Wild-type (WT) OTUD7B and its inactive mutant plasmids were obtained from Hanbio Biotechnology Co., Ltd. (Shanghai, China). The HA-K6, -K11, -K27, -K29, -K33, -K48, -K63, and -Ub plasmids were acquired from Addgene. Small-interfering RNAs targeting OTUD7B (siRNA-1: 5′-CCGAUUGGCCAGUGUAAUU-3′; 5′-CCGAGUGGCUGAUUCCUAU-3′) were synthesized by Genepharma (Shanghai, China). The ERα full- and its deletion constructs were gifted from Dr. Ting Zhuang and were described in our previous study30 (link). Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) was used for plasmid transfection according to the manufacturer’s instructions.
Small interfering rnas targeting
Manufactured by GenePharma
Sourced in China
Small-interfering RNAs (siRNAs) are double-stranded RNA molecules that can be used to silence the expression of specific genes. They work by binding to and degrading the target mRNA, preventing it from being translated into a functional protein. This product from GenePharma provides synthetic siRNAs that can be used for targeted gene knockdown experiments in a laboratory setting.
Automatically generated - may contain errors
2 protocols using small interfering rnas targeting
1
OTUD7B Regulation of Estrogen Receptor
2
Plasmids and siRNAs for Ubiquitin Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The The full-length and deletion mutant constructs of TAZ and USP26 were obtained from Hanbio Biotechnology Co. Ltd. (Shanghai, China). The HA-K6, HA-K11, HA-K27, HA-K29, HA-K33, HA-K48, HA-K63, and HA-Ub plasmids were acquired from Addgene. Small interfering RNAs targeting USP26 (siRNA-1: 5′-GCACAAGACUUCCGUUGGA-3′; 5′-AAACAGAUCUGGUUCACUU-3′) were synthesized by Genepharma (Shanghai, China).
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