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Anti ph3

Manufactured by Santa Cruz Biotechnology
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Sourced in United States

Anti‐pH3 is a lab equipment product that detects and measures phosphorylated histone H3, a marker of cell division and mitosis. It is used for research purposes.

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Lab products found in correlation

α tubulin, by Santa Cruz Biotechnology (1 mentions) Anti ki67, by Santa Cruz Biotechnology (1 mentions) Anti cleaved caspase 3, by Abcam (1 mentions) β catenin, by Santa Cruz Biotechnology (1 mentions) Anti foxm1, by Santa Cruz Biotechnology (1 mentions) Anti v5, by Thermo Fisher Scientific (1 mentions) K02288, by Selleck Chemicals (1 mentions) Anti ki67, by Abcam (1 mentions) Anti cleaved caspase 3, by Thermo Fisher Scientific (1 mentions) Anti gapdh, by Abcam (1 mentions) Anti β tubulin, by Abcam (1 mentions) Anti egfr, by Abcam (1 mentions) Anti f actin, by Thermo Fisher Scientific (1 mentions) Repsox, by Merck Group (1 mentions) Anti smad2 3, by Santa Cruz Biotechnology (1 mentions) Anti p smad2 3, by Santa Cruz Biotechnology (1 mentions) Anti pegfr y1068, by Abcam (1 mentions) Galunisertib ly2157299, by Selleck Chemicals (1 mentions) A83 01, by MedChemExpress (1 mentions) Anti hepsin, by R&D Systems (1 mentions)

Spelling variants of this product

Rabbit anti-pH3 antibody (3 citations) Anti-pH3 primary antibody (2 citations)

3 protocols using anti ph3

1

Immunohistochemical Analysis of Cell Proliferation and Apoptosis

Cited 1 time
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Lung tissue sections were stained using anti-FOXM-1, anti-Ki-67, anti-PH3 (Santa Cruz) and anti-Cleaved Caspase-3 (Abcam) antibodies, as described previously (33 (link)). Tumor cells growing on coverslips were treated with 20 μM of RCM-1 for 24 h, fixed and stained with antibodies against FOXM1, FOXA1, β-catenin, Ki-67 and α-tubulin (Santa Cruz) as previously described (32 (link)).
Shukla S., Milewski D., Pradhan A., Rama N., Rice K., Le T., Flick M.J., Vaz S., Zhao X., Setchell K.D., Logarinho E., Kalinichenko V.V, & Kalin T.V. (2019). The FOXM1 inhibitor RCM-1 decreases carcinogenesis and nuclear β-catenin. Molecular cancer therapeutics, 18(7), 1217-1229.
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2

Inhibitors and Antibodies for Cellular Signaling

Cited 1 time
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The inhibitors used in this study were K02288 (Selleckchem, S7359, Houston, TX, USA), Galunisertib/LY2157299 (Selleckchem, S2230), RepSox (Sigma‐Aldrich/Merck, R0158), A‐83‐01 (MedChem Express, HY‐10432, Monmouth Junction, NJ, USA), Erlotinib (EGFRi, Selleckchem, S1023), and human ALK1 inhibitory antibody (R&D Systems, MAB3701). The hepsin inhibitory antibody (Ab25) was a kind gift from Dr. Kirchhofer, Genentech [10 (link)]. ZFH7116 provided by Dr. Janetka [12 (link)]. The antibodies used in this study were Anti‐sheep HRP (Upstate cell signaling solutions, #12‐342), anti‐rabbit HRP (Millipore, AP132P, Burlington, MA, USA), anti‐hepsin (R&D Systems, AF4776), anti‐GAPDH (CST#2118S), anti‐β‐tubulin (Abcam, ab6046, Cambridge, UK), anti‐pEGFR Y1068 (Abcam, ab40815), anti‐EGFR (Abcam, ab32077), anti‐pSmad2/3 (CST#8828), anti‐Smad2/3 (CST#8685), anti‐Smad2 (CST#5339), anti‐pH3 (Santa Cruz, sc‐8656, Dallas, TX, USA), total‐H3 (CST#9715), anti‐V5 (Invitrogen, #R96025), anti‐Ki67 (Abcam, ab15580), anti‐cleaved caspase 3 (CST#9661), anti‐F‐actin (phalloidin staining) (Thermo Fisher Scientific, A22283), anti‐pSmad1/5 (CST#9516) and anti‐ALK‐1 (R&D Systems, AF370).
Belitškin D., Munne P., Pant S.M., Anttila J.M., Suleymanova I., Belitškina K., Kirchhofer D., Janetka J., Käsper T., Jalil S., Pouwels J., Tervonen T.A, & Klefström J. (2023). Hepsin promotes breast tumor growth signaling via the TGFβ‐EGFR axis. Molecular Oncology, 18(3), 547-561.
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3

Double Immunofluorescence and TUNEL Assay

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Double immunofluorescence was undertaken using anti‐pH3 (Santa Cruz Biotechnology) and anti‐GPF Ab (Santa Cruz Biotechnology). Dhx15+/−; Tg(cmyb:gfp) inbred embryos were fixed overnight in 4% paraformaldehyde and washed with PBST. Embryos were penetrated by 50 μg/ml proteinase K for 15 minutes. After refixation with 4% paraformaldehyde, embryos were washed with PBST, blocked for at least 1 hour with 2% BSA, 10% lamb serum, and 1% Triton‐X100 in PBS, and incubated with anti‐pH3 (1:200) and anti‐GFP (1:200) at 4℃ overnight. After being washed with PBST, embryos were incubated with a secondary Ab (1:1000) at 4℃ overnight. Embryos were processed for imaging after being washed with PBST.
The TUNEL assay was undertaken with an In Situ Cell Death Detection Kit, TMR red (Roche). Green fluorescent protein immunostaining was carried out as described above.
Cai Y., Wang J., Jin D., Liu Q., Chen X., Pan L., Li Y., Wang X., Qian F., Wang J., Zhong T.P, & Wang S. (2021). Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway. Cancer Science, 112(9), 3884-3894.
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