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Hhs128

Manufactured by Merck Group
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The HHS128 is a laboratory equipment product manufactured by Merck Group. It is designed for performing specific laboratory functions. The core function of the HHS128 is to provide precise measurements and data collection for research and analysis purposes. Further details on the intended use of this product are not available.

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Lab products found in correlation

Bz x810 microscope, by Keyence (2 mentions) Luxol fast blue (lfb), by Thermo Fisher Scientific (2 mentions) Lithium carbonate, by Thermo Fisher Scientific (2 mentions) Eclipse 80i, by Nikon (1 mentions) Dab substrate chromogen system, by Merck Group (1 mentions) Avidin biotin blocking kit, by Vector Laboratories (1 mentions) Ab485606, by Abcam (1 mentions) Ab48508, by Abcam (1 mentions)

4 protocols using hhs128

1

E-cadherin Immunohistochemistry in Mouse Embryos

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Control and null embryos were harvested at E11.5, fixed, dehydrated, embedded in paraffin, sectioned at 5μm as described for laminin staining and subject to immunohistochemistry for E-cadherin. Antigen retrieval was performed using a heat induced citrate buffer, slides were rinsed in 3% hydrogen peroxide/PBS to block endogenous peroxidase, washed in 0.5% Triton X-100/PBS, nonspecific background staining blocked using 10%BSA incubation of 60 minutes, and incubated overnight at 4°C at 1:50 concentration using monoclonal mouse anti-E-cadherin antibody (BD Pharmingen catalog #610181). Slides were then incubated in Mach 2 goat anti-mouse IgG HRP conjugated polymer (Biocare Medical catalog #MHRP520H) for 40 minutes, developed with DAB (Dako’s DAB substrate-chromogen system catalog #K3466) for two minutes, washed, and counterstained with hematoxylin (Sigma-Aldrich catalog #HHS128). Slides were imaged on a Nikon® Eclipse 80i light microscope.
Kowalkowski A., Zaremba K.M., Rogers A.P., Hoffman O.R., Turco A.E, & Nichol P.F. (2020). Lack of discreet co-localization of epithelial apoptosis to the atretic precursor in the colon of the Fibroblast growth factor receptor 2IIIb mouse and staining consistent with cellular movement suggest a revised model of atresia formation.. Developmental dynamics : an official publication of the American Association of Anatomists, 249(6), 741-753.
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2

Tissue Histopathology and Oxidative Stress

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Tissue was harvested and fixed in 10% neutral buffered formalin, dehydrated and infiltrated with paraffin and sectioned at 4 μm thickness. General structure was demonstrated by staining with the Hematoxylin-Eosin procedure (HHS-128, Sigma, St. Louis MO). Picrosirius red staining was used to identify collagen deposition (Polysciences #09400, Warrington PA). For immunohistochemistry, sections were pretreated to quench endogenous peroxidase (H2O2 in methanol) and endogenous biotin (Avidin-Biotin blocking kit, Vector Laboratories, Burlingame, CA). Oxidative damage was assessed with antibodies against 4-hydroxynonenal (ab485606, Abcam, Cambridge MA, 1:2000 dilution) and 8 Hydroxyguanosine (ab48508, Abcam, Cambridge MA, 1:100 dilution)
Call J.A., Donet J., Martin K.S., Sharma A.K., Chen X., Zhang J., Cai J., Galarreta C.A., Okutsu M., Du Z., Lira V.A., Zhang M., Mehrad B., Annex B.H., Klibanov A.L., Bowler R.P., Laubach V.E., Peirce S.M, & Yan Z. (2017). Muscle-derived extracellular superoxide dismutase inhibits endothelial activation and protects against multiple organ dysfunction syndrome in mice. Free radical biology & medicine, 113, 212-223.
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3

Spinal Cord Myelin Visualization

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Mice were euthanized with CO2 and immediately transcardially perfused with 20 mL 1X PBS followed by 20 mL 10% neutral buffered formalin (NBF; Azer Scientific). Spinal columns were dissected and immersion fixed in 10% NBF for at least 48 h. The thoracic region of the spinal cord was carefully dissected from the column, embedded in paraffin, sectioned coronally from the rostral end of each piece, and mounted on slides. Sections were stained with Luxol Fast Blue (LFB; Thermo Fisher, 212–170-250) to label myelin. In brief, after deparaffinization, sections were incubated at 60°C for 16–24 h in 0.1% Luxol Fast Blue in 95% ethanol +0.05% acetic acid. Excess stain was removed with 95% ethanol and slides were dipped in 0.05% lithium carbonate (Thermo Fisher, 446–322–500) in dH2O for 10–20 s then in 70% ethanol to remove LFB staining from gray matter but not from myelinated white matter. Slides were washed with dH2O then counterstained with hematoxylin (Sigma Aldrich, HHS128) to label nuclei. Brightfield images were acquired on a Keyence BZ-X810 microscope at 4X and 40X magnification.
Ennerfelt H., Frost E.L., Shapiro D.A., Holliday C., Zengeler K.E., Voithofer G., Bolte A.C., Lammert C.R., Kulas J.A., Ulland T.K, & Lukens J.R. (2022). SYK coordinates neuroprotective microglial responses in neurodegenerative disease. Cell, 185(22), 4135-4152.e22.
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4

Spinal Cord Myelin Visualization

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Mice were euthanized with CO2 and immediately transcardially perfused with 20 mL 1X PBS followed by 20 mL 10% neutral buffered formalin (NBF; Azer Scientific). Spinal columns were dissected and immersion fixed in 10% NBF for at least 48 h. The thoracic region of the spinal cord was carefully dissected from the column, embedded in paraffin, sectioned coronally from the rostral end of each piece, and mounted on slides. Sections were stained with Luxol Fast Blue (LFB; Thermo Fisher, 212–170-250) to label myelin. In brief, after deparaffinization, sections were incubated at 60°C for 16–24 h in 0.1% Luxol Fast Blue in 95% ethanol +0.05% acetic acid. Excess stain was removed with 95% ethanol and slides were dipped in 0.05% lithium carbonate (Thermo Fisher, 446–322–500) in dH2O for 10–20 s then in 70% ethanol to remove LFB staining from gray matter but not from myelinated white matter. Slides were washed with dH2O then counterstained with hematoxylin (Sigma Aldrich, HHS128) to label nuclei. Brightfield images were acquired on a Keyence BZ-X810 microscope at 4X and 40X magnification.
Ennerfelt H., Frost E.L., Shapiro D.A., Holliday C., Zengeler K.E., Voithofer G., Bolte A.C., Lammert C.R., Kulas J.A., Ulland T.K, & Lukens J.R. (2022). SYK coordinates neuroprotective microglial responses in neurodegenerative disease. Cell, 185(22), 4135-4152.e22.
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