Ceros sperm analysis system

Manufactured by Hamilton Thorne

Sourced in United States

The CEROS sperm analysis system is a laboratory instrument designed to measure and analyze various parameters of sperm cells. It provides automated assessment of sperm concentration, motility, and other characteristics to support reproductive research and clinical applications.

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Ceros 2 sperm analysis system, by Hamilton Thorne (1 mentions)

10 protocols using ceros sperm analysis system

Sperm Velocity Analysis Protocol

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Sperm velocity was analyzed as described previously (Miyata et al., 2015 (link); Morohoshi et al., 2020 (link)). Cauda epididymal spermatozoa were dispersed in a drop of TYH medium. Sperm velocity was measured using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences) for Fam71f1 or CEROS II sperm analysis system (software version 1.4; Hamilton Thorne Biosciences) for Fam71f2 at 10 min and 2 h after incubation at 37°C under 5% CO₂. More than 200 spermatozoa were analyzed for each male.

Morohoshi A., Miyata H., Oyama Y., Oura S., Noda T, & Ikawa M. (2021). FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice. Development (Cambridge, England), 148(21), dev199644.

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Epididymal Sperm Analysis and IVF

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Protein extracts from the entire cauda epididymis were subjected to western blotting, and then the signal intensity of SLC2A3 was measured by ImageQuant TL software to estimate the sperm count within the cauda epididymis. Cauda epididymal spermatozoa were dispersed in PBS (for sperm morphology) or TYH drops {for sperm motility and in vitro fertilization (IVF)}. After an incubation period of 120 min, the sperm motility pattern was examined using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences) [39 (link)]. IVF was performed as described previously [40 (link)].

Noda T., Fujihara Y., Matsumura T., Oura S., Kobayashi S, & Ikawa M. (2018). Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity. Biology of Reproduction, 100(4), 1035-1045.

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Sperm Motility Analysis in TYH Medium

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Spermatozoa obtained from the cauda epididymis were incubated in a drop of Toyoda Yokoyama Hosi (TYH) medium (1 (link)) at 37°C under 5% CO₂. Ten minutes, 2 hours, or 4 hours after incubation, spermatozoa were collected from the top of the drop. Sperm motility was analyzed using the CEROS sperm analysis system (Hamilton Thorne Biosciences, Beverly, MA, USA). Analysis settings were used as described previously (29 (link), 49 (link)). For analyzing maximum bending angles, spermatozoa were observed with an Olympus BX-53 microscope equipped with a high-speed camera (HAS-L1, Ditect, Tokyo, Japan).

Morohoshi A., Miyata H., Tokuhiro K., Iida-Norita R., Noda T., Fujihara Y, & Ikawa M. (2023). Testis-enriched ferlin, FER1L5, is required for Ca2+-activated acrosome reaction and male fertility. Science Advances, 9(4), eade7607.

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Measuring Sperm Motility and Hyperactivation

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Cauda epididymal spermatozoa were suspended and incubated in TYH medium. Sperm motility was then measured using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences, Beverly, MA). Analysis settings were as described previously [52 (link)]. The percentage of hyperactivated spermatozoa was analyzed as described previously [53 (link)].

Sakamoto S., Thumkeo D., Ohta H., Zhang Z., Huang S., Kanchanawong P., Fuu T., Watanabe S., Shimada K., Fujihara Y., Yoshida S., Ikawa M., Watanabe N., Saitou M, & Narumiya S. (2018). mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility. PLoS Biology, 16(9), e2004874.

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Sperm Motility Analysis Protocol

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Sperm motility was analysed as described^{34 (link)}. Cauda epididymal spermatozoa were suspended in TYH medium^{35 (link),36 (link)}. Sperm motility was measured using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences, Beverly, MA, USA) at 30 min and 3 h after incubation.

Nozawa K., Satouh Y., Fujimoto T., Oji A, & Ikawa M. (2018). Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice. Scientific Reports, 8, 1315.

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Epididymal Sperm Motility Analysis

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Sperm motility analysis was performed as described previously [29 (link)]. Cauda epididymal spermatozoa were suspended and incubated in TYH medium. Sperm
motility was then measured using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences, Beverly, MA, USA). Analysis settings were as described previously [30 (link)]. The motility of epididymal spermatozoa was recorded after 5 min or 2 h of incubation in TYH medium.

SHIMADA K., KATO H., MIYATA H, & IKAWA M. (2019). Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis. The Journal of Reproduction and Development, 65(2), 155-162.

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Sperm Motility Analysis Using CEROS and High-Speed Imaging

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Sperm motility was analyzed as described previously.22 Spermatozoa obtained from cauda epididymis were incubated in the TYH medium. Sperm motility was analyzed using the CEROS sperm analysis system (Version 12.3; Hamilton Thorne Biosciences, MA, USA). Analysis settings were as described previously.23 For tracing sperm waveforms, spermatozoa were observed with an Olympus BX‐53 microscope equipped with a high‐speed camera (HAS‐L1, Ditect, Tokyo, Japan). The motility was videotaped at 200 frames per second or 50 frames per second. Obtained images were analyzed for waveforms using the sperm motion analyzing software (BohBohsoft, Tokyo, Japan).24

Miyata H., Shimada K., Morohoshi A., Oura S., Matsumura T., Xu Z., Oyama Y, & Ikawa M. (2020). Testis‐enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. The FASEB Journal, 34(4), 5389-5400.

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Sperm Velocity Measurement Protocol

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Sperm velocity was analyzed as described previously [52 (link)]. Cauda epididymal spermatozoa were suspended in TYH medium [50 ]. Sperm velocity was measured using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences, Beverly, MA, USA) at 10 min and 2 h after incubation. More than 200 spermatozoa were analyzed for each male.

Morohoshi A., Miyata H., Shimada K., Nozawa K., Matsumura T., Yanase R., Shiba K., Inaba K, & Ikawa M. (2020). Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genetics, 16(1), e1008585.

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Detailed Sperm Motility Analysis Protocol

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Sperm motility was analyzed as described previously (22 (link)). Spermatozoa obtained from cauda epididymis were incubated in TYH medium. Sperm motility was analyzed using the CEROS sperm analysis system (Version 12.3; Hamilton Thorne Biosciences, MA, USA). Analysis settings were as described previously (23 (link)). For tracing sperm waveforms, spermatozoa were observed with an Olympus BX-53 microscope equipped with a high-speed camera (HAS-L1, Ditect, Tokyo, Japan). The motility was videotaped at 200 frames per second or 50 frames per second. Obtained images were analyzed for waveforms using the sperm motion analyzing software (BohBohsoft, Tokyo, Japan) (24 ).

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Epididymal Sperm Motility Analysis

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Sperm motility analysis was performed as previously described (44 (link)). Cauda epididymal spermatozoa were suspended and incubated in Toyoda, Yokoyama and Hoshi (TYH) medium that can induce sperm capacitation (45 ). Sperm motility was then measured using the CEROS sperm analysis system (software version 12.3; Hamilton Thorne Biosciences). Analysis settings were as previously described (46 (link)). The motility of epididymal spermatozoa was recorded after 30 min and 2 h of incubation in TYH medium. Movies of control and Armc12 KO spermatozoa were recorded after 30 min incubation in TYH as previously described (44 (link)).

Shimada K., Park S., Miyata H., Yu Z., Morohoshi A., Oura S., Matzuk M.M, & Ikawa M. (2021). ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proceedings of the National Academy of Sciences of the United States of America, 118(6), e2018355118.

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