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Ready set go human ifnγ or tnfα kits

Manufactured by Thermo Fisher Scientific
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The READY-SET-GO human IFNγ or TNFα kits are laboratory reagents designed for the quantitative determination of human interferon-gamma (IFNγ) or tumor necrosis factor-alpha (TNFα) in biological samples. The kits provide the necessary components, including pre-coated plates, detection antibodies, and standards, to perform a sandwich enzyme-linked immunosorbent assay (ELISA).

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Lab products found in correlation

Human granzyme b platinum elisa kit, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Ready-SET-Go (98 citations) Ready-Set-Go kits (76 citations) IFNγ Ready-Set-Go Kit (3 citations) TNFα Kits (2 citations)

2 protocols using ready set go human ifnγ or tnfα kits

1

Quantifying Immune Cell Secretions

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Supernatants of 2D and 3D ADCC assays were harvested and frozen at −20°C. Secreted human IFNγ, TNFα, and granzyme B were measured by ELISA using the READY-SET-GO human IFNγ or TNFα kits or the Human Granzyme B platinum ELISA kit as described by the manufacturer (eBioscience).
Del Bano J., Florès-Florès R., Josselin E., Goubard A., Ganier L., Castellano R., Chames P., Baty D, & Kerfelec B. (2019). A Bispecific Antibody-Based Approach for Targeting Mesothelin in Triple Negative Breast Cancer. Frontiers in Immunology, 10, 1593.
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2

ADCC Assay Cytokine and Degranulation Analysis

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Supernatants were harvested from ADCC assays and frozen at ×20°C. Secreted human IFNγ and TNFα were measured by ELISA using the READY-SET-GO human IFNγ or TNFα kits as described by the manufacturer (eBioscience). Secreted human granzyme B was measured by ELISA using the Human Granzyme B Platinum ELISA kit (eBioscience). Cell surface expression of CD69 on harvested NK cells was analyzed by flow cytometry after labeling with an APC-conjugated anti-CD69 IgG (Miltenyi).
Turini M., Chames P., Bruhns P., Baty D, & Kerfelec B. (2014). A FcγRIII-engaging bispecific antibody expands the range of HER2-expressing breast tumors eligible to antibody therapy. Oncotarget, 5(14), 5304-5319.
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