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2000 ft ir spectrophotometer

Manufactured by PerkinElmer
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The PerkinElmer 2000 FT-IR spectrophotometer is a laboratory instrument designed to identify and analyze the chemical composition of samples through infrared spectroscopy. It measures the absorption or transmission of infrared light by a sample, providing information about the molecular structure and functional groups present.

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Lab products found in correlation

Dip 370 polarimeter, by Jasco (7 mentions) Apex 2 mass spectrometer, by Bruker (6 mentions) Spherical c18 column, by Waters Corporation (6 mentions) Uv 240 spectrophotometer, by Jasco (6 mentions) Spherical c18 100a reversed phase silica gel rp 18, by Silicycle (6 mentions) Unity plus 400, by Agilent Technologies (4 mentions) Precoated silica gel 60 f254 plates, by Merck Group (4 mentions) Silica gel 60, by Merck Group (4 mentions) Mercury 500, by Agilent Technologies (3 mentions) Vnmrs 600, by Agilent Technologies (2 mentions) Avance 3 nmr spectrometer, by Bruker (2 mentions) P 1020 polarimeter, by PerkinElmer (2 mentions) Microtof mass spectrometer, by Agilent Technologies (2 mentions) Gsx 270, by JEOL (1 mentions) Silica gel 60, by GE Healthcare (1 mentions) Lc 10ad pump, by Shimadzu (1 mentions) P 1020 digital polarimeter, by Jasco (1 mentions) Spd m10a pda detector, by Shimadzu (1 mentions) Apex 2 spectrometer, by Bruker (1 mentions) Sephadex lh 20, by GE Healthcare (1 mentions)

15 protocols using 2000 ft ir spectrophotometer

1

Synthetic Protocols for Metallorganic Complexes

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Solvents were distilled before used. The compounds 4 and 5 were carried out under N2 atmosphere. All other chemicals were purchased from commercial sources and used directly without further purification. [FcPS2]2 (Fc: Fe(η5-C5H5)(η5-C5H4) and [Cu(PPh3)2]NO3 were prepared as described in the literature
[32 ,42 ], respectively. Elemental analyses were determined with a GmbH varioMICRO CHNS apparatus. Melting points were determined by using Electrotermal apparatus. NMR spectra were recorded on a Bruker AVANCE DRX 400 NMR spectrometer and Jeol GSX 270 in CDCl3 and d6-DMSO. IR spectra was measured on a Perkin-Elmer 2000 FTIR spectrophotometer (4000 – 400 cm−1). Mass spectra were recorded with an AGILENT 1100 MSD and Waters machines. Optical rotation values were determined with an automatic digital ADP 440+ polarimeter.
Karakus M., Ikiz Y., Kaya H.I, & Simsek O. (2014). Synthesis, characterization, electrospinning and antibacterial studies on triphenylphosphine-dithiphosphonates Copper(I) and Silver(I) complexes. Chemistry Central Journal, 8, 18.
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2

Comprehensive Analytical Characterization

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Optical rotation was measured on a JASCO P-1020 digital polarimeter (Tokyo, Japan). UV data were recorded on a JASCO V-530 UV/VIS Spectrophotometer (Tokyo, Japan). High-resolution ESIMS data were obtained on a Bruker APEX II spectrometer (Billerica, MA, USA)). The IR spectrum was measured on a Perkin Elmer system 2000 FT-IR spectrophotometer (Waltham, MA, USA). The NMR spectra were obtained by JEOL JNM-ECS 400 MHz NMR (Akishima, Japan). Merck (Darmstadt, Germany) silica gel 60 and GE Healthcare (Chicago, IL, USA) Sephadex LH-20 were used for column chromatography. The instrumentation for HPLC was composed of a Shimadzu LC-10AD pump (Kyoto, Japan) and a Shimadzu SPD-M10A PDA detector.
Yu S.Y., Wang S.W., Hwang T.L., Wei B.L., Su C.J., Chang F.R, & Cheng Y.B. (2018). Components from the Leaves and Twigs of Mangrove Lumnitzera racemosa with Anti-Angiogenic and Anti-Inflammatory Effects. Marine Drugs, 16(11), 404.
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3

