All animal work was approved by Duke University’s Institutional Animal Care and Use Committee. All mice were maintained in a barrier facility with 12-hour light/dark cycles. Mice were genotyped by PCR and both males and females were analyzed. TRE-Arhgef11CA were generated in the lab as previously described [7 (link)]. Other animal strains used in this study were Villin-rtTA (Jackson Laboratories,[40 (link)]), VillinCre (Jackson Laboratories [41 (link)]), and Rosa-rtTA3 (Jackson Laboratories).
Rosa rtta3
Manufactured by Jackson ImmunoResearch
The Rosa-rtTA3 is a laboratory tool that serves as a Tetracycline-inducible Cre recombinase. It can be used to conditionally express target genes in a Tet-On system.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using rosa rtta3
1
Genetically Modified Mouse Models for Research
2
Arhgef11 Overexpression Rescue Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All mice were maintained in a barrier facility with 12-hour light/dark cycles. Mice were genotyped by PCR and both males and females were analyzed. TRE-Arhgef11CA were generated in the lab as previously described [24 (link)]. Other animal strains used in this study were Villin-rtTA (Jackson Laboratories, [42 (link)]), VillinCre (Jackson Laboratories [43 (link)]), and Rosa-rtTA3 (Jackson Laboratories). For drug rescue experiments, mice were injected with Y-27632 dihydrochloride (ApexBio A3008-50) (10 mg/kg), at 8 hours after doxycycline chow.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!