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Sybr premier ex taq 2

Manufactured by Takara Bio
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Sourced in Japan

SYBR Premier Ex Taq II is a hot-start DNA polymerase enzyme used for real-time PCR applications. It is designed for sensitive and specific amplification of target DNA sequences.

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Lab products found in correlation

Gdna eraser, by Takara Bio (1 mentions) Rna extraction kit, by Takara Bio (1 mentions) One step primescript mirna cdna synthesis kit, by Takara Bio (1 mentions) Primescript rt reagent kit, by Takara Bio (1 mentions) Primescript rt, by Takara Bio (1 mentions) Lc480 light cycler, by Roche (1 mentions) Trizol, by Takara Bio (1 mentions)

Close spelling variants of this product

Ex Taq (400 citations) SYBR II (3 citations)

2 protocols using sybr premier ex taq 2

1

Quantitative Analysis of miRNA and mRNA Expressions

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Total RNA of cells was extracted using the RNA extraction kit (Takara, Dalian,
China) and the concentration of extracted RNA was detected. Next, miR-101-3p and
U6 were reversely transcribed into cDNA using the One Step PrimeScript miRNA
cDNA Synthesis kit (Takara). HOTTIP, STC1 and glyceraldehyde-3-phosphate
dehydrogenase (GAPDH) were reversely transcribed into cDNA using the PrimeScript
RT reagent kit with gDNA Eraser (Takara). The expressions of HOTTIP, STC1,
miR-101-3p were detected on the instructions of SYBR Premier Ex Taq II (Takara)
using qRT-PCR, with cDNA acting as the template. The relative expression of miR
and mRNAs was calculated by 2-ΔΔCt method, with U6 and GAPDH acting
as the internal reference. Primer sequences are illustrated in Table 1.
Yuan X., Sun Z, & Cui C. (2021). Knockdown of lncRNA HOTTIP Inhibits Retinoblastoma Progression by Modulating the miR-101-3p/STC1 Axis. Technology in Cancer Research & Treatment, 20, 1533033821997831.
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2

Quantitative Analysis of Cx43 Expression

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Lung tissue and RLE-6TN cells were cut in Trizol (9108, Takara, Japan) to isolate and purify the nucleic acid. The A260/A280 ratio was used to adjust the RNA concentration. Primescript RT (Takara, Japan) and SYBR Premier Ex Taq II (Takara, Japan) kits were used for reverse transcription and amplification, respectively. Detection was performed using a Roche LC480 Light Cycler with the following primers: Cx43: forward: 5′-TCT​CGC​CTA​TGT​CTC​CTC​C-3′, reverse: 5′-TGGTC CACGATGGCTAAT-3′; β-actin: forward: 5′-CGT​GCG​TGA​CAT​TAA​AGA​G-3′, reverse: 5′-TTG​CCG​ATA​GTG​ATG​ACC​T-3′. The relative amount of transcripts was calculated using the 2 (−ΔΔCt) method and normalized to β-actin transcript as an internal control.
Qing C., Xinyi Z., Xuefei Y., Xindong X, & Jianhua F. (2021). The Specific Connexin 43–Inhibiting Peptide Gap26 Improved Alveolar Development of Neonatal Rats With Hyperoxia Exposure. Frontiers in Pharmacology, 12, 587267.
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