DIO mice fed HFD for 12 weeks fasted for 10 h overnight then at 7 a.m., blood sample collected by RO bleed then immediately IP injected with saline or DA-GIP in escalating doses, and blood collected by RO bleed over time as indicated. Plasma metabolites corticosterone (Alpco) and glycerol (Sigma) measured over time. DA-GIP EC90 for corticosterone secretion was calculated determined following nonlinear fit of data using a sigmoidal dose-response model with variable slope (GraphPad Prism). Subsequently, DIO mice fed HFD for 12 weeks were pre-treated with vehicle or muGIPR-Ab 24-h before baseline bleed collection (T0), fasted for 10 h overnight then at 7 a.m., blood sample collected by RO bleed then immediately injected with saline or DA-GIP (80 nmol/kg), and blood collected by RO bleed as indicated. Plasma metabolites corticosterone and glycerol measured at T0 and 30 min post-dose.
Corticosterone
Manufactured by ALPCO
Sourced in United States
Corticosterone is a steroid hormone involved in the stress response. It is a metabolite of cholesterol and is produced primarily in the adrenal glands. Corticosterone plays a role in regulating various physiological processes, including metabolism, immune function, and the body's response to stress.
Automatically generated - may contain errors
4 protocols using corticosterone
1
Glucagon-Like Peptide-1 Analog Modulates Corticosterone in Diet-Induced Obese Mice
2
Hormone and Immune Marker Levels in Mice
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Testosterone, estradiol, progesterone, corticosterone, IgG, IgM, TNF-α, and IL-6 levels in mice sera were measured at the age of 14 weeks. Testosterone (R&D Systems, USA), estradiol (Cayman Chemical, USA), progesterone (Cayman Chemical, USA), corticosterone (ALPCO, USA), IgG (ICL, USA), IgM (ICL, USA), TNF-α (Anogen, Canada), IL-6 (Anogen, Canada) in sera were detected, respectively, by using the immunoassay kits according to the manufacturer's protocol.
3
Optogenetic Modulation of VMN Neurons Regulates Glycemia
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To investigate the effect of selective activation or inhibition of VMNNOS1 neurons on glycemic control, we used a 3-h approach with alternating laser off/on/off sequences in Nos1-Cre mice in a randomized, crossover manner, as previously described (11 (link)). Tail vein blood was collected for blood glucose levels at indicated times using a handheld glucometer (FreeStyle; Abbott, Santa Clara, CA). Tail blood for plasma hormonal measurement was collected at the end of the “Laser on” photoactivation (stimulation) or “Laser off” (mock) period (t = 60 min); for photoinhibition studies, blood samples for plasma hormonal measurement were collected at the end of the baseline period (t = 0) and during the study period (t = 60 min), as indicated. Tail blood was collected in EDTA-coated capillary tubes and centrifuged (10,000 rpm, 7 min), and plasma was subsequently removed and stored at −80°C for subsequent assay. Plasma insulin (Crystal Chem, Elk Grove Village, IL), corticosterone (ALPCO, Salem, NH), and glucagon (Mercodia, Winston-Salem, NC) were determined by ELISA.
4
Serum Biomarkers in Chronic Stress
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Blood samples were taken from the intra-orbital sinus after completing the 4 weeks of exposure to CUMS. Serum was obtained by centrifugation at 3 000 rpm for 15 min and was kept at –18°C.
Corticosterone (ALPCO Diagnostics, Orangeburg, NY, USA) and TNF-α, IL-6 (Quantikine R&D system, USA Kit) were assessed in the serum using enzyme linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions.
Corticosterone (ALPCO Diagnostics, Orangeburg, NY, USA) and TNF-α, IL-6 (Quantikine R&D system, USA Kit) were assessed in the serum using enzyme linked immunosorbent assay (ELISA) kits according to the manufacturer’s instructions.
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