Deltarasin

Manufactured by Selleck Chemicals

Sourced in United States, China

Deltarasin is a laboratory chemical compound used as a research tool. It functions as a selective antagonist for the Ras-related C3 botulinum toxin substrate 1 (Rac1) protein. Deltarasin inhibits the interaction between Rac1 and its guanine nucleotide exchange factors, which are involved in regulating Rac1 activity.

Automatically generated - may contain errors

Lab products found in correlation

4 protocols using deltarasin

Molecular Pathway Inhibition Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Deltarasin (S7224), BVD-523 (S7854), AZD6244 (S1008), and BMS-754807 (S1124) were obtained from Selleck (Houston, TX, USA). ALW-II-41-27 was obtained from AbMole (Houston, TX, USA). The antibodies used in this study were as follows: antibodies against phospho-c-RAF (Ser338) (9427s), c-RAF (53745s), phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (4370L), p44/42 MAPK (ERK1/2) (4695s), phospho-AKT (Ser473) (4060s), AKT (4691s), phospho-p70 S6 kinase (Thr389) (9234s), p70 S6 kinase (2708s), phospho-EPHA2 (Tyr594) (3970s), phospho-EphA2 (Tyr588) (12677s), EPHA2 (6997s), IGF-I receptor β (3027s), phospho-IGF-I receptor β (Tyr1135) (3918s), phospho-WNK-1 (Thr60) (4946s), and phospho-GSK-3β (Ser9) (5558s) were all from Cell Signaling Technology (Cambridge, MA, USA). An antibody against KRAS (101667-T32) was purchased from Sino Biological, and an antibody against PDE6D (ab96825) was purchased from Abcam (Shanghai, China). GAPDH (60004-1-lg) was obtained from Proteintech (Rosemont, IL, USA).

Chen Y.H., Lv H., Shen N., Wang X.M., Tang S., Xiong B., Ding J., Geng M.Y, & Huang M. (2019). EPHA2 feedback activation limits the response to PDEδ inhibition in KRAS-dependent cancer cells. Acta Pharmacologica Sinica, 41(2), 270-277.

+ Open protocol

+ Expand

Molecular Mechanisms of Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Deltarasin, bafilomycin A, and 3-MA were purchased from Selleck Chemicals Co. Ltd, compound C was purchased from Sigma-Aldrich, and they were dissolved in dimethyl sulfoxide (DMSO) to a 10 or 50 mM stock concentration and stored as small aliquots at −20° C until further use. GAPDH, β-actin, C-Raf, p-C-Raf, p-AKT (Ser473), p-ERK (Thr202/Thy204), ERK, PARP, Bax, Bcl-2, p-p70s6k, p70s6k, p-AMPK, AMPK, LC3 antibodies were purchased from Cell Signaling Technology. Anti-AKT, Kras antibody was purchased from Santa Cruz Biotechnology.

Leung E.L., Luo L.X., Liu Z.Q., Wong V.K., Lu L.L., Xie Y., Zhang N., Qu Y.Q., Fan X.X., Li Y., Huang M., Xiao D.K., Huang J., Zhou Y.L., He J.X., Ding J., Yao X.J., Ward D.C, & Liu L. (2018). Inhibition of KRAS-dependent lung cancer cell growth by deltarasin: blockage of autophagy increases its cytotoxicity. Cell Death & Disease, 9(2), 216.

+ Open protocol

+ Expand

Molecular mechanisms in cell culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

D-Luciferin was from Gold Biotechnology (St. Louis, MO); lentiviral shRNA and puromycin from Santa Cruz (Dallas, TX); 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and Hoechst 33528 from Sigma-Aldrich (St. Louis, MO); mouse gene ST2.0 microarrays and relevant reagents from Affymetrix (Santa Clara, CA); recombinant cytokines and growth factors from Immunotools (Friesoythe, Germany); NF-κB-binding ELISA from Active Motif (La Hulpe, Belgium); bortezomib, IMD-0354, 17-DMAG, and deltarasin from Selleckchem (Houston, TX); G418 from Applichem (Darmstadt, Germany); IL-1β and CXCL1 ELISA from Peprotech (London, UK); and primers from VBC Biotech (Vienna, Austria). Primers, antibodies, and lentiviral shRNA pools are listed in Supplementary Tables 6–8.

Marazioti A., Lilis I., Vreka M., Apostolopoulou H., Kalogeropoulou A., Giopanou I., Giotopoulou G.A., Krontira A.C., Iliopoulou M., Kanellakis N.I., Agalioti T., Giannou A.D., Jones-Paris C., Iwakura Y., Kardamakis D., Blackwell T.S., Taraviras S., Spella M, & Stathopoulos G.T. (2018). Myeloid-derived interleukin-1β drives oncogenic KRAS-NF-κΒ addiction in malignant pleural effusion. Nature Communications, 9, 672.

+ Open protocol

+ Expand

Signaling Pathways in KRAS-Driven Cancers

Check if the same lab product or an alternative is used in the 5 most similar protocols

MDA-MB-231 (KRASG13D), SW1116 (KRASG12A), SW480 (KRASG12V), Hela (KRASwt), SW48 (KRASwt) and Colo320 (KRASwt) cells were grown in Dulbecco’s modified Eagle medium with 10% foetal calf serum (FCS; all from Gibco/Invitrogen, USA). Trametinib and Deltarasin were from Selleckchem, Simvastatin was from Sigma, Leptomycin B was from Beyotime, China. MG132 and Doxycycline were from MedChemExpress. The following antibodies were used for WB, Co-IP, IF and IHC staining: Anti-YAP, anti-MEK1/2, anti-pERK1/2, anti-Lamin B, anti-IQGAP1 and anti-Ki-67 were all from Abcam. Anti-GAPDH and anti-HA were from Santa Cruz. Anti-LATS1 and anti-β-TrCP (WB/Co-IP) were from Cell Signaling Technology and anti-β-TrCP (IF) was from Sangon Biotech, China. Anti-pMEK1, anti-CYR61 and anti-Flag were from Sangon Biotech. Anti-KRAS was from Proteintech.

Xu H., Zhou S., Xia H., Yu H., Tang Q, & Bi F. (2019). MEK nuclear localization promotes YAP stability via sequestering β-TrCP in KRAS mutant cancer cells. Cell Death and Differentiation, 26(11), 2400-2415.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!