The following conjugated mouse antibodies were used for flow cytometry analysis (clone [fluorophore]): anti-CD3 (HIT3a [FITC]), CD4 (RPA-T4 [PE and PerCP]), CD45RA (HI100 [APC]) CD25 (M-A251 [APC] or 2A3 [FITC]), CD69 (FN50 [PE]), CD127 (HIL-R7-M21 [FITC]), TNFR2 (hTNFR-M1 [PE]) and CTLA-4 (BNI3 [PE]) (BD Pharmingen, San Jose-CA, USA). The anti-GITR conjugated antibody (621 [APC]) was obtained from BioLegend. The anti-LAP conjugated antibody (27232 [PE]) was obtained from R&D Systems (Minneapolis, MN, USA) and PE conjugated anti-human FOXP3 (PCH101) was obtained from eBiosciences (San Diego, CA, USA). For FOXP3 intracellular staining, the cells were fixed and permeabilized using a Human FOXP3 Human Buffer Set kit (BD Pharmingen, San Jose-CA, USA). The reagents and cytokines used included: RPMI 1640 medium and all-trans retinoic acid from Sigma-Aldrich (St. Louis, MO, USA), recombinant human TGF-β1 and IL-2 from Peprotech (Rocky Hill, NJ, USA), anti-CD2/CD3/CD28 beads (T Cell Activation/Expansion Kit) and carboxyfluoresceinsuccinimidyl ester (CFSE) from Invitrogen (Carlsbad, CA, USA).
Pe conjugated anti human foxp3 pch101
Manufactured by Thermo Fisher Scientific
Sourced in United States
PE conjugated anti-human FOXP3 (PCH101) is a monoclonal antibody that binds to the FOXP3 transcription factor, a key regulator of regulatory T cell development and function. The antibody is conjugated to the fluorescent dye Phycoerythrin (PE), allowing for flow cytometric detection and analysis of FOXP3-expressing cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pe conjugated anti human foxp3 pch101
1
Flow Cytometry Analysis of Regulatory T Cells
2
Phenotypic Analysis of Regulatory T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Freshly isolated PBMCs of healthy volunteers and recipients at a pretransplant time point were used for phenotypic analysis of Tregs. As routine Treg analysis had only recently commenced, we analyzed Treg frequency in the patients from our cohort. Tregs were surface stained with the following fluorochrome‐conjugated monoclonal antibodies from BD Pharmingen: peridinin‐chlorophyll protein‐Cyanine5.5 anti‐human CD3 (SK715 , 16 , 17 , 18 ), allophycocyanin‐conjugated Cyanine7 anti‐human CD4 (RPA‐T4), fluorescein isothiocyanate‐conjugated anti‐human CD25 (M‐A251), PE‐Cyanine7 anti‐human CD45RA (HI100). Intracellular Foxp3 was stained using PE‐conjugated anti‐human Foxp3 (PCH101; eBioscience). Treg phenotypes were analyzed on a BD FACS Canto II (BD Biosciences), and data were analyzed with FlowJo 7.6.5 (Tree Star Inc., Ashland, OR).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!