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Pe cy7 conjugated cd3

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PE-Cy7-conjugated CD3 is a fluorescent-labeled antibody that binds to the CD3 cell surface antigen. It is designed for use in flow cytometry applications to identify and quantify T cells in samples.

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Lab products found in correlation

Percpcy5.5 conjugated f4 80, by BioLegend (1 mentions) Pe conjugated cd11b, by BD (1 mentions) Fitc conjugated cd14, by BioLegend (1 mentions) Fcr blocker, by BD (1 mentions) Facsverse system, by BD (1 mentions)

2 protocols using pe cy7 conjugated cd3

1

Multi-parameter Flow Cytometry Protocol

Cited 1 time
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Staining of surface markers was performed as described earlier [42 (link)]. The following anti-human monoclonal antibodies were used: phycoerythrin (PE)-conjugated CD33 (MACS Milteyni Biotec, Leiden, The Netherlands); PE-Cy7-conjugated CD3 (eBioscience, Vienna, Austria); allophycocyanin (APC)-conjugated CD38 (Biolegend, London, UK); APC-Fire 750-conjugated CD8α (Biolegend), CD20 (BD Biosciences, Erebodegem, Belgium); BV510-conjugated CD45 (BD Biosciences); Peridnin-chlorophyll Cy 5.5-conjugated CD4 (Biolegend); iFluor 555-conjugated MonoRab Rabbit Anti-Camelid VHH (Genscript by Bio-Connect, Huissen, The Netherlands); Vioblue-conjugated CD34 (MACS Milteyni Biotech).
De Munter S., Van Parys A., Bral L., Ingels J., Goetgeluk G., Bonte S., Pille M., Billiet L., Weening K., Verhee A., Van der Heyden J., Taghon T., Leclercq G., Kerre T., Tavernier J, & Vandekerckhove B. (2020). Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly. International Journal of Molecular Sciences, 21(3), 883.
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2

Multiparameter Flow Cytometry Profiling

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The following fluorochrome-conjugated monoclonal antibodies were used in this study: anti-mouse PE/Cy7-conjugated CD45 (Cat# 940335), APC-conjugated Ly6G (Cat# 560599), PE-conjugated CD11b (Cat# 553311), PE-conjugated CD19 (Cat# 553786), and APC/Cy7-conjugated NK1.1(Cat# 560618) (BD Biosciences, San Diego, CA, USA). PerCP/Cy5.5-conjugated F4/80 (Cat# 157317), APC/Cy7-conjugated Ly6C (Cat# 128025), FITC-conjugated CD14 (Cat# 123308), (Biolegend, San Diego, CA, USA), and PE/Cy7-conjugated CD3 (Cat# 25-0031-82) (eBioscience, San Diego, CA, USA). Cells were blocked with FcR blocker (BD Pharmingen) and then incubated with the indicated antibodies under standard protocols. All samples were analyzed using flow cytometry (FACSVerse system, BD Biosciences) with FlowJo (version 7.6.1) software.
Hou X., Liu Q., Gao Y., Yong L., Xie H., Li W., Zhou Y., Liu J., Feng L., Xu L., Shen Y, & Wang H. (2022). Mesencephalic astrocyte-derived neurotrophic factor reprograms macrophages to ameliorate acetaminophen-induced acute liver injury via p38 MAPK pathway. Cell Death & Disease, 13(2), 100.
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