Dsictrl

HPLC-purified siRNA and DsiRNA RNAi with indicated (UU) overhangs were obtained from GE Dharmacon: (1) siCTRL sense: 5′-CGU UAA UCG CGU AUA AUA C(UU)-3′, antisense: 5′-GUA UUA UAC GCG AUU AAC G(UU)-3′; (2) DsiCTRL sense: 5′-CGU UAA UCG GCU AUA AUA CGC GUA U-3′, antisense: 5′-AUA CGC GUA UUA UAC GCG AUU AAC G(UU)-3′; (3) siSTAT3 sense: 5′-GGU CAA AUU UCC UGA GUU G(UU)-3′, antisense: 5′-CAA CUC AGG AAA UUU GAC C(UU)-3′; (4) DsiSTAT3 sense: 5′-GGU CAA AUU UCC UGA GUU GAA UUA U-3′, antisense: 5′-AUA AUU CAA CUC AGG AAA UUU GAC C(UU)-3′; (5)Chol-siCTRL: siCTRL with 3′-cholesterol conjugated to the sense strand; (6)Chol-siSTAT3: siSTAT3 with 3′-cholesterol conjugated to the sense strand; (7) Chol-DsiCTRL: DsiSTAT3 with 3′-cholesterol conjugated to the sense strand; (8) Chol-DsiSTAT3: DsiSTAT3 with 3′-cholesterol conjugated to the sense strand. For in vitro studies, lyophilized RNAi molecules were resuspended in sterile, nuclease-free ddH₂0 [100 μM] as directed (GE Dharmacon) and stored in aliquots [10 μL] at −80°C. For in vivo studies, lyophilized RNAi molecules were resuspended in the indicated buffer on the day of injection.