Mouse monoclonal antibodies against growth associated protein-43 (GAP43; 1:500; ab12990, Abcam) and rabbit polyclonal antibodies against MMP2 (1:200; 10373–2-AP, Proteintech) were used as primary antibodies to label growth cones and MMP2-positive structures, respectively. Cy5-conjugated anti-mouse IgG (10 µg/ml; Jackson ImmunoResearch Laboratories) and Cy3-conjugated anti-rabbit IgG (10 µg/ml; Jackson ImmunoResearch Laboratories) were used as secondary antibodies, and DyLight 488-conjugated tomato lectin (Vector Laboratories) was used with DAPI to label microglia.
Ab12990
Manufactured by Abcam
Ab12990 is a lab equipment product offered by Abcam. It is a device designed for use in scientific research and laboratory settings. The core function of this product is to provide a tool for researchers to conduct their experiments and analyses. No further details or interpretation about the intended use of this product are provided.
Automatically generated - may contain errors
Lab products found in correlation
Cy5 conjugated anti mouse igg, by Jackson ImmunoResearch (3 mentions)
Dylight 488 conjugated tomato lectin, by Vector Laboratories (2 mentions)
Cy3 conjugated anti rabbit igg, by Jackson ImmunoResearch (2 mentions)
Cy3 conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions)
Ab53554, by Abcam (1 mentions)
Alexa fluor 488 conjugated donkeyanti goat igg, by Jackson ImmunoResearch (1 mentions)
Ab6586, by Abcam (1 mentions)
3 protocols using ab12990
1
Immunolabeling of Growth Cones and MMP2
2
Immunolabeling of Growth Cones, Collagen, and Astrocytes
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Mouse monoclonal antibodies against GAP43 (1:500; ab12990, Abcam), rabbit polyclonal antibodies against collagenⅣ (1:100; ab6586, Abcam), and goat polyclonal antibodies against glial fibrillary acidic protein (GFAP; 1:100; ab53554, Abcam) were used as primary antibodies to label growth cones, collagen fibers, and astrocytes, respectively. Cy5-conjugated anti-mouse IgG (10 µg/ml; Jackson ImmunoResearch Laboratories), Cy3-conjugated donkey anti rabbit IgG (10 µg/ml; Jackson ImmunoResearch Laboratories), and Alexa Fluor 488-conjugated donkey anti goat IgG (10 µg/ml; Jackson ImmunoResearch Laboratories) were used as secondary antibodies with DAPI.
3
Labeling Growth Cones and MMP2 in Microglia
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Mouse monoclonal antibodies against GAP43 (1:500; ab12990, Abcam) and rabbit polyclonal antibodies against MMP2 (1:200; 10373–2-AP, Proteintech) were used as primary antibodies to label growth cones and MMP2-positive structures, respectively. Cy5-conjugated anti-mouse IgG (10 µg/ml; Jackson ImmunoResearch Laboratories) and Cy3-conjugated anti-rabbit IgG (10 µg/ml; Jackson ImmunoResearch Laboratories) were used as secondary antibodies, and DyLight 488-conjugated tomato lectin (Vector Laboratories) was used with DAPI to label microglia.
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