyr-tubulin and mouse anti-clathrin (Abcam, Cambridge, MA), mouse anti-HA (HA.11) (BioLegend, Dedham, MA) and rabbit anti-HA (Bethyl, Montgomery, TX). Mouse monoclonal to mCherry was courtesy of Ben Hoffstrom (FHCRC antibody facility) and Jihong Bai-.