Characterization of Novel Organic Compounds

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Melting points were determined on the Schmelzpunkt Bestimmer Apotec melting-point apparatus using the standard open capillary method and are uncorrected. 1H, 13C and 31P NMR spectra were collected on Jeol 400yh, Bruker Avance III 500 and Bruker Avance II 600 instruments. NMR spectra recorded in CDCl3, D2O and were referenced to the respective residual 1H or 13C signals of the solvents. The reported J values are those observed from the splitting patterns in the spectrum and may not reflect the true coupling constant values. NOESY experiments were carried out at 293 K. Infrared spectra (4000–400 cm−1) were collected on a PerkinElmer 2000 FTIR spectrophotometer. High resolution mass spectra were collected using electrospray ionization on Waters LCT Premier XE TOF instrument. Optical rotations were measured using an Optical Activity Ltd. Model AA-5 automatic polarimeter; [α]D values are given in 10−1 deg cm2 g−1. Chromatographic separations were performed on silica gel 60 (70–230 mesh). Thin layer chromatography was carried out using silica gel 60 precoated plates.
Iwanejko J., Sowiński M., Wojaczyńska E., Olszewski T.K, & Górecki M. (2020). An approach to new chiral bicyclic imines and amines via Horner–Wadsworth–Emmons reaction. RSC Advances, 10(25), 14618-14629.
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4

Analytical Methods for Natural Product Research

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For the TLC, we used silica gel 60 F254-precoated plates (Merck); for column chromatography (CC), we used silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC analysis, we used a spherical C18 column (250 × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, with λmax (log ε) in nm. For optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer, with ν in cm−1. For the 1H-, 13C-, and 2D-NMR spectra, we used Varian-VNMRS-600 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
Cheng M.J., Chen J.J., Wu M.D., Leu J.Y, & Tseng M. (2023). Antifungal Activities of Compounds Produced by Newly Isolated Acrocarpospora Strains. Antibiotics, 12(1), 95.
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5

Characterization of Synthetic Compounds

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The following instruments were used for obtaining physical and spectroscopic data: optical rotations Jasco DIP-370 polarimeter; in CHCl3 (JASCO, Kyoto, Japan); FTIR spectra were obtained by using a FTIR spectrometer (Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1, Norwalk, CT, USA); Absorption spectra were recorded by an ultraviolet visible (UV-vis) light spectrophotometer (Jasco UV-240 spectrophotometer; λmax (log ε) in nm, Hitachi, Ltd., Tokyo, Japan), EI and HREIMS: Jeol JMS-HX-300 mass spectrometer; in m/z (rel. %) and JEOL SX-102A Mass Spectrometer; melting point, MP-J3 (Yanaco, Kyoto, Japan); NMR spectra were taken on a Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers with TMS as an internal standard (Lake Forest, California USA). Silica gel column chromatography was performed on silica gel (70–230 mesh, Merck, Darmstadt, Germany). HPLC was performed on a Shimadzu LDC-Analytical-III apparatus equipped with an UV–VIS detector (SPD–10A). A Spherical C18 column (250 × 10 mm, 5μm) was used for preparative purposes (flow rate: 2.00 mL/min). Aluminum pre-coated silica gel (Merck, Kieselgel 60 F254) were used for TLC monitoring with visualization by spraying with a 10% solution of Ce2SO4 in ethanol and heating to approximately 100 °C on a hotplate.
Su Y.S., Wu M.D., Chen J.J., Cheng M.J., Kuo Y.H., Chai C.Y, & Kwan A.L. (2021). Secondary Metabolites with Anti-Inflammatory Activities from One Actinobacteria Amycolatopsis taiwanensis. Molecules, 26(19), 5765.
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6

