Calciumgreen am

Manufactured by Thermo Fisher Scientific

Sourced in United States

CalciumGreen/AM is a fluorescent dye used for the detection and measurement of intracellular calcium levels in live cells. It is a membrane-permeable compound that binds to calcium ions, resulting in a fluorescent signal that can be measured using a fluorescence microscope or plate reader.

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Lab products found in correlation

Histamine, by Merck Group (2 mentions) Sfllrn, by Merck Group (2 mentions) Fura 2 am, by Thermo Fisher Scientific (2 mentions) U73343, by Bio-Techne (1 mentions) U73122, by Bio-Techne (1 mentions) Er tracker red, by Thermo Fisher Scientific (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Hrp conjugated goat anti rabbit igg antibody, by Bio-Rad (1 mentions) Wga alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Trans ned19, by Bio-Techne (1 mentions) Mitotracker deep red, by Thermo Fisher Scientific (1 mentions) Dantrolene, by Merck Group (1 mentions) U73343, by Merck Group (1 mentions) U73122, by Merck Group (1 mentions) Rs127445, by Bio-Techne (1 mentions) Bw723c86, by Bio-Techne (1 mentions) Cgs12066b, by Bio-Techne (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions) Dihydroethidium, by Thermo Fisher Scientific (1 mentions) Vas2870, by Merck Group (1 mentions)

3 protocols using calciumgreen am

Intracellular Calcium Signaling Assay

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cis-NED 19, trans-NED 19, U73122, and U73343 were acquired from Tocris (Bristol, UK); Fura2/AM, CalciumGreen/AM, and LysoTracker Red DND-99 from ThermoFischer Scientific (Waltham, MA, USA); Mitotracker Deep Red (M22426, Invitrogen), ER-tracker Red (E34250, Invitrogen), WGA Alexa Fluor 594 (Thermo Fisher, W11262), and antibodies anti-TPC1 (#ACC-071) and anti-TPC2 (#ACC-072) from Alomone Labs (Jerusalem, Israel); HRP-conjugated goat anti-rabbit IgG antibodies from BioRad (#170-6515); and the agonists of the receptors from SigmaAldrich (St. Louis, MO, USA).

Trufanov S.K., Rybakova E.Y., Avdonin P.P., Tsitrina A.A., Zharkikh I.L., Goncharov N.V., Jenkins R.O, & Avdonin P.V. (2019). The Role of Two-Pore Channels in Norepinephrine-Induced [Ca2+]i Rise in Rat Aortic Smooth Muscle Cells and Aorta Contraction. Cells, 8(10), 1144.

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Fluorescence Imaging of Cell Signaling

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CGS12066B, BW723C86, RS127445, and SB216649 were from Tocris Bioscience (Bristol, UK); 5-HT, histamine, ATP, SFLLRN, U73122, U73343, and dantrolene were from Sigma-Aldrich (St. Louis, MO, USA); CalciumGreen/AM was from Thermo Fischer Scientific (Waltham, MA, USA). The stock solutions of CGS12066B, BW723C86, RS127445, SB216649, dantrolene, U73122, and U73343 were prepared in Dimethylsulfoxide (DMSO). Before being added to the cells, they were dissolved to the required concentrations in a physiological salt solution (PSS). DMSO at appropriate concentrations was used as a vehicle control. PSS was added as a vehicle control for 5-HT, ATP, histamine, and SFLLRN.

Rybakova E.Y., Avdonin P.P., Trufanov S.K., Goncharov N.V, & Avdonin P.V. (2023). Synergistic Interaction of 5-HT1B and 5-HT2B Receptors in Cytoplasmic Ca2+ Regulation in Human Umbilical Vein Endothelial Cells: Possible Involvement in Pathologies. International Journal of Molecular Sciences, 24(18), 13833.

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Calcium Signaling Measurement Assay

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VAS2870, histamine, and SFLLRN were from SigmaAldrich (St. Louis, MO, USA); CalciumGreen/AM and dihydroethidium were from Thermo Fischer Scientific (Waltham, MA, USA). VAS2870 was dissolved at a 10 mM concentration in DMSO. Before being added to the cells, it was dissolved to the required concentrations in physiological salt solution (PSS). DMSO at appropriate concentrations was used as a vehicle control. PSS was added as a vehicle control for histamine and SFLLRN.

Avdonin P.V., Rybakova E.Y., Avdonin P.P., Trufanov S.K., Mironova G.Y., Tsitrina A.A, & Goncharov N.V. (2019). VAS2870 Inhibits Histamine-Induced Calcium Signaling and vWF Secretion in Human Umbilical Vein Endothelial Cells. Cells, 8(2), 196.

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