The treatment group was given berberine (50 mg/kg, Med Chem Express, USA), intragastric administration once a day for 30 days. In addition to berberine, SIRT1 inhibition group was also given selisistat (20 mg/kg, Med Chem Express, USA) and was administered by intrabitoneal injection once every 3 days for 30 days. In addition to berberine, the mitochondria autophagy inhibition group was additionally given U0126-EtOH (10 mg/kg, Med Chem Express, USA) by intrabitoneal injection once every 3 days for 30 days. The mice were sacrificed to collect blood, gastrocnemius muscle, subcutaneous fat, visceral fat, and brown fat for subsequent use.
Berberine
Manufactured by MedChemExpress
Sourced in United States
Berberine is a natural compound extracted from various plant species. It functions as a fluorescent dye and can be used as a labeling reagent for biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Curcumin, by MedChemExpress (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Triptolide, by MedChemExpress (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Metformin, by MedChemExpress (1 mentions)
Erastin, by MedChemExpress (1 mentions)
Selisistat, by MedChemExpress (1 mentions)
U0126 etoh, by MedChemExpress (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle media dmem, by Merck Group (1 mentions)
Calcein acetoxymethyl ester calcein am, by Wuhan Servicebio Technology (1 mentions)
Propidium iodide, by Wuhan Servicebio Technology (1 mentions)
Super m mlv reverse transcriptase, by BioTeke (1 mentions)
Rna purified total rna extraction kit rp1201, by BioTeke (1 mentions)
Hoechst staining kit c0003, by Beyotime (1 mentions)
5 protocols using berberine
1
Berberine and Autophagy Inhibition in Mice
2
Culturing Human Gastric Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human AGS and HGC27 GC cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and maintained in F-12K (Gibco, Thermo Fisher Scientific; Waltham, USA) and RPMI 1640 (Gibco) media, respectively. Both media were supplemented with 10% fetal bovine serum (Gibco). The cells were maintained in a humidified atmosphere of 5% CO2 at 37°C. Berberine (MedChemExpress, Shanghai, China) was dissolved in dimethyl sulfoxide (Solarbio, Beijing, China) and diluted to the working concentration with culture media.
3
Leukemia Cell Culture and Treatment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human leukemia chronic myelogenous leukemia K562 and acute promyelocytic leukemia HL-60 cells were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; Hyclone; Cytiva). HEK293T (Human embryonic kidney) cells were cultured in DMEM medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Hyclone; Cytiva). All cells were purchased from The Cell Bank of Type Culture Collection of The Chinese Academy of Sciences and were grown in a medium supplemented with 100 µg/ml streptomycin (Gibco; Thermo Fisher Scientific, Inc.) and 100 U/ml penicillin (Gibco; Thermo Fisher Scientific, Inc.). Cells were maintained in a humidified 37°C incubator under a 5% CO2 atmosphere. Cells at 80% confluence were treated with 1 µg/ml DOX (cat. no. HY-15142A; MedChemExpress) for 48 h, 40 nM berberine (cat. no. HY-N0716; MedChemExpress) for 24 h, 40 nM metformin (cat. no. HY-15763; MedChemExpress), 40 nM artemisinin (cat. no. HY-B0094; MedChemExpress) for 24 h, 40 nM curcumin (cat. no. HY-N0005; MedChemExpress) for 24 h, 2 µM erastin (cat. no. HY-B0627; MedChemExpress) for 48 h and 40 nM triptolide (cat. no. HY-32735; MedChemExpress) for 24 or 48 h at 37°C.
4
Berberine and Dexamethasone for Ocular Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Berberine (BBR) was bought from MedChemExpress (Shanghai, China). Dexamethasone acetate (DEX), lipopolysaccharide (LPS), tartaric acid (TA), N-hydroxy succinimide (NHS), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) and Dulbecco’s Modified Eagle Media (DMEM) were all obtained from Sigma-Aldrich (Shanghai, China). PriMed-iCELL-001 was purchased from Ruian BioTechnologies (Shanghai, China). Fetal bovine serum (FBS) and phosphate buffered saline (PBS) were bought from Thermo Fisher (Shanghai, China). MSNs were bought from XFNANO (Nanjing, China). IRBP peptide R16 (1177–1191, ADGSSWEGVGVVPDV, 98% purity) and complete freund’s adjuvant (CFA) were synthesized by Sangon (Shanghai, China). Mycobacterium tuberculosis H37RA (TB) was bought from Difco Laboratories (Detroit, USA). The copolymer of PLGA1604–PEG1500–PLGA1604 was bought from Tanshtech Co., Ltd (Guangzhou, China). Propidium iodide (PI) and calcein acetoxymethyl ester (Calcein-AM) were bought from Servicebio (Wuhan, China). Tetracaine eye drops were purchased from Santen (Osaka, Japan). Cell Counting Kit-8 (CCK-8) assay was obtained from Dojindo Molecular (Shanghai, China). Dichloromethane and anhydrous ethanol were bought from Lingfeng Chemical Reagents Co., Ltd (Shanghai, China).
5
Berberine Biomarker Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Berberine was purchased from MedChemExpress (Monmouth Junction, NJ) and dissolved in saline. Agents for PCR detection, including RNA Purified Total RNA Extraction Kit (RP1201) and super M-MLV reverse transcriptase (RP6502) were purchased from BioTeke (Beijing, China). Agents for western blotting detection, including PMSF lysis (P0013B), protein concentration determination kit (P0012), cell cycle detection kit (C1052), CCK-8 detection kit (C0037) and Hoechst staining kit (C0003) were purchased from Beyotime Biotechnology (Shanghai, China). Cell apoptosis detection kit (KGA106) was purchased from KeyGen BioTECH (Nanjing, China). Detection kits for malondialdehyde (MDA) (A003-1), glutathione (GSH) (A006-2), glutathione peroxidase (GSH-Px) (A005) and superoxide dismutase (SOD) (A001-1) were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Detection kits for tumour necrosis factor-α (TNF-α) (EK3821/2), interleukin-1β (IL-1β) (EK301B1/2) and IL-6 (70-EK206) were purchased from Multi Sciences (Hangzhou, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!