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E11.5 pan egfp mice

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The E11.5) pan-EGFP mice are a genetically modified mouse strain that ubiquitously express the enhanced green fluorescent protein (EGFP) throughout their tissues during embryonic development. This mouse line can be used as a tool for visualizing and tracking cells during various biological studies.

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Lab products found in correlation

Collagenase dispase, by Worthington (2 mentions) Dulbecco s modified eagle medium ham s nutrient mixture f12 dmem f12, by Corning (2 mentions) Mouse egf, by Merck Group (2 mentions) N2 supplement, by Thermo Fisher Scientific (2 mentions) Basic fibroblast growth factor (bfgf), by Merck Group (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)

2 protocols using e11.5 pan egfp mice

1

Isolation and Culture of Neural Stem Cells

Cited 1 time
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NSC were harvested from 11.5 day gestational age (E11.5) pan-EGFP mice (Jackson Laboratory, Bar Harbor, ME), and cultured as described (6 (link)). Fetal intestines were dissected and digested in Collagenase/ Dispase (50 μg/ml; Worthington Biochemical, Freehold, NJ) for 1h. Centrifuged tissue pellets were retrieved and cultured in Dulbecco’s Modified Eagle Medium/Ham’s Nutrient Mixture F12 (DMEM/F12; Corning, Manassas, VA) supplemented 1:1 with N2 supplement (Gibco, Grand Island, NY), with the addition of mouse basic fibroblast growth factor (b-FGF,20 ng/ml; Sigma-Aldrich, St Louis, MO), mouse EGF (20 ng/ml; Sigma-Aldrich, St Louis, MO), 15% chicken embryo extract (Gemini, West Sacramento, CA) and 1% penicillin-streptomycin (Invitrogen, Carlsbad, CA). NSCs proliferated and grew as free-floating neurospheres, and the suspended neurospheres were collected by harvesting the culture medium. Neurospheres were confirmed to contain >95% NSC as determined by staining with the stem cell specific marker Nestin.
Wei J., Zhou Y, & Besner G.E. (2015). Heparin-Binding EGF-like Growth Factor and Enteric Neural Stem Cell Transplantation in the Prevention of Experimental Necrotizing Enterocolitis. Pediatric research, 78(1), 29-37.
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2

Isolation and Culture of Neural Stem Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
NSC were harvested from 11.5 day gestational age (E11.5) pan-EGFP mice (Jackson Laboratory, Bar Harbor, ME), and cultured as described (6 (link)). Fetal intestines were dissected and digested in Collagenase/ Dispase (50 μg/ml; Worthington Biochemical, Freehold, NJ) for 1h. Centrifuged tissue pellets were retrieved and cultured in Dulbecco’s Modified Eagle Medium/Ham’s Nutrient Mixture F12 (DMEM/F12; Corning, Manassas, VA) supplemented 1:1 with N2 supplement (Gibco, Grand Island, NY), with the addition of mouse basic fibroblast growth factor (b-FGF,20 ng/ml; Sigma-Aldrich, St Louis, MO), mouse EGF (20 ng/ml; Sigma-Aldrich, St Louis, MO), 15% chicken embryo extract (Gemini, West Sacramento, CA) and 1% penicillin-streptomycin (Invitrogen, Carlsbad, CA). NSCs proliferated and grew as free-floating neurospheres, and the suspended neurospheres were collected by harvesting the culture medium. Neurospheres were confirmed to contain >95% NSC as determined by staining with the stem cell specific marker Nestin.
Wei J., Zhou Y, & Besner G.E. (2015). Heparin-Binding EGF-like Growth Factor and Enteric Neural Stem Cell Transplantation in the Prevention of Experimental Necrotizing Enterocolitis. Pediatric research, 78(1), 29-37.
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