We used ELISA to measure 8-isoprostanes (Cayman Chemical, Ann Arbor, MI, USA) and c-reactive proteins (CRP) (R&D Systems, Minneapolis, MN, USA) levels and biochemical assays to measure creatine kinase (CK) levels, as described previously [5 (link)].
C reactive proteins
Manufactured by R&D Systems
Sourced in United States
C-reactive proteins (CRP) are a class of proteins found in the blood, the levels of which rise in response to inflammation. They are produced by the liver and regulated by cytokines. CRP is considered a general biomarker of inflammation in the body.
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Lab products found in correlation
2 protocols using c reactive proteins
1
Biomarker Measurement Protocol
2
Plasma Biomarker Measurement Protocol
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The blood samples were collected in morning hours following an overnight fast, centrifuged at 3000 rpm for 20 min, and plasma was stored at −80 °C for further analysis, as described previously [24 ]. We used an ELISA kit to measure plasma zonulin (Cat #K5601, Immundiagnostik AG, Bensheim, Germany), according to the manufacturer's instructions [15 ]. We used ELISA kits to measure plasma c-reactive proteins (CRP) (R&D Systems, Minneapolis, MN, USA), 8–isoprostanes (Cayman Chemical, Ann Arbor, MI, USA), and creatine kinase, as described previously [17 ,18 ].
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