Hepatoprotective and Cytotoxic Evaluation

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The solvents; methanol and ethyl acetate of analytical reagent grade used for extraction were purchased from QRёC, Malaysia. Carbon tetrachloride; CCl4 (toxicant) and silymarin (reference hepatoprotective agent), and solvent; DMSO-d6 for the experiment of 1H and 13C NMR spectral analysis were purchased from Sigma-Aldrich Co., USA. silymarin (200 mg/kg) was dissolved in distilled water. The serum activities of alanine aminotranferase (ALT), aspartate aminotransferase (AST) and alkaline phosphate (ALP) were measured by using Hitachi 902 Automatic Chemical Analyser (Japan). The chemicals of cytotoxic activity; DBTRG (glioblastoma), MCF-7 (human breast cancer), U2OS (osteosarcoma) and PC-3 (prostate) cell lines were purchased from Sigma-Aldrich (USA). IR spectra were recorded by KBr using Perkin Elmer (USA) 2000 FT-IR spectrophotometer. The 1H and 13C NMR experiments were performed at room temperature using a Bruker Ascend 500 MHz (1H) and 125 MHz (13C) spectrometer (Bruker Biospin, Switzerland).
Jamila N., Khan N., Khan A.A., Khan I., Khan S.N., Zakaria Z.A., Khairuddean M., Osman H, & Kim K.S. (2017). IN VIVO CARBON TETRACHLORIDE-INDUCED HEPATOPROTECTIVE AND IN VITRO CYTOTOXIC ACTIVITIES OF GARCINIA HOMBRONIANA (SEASHORE MANGOSTEEN). African Journal of Traditional, Complementary, and Alternative Medicines, 14(2), 374-382.
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7

Comprehensive Analytical Techniques for Compound Characterization

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TLC: silica gel 60 F254 precoated plates (Merck, Darmstadt, Germany). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (SiliCycle, Quebec City, Canada). HPLC: Spherical C18 column (250 mm × 10 mm, 5μm) (Waters, Milford, MA, USA); LDC-Analytical-III apparatus. UV Spectra: Jasco UV-240 spectrophotometer; λmax (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl3. IR Spectra: Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. 1H-, 13C-, and 2D-NMR spectra: Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me4Si, J in Hz. ESI and HRESIMS: Bruker APEX-II mass spectrometer; in m/z.
Wu M.D., Cheng M.J., Chen J.J., Khamthong N., Lin W.W, & Kuo Y.H. (2022). Secondary Metabolites with Antimicrobial Activities from Chamaecyparis obtusa var. formosana. Molecules, 27(2), 429.
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8

Analytical Techniques for Chemical Characterization

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TLC: silica gel 60 F254 precoated plates (Merck). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). HPLC: Spherical C18 column (250 × 10 mm, 5μm) (Waters); LDC-Analytical-III apparatus. UV Spectra: Jasco UV-240 spectrophotometer; λmax (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl3. IR Spectra: Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. 1H-, 13C- and 2D-NMR spectra: Varian-Mercury-400 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. ESI and HRESIMS: Bruker APEX-II mass spectrometer; in m/z.
Su Y.S., Chen J.J., Cheng M.J., Chai C.Y., Kwan A.L., Huang J.C, & Kuo Y.H. (2021). Saccharpiscinols A–C: Flavans with Potential Anti-Inflammatory Activities from One Actinobacteria Saccharomonospora piscinae. Molecules, 26(16), 4909.
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9

Detailed Chromatographic Techniques for Compound Separation

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For TLC, we used silica gel 60 F254 precoated plates (Merck); for column chromatography (CC), silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For HPLC, we used spherical C18 column (250 × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, λmax (log ε) in nm. For the optical rotation, we used Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. For the 1H-, 13C- and 2D-NMR spectra, we used Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me4Si, J in Hz. For ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
Cheng M.J., Wu M.D., Chen J.J., Su Y.S, & Kuo Y.H. (2021). Secondary Metabolites with Antimycobacterial Activities from One Actinobacteria: Herbidospora yilanensis. Molecules, 26(20), 6236.
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10

Analytical Methods for Compound Characterization

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For the TLC, we used silica gel 60 F254-precoated plates (Merck); for column chromatography (CC), we used silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC analysis, we used a spherical C18 column (250 mm × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, with λmax (log ε) in nm. For optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer, with ν in cm−1. For the 1H-, 13C-, and 2D-NMR spectra, we used Varian-VNMRS-600 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
Wu M.D., Chen J.J, & Cheng M.J. (2023). Secondary Metabolites with Antifungal Activities from Mangrove Derived Fungus Monascus purpureus WMD2424. Marine Drugs, 21(4), 200.
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