Sampleprep 2010 geno grinder

All solvents were 99% purity or better and obtained from Thermo Fisher Scientific (Scoresby, VIC, Australia). Supelco 30/50 DVB/CAR-PDMS SPME fibers were purchased from Sigma-Aldrich (Ryde, NSW, Australia).
Unless stated otherwise, individual authentic standards and standard solutions for compound identification and were obtained from Sigma-Aldrich and Leco Australia (Baulkham Hills, NSW, Australia) as distributers for Restek (Bellefonte, CA, USA). Combined cannabis terpenes standard solutions #1 and #2 (CT, 2.5 mg/mL in isopropanol) were purchased from Restek. Dodecane (internal standard) and C₈-C₂₀ alkane mix were obtained from Sigma-Aldrich.
Cannabis plants were grown at an Agriculture Victoria Research indoor growth facility. Multiple cannabis strains from a variety of chemotypes were used for the study. Dried samples of inflorescence were ground to a fine powder using a SPEX SamplePrep 2010 Geno/Grinder for 1 min at 1500 Hz.
In a preliminary experiment, different cannabis strains were analyzed for their volatile production and compared using principal component analysis. Chemically diverse strains were selected and combined to create a test sample that represents the largest possible variety of volatile compounds.

Briefly, dried and ground cannabis inflorescences were obtained from the Victorian Government Medicinal Cannabis Cultivation Facility. Samples used were CannBio-2 (CB2), CannBio-3 (CB3), CannBio-4 (CB4), CannBio-5 (CB5) and an unnamed low THC strain. Samples were placed in liquid nitrogen for 1 min and ground to a fine powder using a SPEX SamplePrep 2010 Geno/Grinder for 1 min at 1500 rpm. This sample preparation and extraction was performed as described in a paper by Elkins et al. [19 (link)]. After grinding, 10 mg of each sample was weighed into 2 mL Axygen microtube and extracted with 1 mL of methanol, vortexed for 30 s, sonicated for 5 min and centrifuged at 13,000 rpm for 5 min. The supernatant was transferred to a 2 mL amber HPLC vial and diluted 1 in 10 to ensure responses were within the quantitative range of the instrument.